Research of Endothelial Progenitor Cells Labeled with Superpara-Magnetic Iron Oxide in Treatment for Venous Thrombosis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 KANGTao ¹ ,  LUYao-liang ¹ , LIXiao-qiang ² , MENGQing-you ²

1. Department of Vascular Surgery, Affiliated Taicang Hospital of Soochow University, Taicang 215400, Jiangsu Province, China;
2. Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China;

Corresponding author：

LUYao-liang, Email: bright612@163.com

Keywords：

Venous thrombosis; Endothelial progenitor cell; Superpara-magnetic iron oxide; Magnetic resonance imaging; Stem cell tracer

DOI：

10.7507/1007-9424.20140143

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To research the magnetic labeled endothelial progenitor cells(EPCs) transplanted into the rat of venous thrombosis model through the tail vein and track transplanted stem cells in vivo, provide an effective monitoring technology for promoting organization and recanalization of deep venous thrombosis. Methods Bone marrowderived EPCs were extracted, purified, and identified, then labeled with the new SPIO particles. At the same time, the inferior vena cava thrombus models in rats were made, which were randomly divided into four groups:SPIO group (EPCs labeled with SPIO transplantation), Dil group (EPCs labeled with Dil transplantation), control group (simple EPCs transplantation), and blank control group (1 mL medium transplantation). After transplantation, the MRI, HE staining, and immunohistochemical staining were performed and the capillary density was counted under high-power microscope. Results The MRI showed that EPCs labeled with SPIO had migrated to the inferior vena cava thrombus and the mass of high signal shade was seen, with the extension of time, the signal strengthened gradually. On day 14-21, the signal became the strongest, then decreased gradually. The immunohistochemical staining and HE staining showed that there were a mass of the new capillary in the specimens of thrombus of the SPIO group, Dil group, and control group, the difference was not statistically significant among these three groups(P > 0.05), but which was significant difference as compared with blank control group (P < 0.05). Conclusion Compared with EPCs labeled with Dil, it

Citation： KANGTao, LUYao-liang, LIXiao-qiang, MENGQing-you. Research of Endothelial Progenitor Cells Labeled with Superpara-Magnetic Iron Oxide in Treatment for Venous Thrombosis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(5): 593-598. doi: 10.7507/1007-9424.20140143 Copy

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13.	Wei H, Ooi TH, Tan G, et al.Cell delivery and tracking in postmyocardial infarction cardiac stem cell therapy:an introduction for clinical researchers[J].Heart Fail Rev, 2010, 15(1):1-14.
14.	Bao X, Pal R, Hascup KN, et al.Transgenic expression of Glud1(glutamate dehydrogenase 1) in neurons:in vivo model of enhanced glutamate release, altered synaptic plasticity, and selective neuronal vulnerability[J].J Neurosci, 2009, 29(44):13929-13944.
15.	Peichev M, Naiyer AJ, Pereira D, et al.Expression of VEGFR-2 and AC133 by circulating human CD34(+) cells identifies a population of functional endothelial precursors[J].Blood, 2000, 95(3):952-958.
16.	施森, 何延政, 刘勇, 等.外周血来源内皮祖细胞三维血管新生模型的建立及其特性分析[J].中国普外基础与临床杂志, 2009, 16(2):119-123.
17.	Terrovitis J, Stuber M, Youssef A, et al.Magnetic resonance imaging overestimates ferumoxide-labeled stem cell survival after transplantation in the heart[J].Circulation, 2008, 117(12):1555-1562.
18.	杨汉丰, 宋彬, 周翔平, 等.菲立磁增强MRI对局灶性肝病的定性诊断价值[J].中国普外基础与临床杂志, 2004, 11(5):454-457, 465.
19.	陈小伍, 封静, 戎祯祥, 等.菲立磁标记大鼠骨髓基质干细胞对其生物活性的影响[J].中华实验外科杂志, 2008, 25(7):866-868.
20.	Bos C, Delmas Y, Desmoulière A, et al.In vivo MR imaging of intravascularly injected magnetically labeled mesenchymal stem cells in rat kidney and liver[J].Radiology, 2004, 233(3):781-789.
21.	Anderson SA, Glod J, Arbab AS, et al.Noninvasive MR imaging of magnetically labeled stem cells to directly identify neovasculature in a glioma model[J].Blood, 2005, 105(1):420-425.
22.	司逸, 符伟国, 竺挺, 等.内皮祖细胞促动脉内皮损伤修复的研究[J].中国分子心脏病学杂志, 2010, 10(1):17-22.

1. Moldovan NI.Tissular insemination of progenitor endothelial cells:the problem, and a suggested solution[J].Adv Exp Med Biol, 2003, 522:99-113.
2. Modarai B, Burnand KG, Sawyer B, et al.Endothelial progenitor cells are recruited into resolving venous thrombi[J].Circulation, 2005, 111(20):2645-2653.
3. 李晓强, 孟庆友, 于小滨.VEGF165基因转染骨髓源性血管内皮祖细胞的体外实验研究[J].中华普通外科杂志, 2007, 122(12):936-938.
4. Li XQ, Meng QY, Wu HR.Effects of bone marrow-derived endothelial progenitor cell transplantation on vein microenvironment in a rat model of chronic thrombosis[J].Chin Med J (Engl), 2007, 120(24):2245-2249.
5. 康涛, 李晓强, 孟庆友, 等.新型超顺磁性氧化铁标记血管内皮祖细胞的实验研究[J/CD].中国血管外科杂志:电子版, 2012, 4(2):108-111, 114.
6. Bruce IJ, Sen T.Surface modification of magnetic nanoparticles with alkoxysilanes and their application in magnetic bioseparations[J].Langmuir, 2005, 21(15):7029-7035.
7. Asahara T, Murohara T, Sullivan A, et al.Isolation of putative progenitor endothelial cells for angiogenesis[J].Science, 1997, 275(532):964-967.
8. Matsumura G, Miyagawa-Tomita S, Shin' oka T, et al.First evidence that bone marrow cells contribute to the construction of tissue-engineered vascular autografts in vivo[J].Circulation, 2003, 108(14):1729-1734.
9. Isner JM, Kalka C, Kawamoto A, et al.Bone marrow as a source of endothelial cells for natural and iatrogenic vascular repair[J].Ann N Y Acad Sci, 2001, 953:75-84.
10. Weissleder R, Stark DD, Engelstad BL, et al.Superparamagnetic iron oxide:pharmacokinetics and toxicity[J].AJR Am J Roentgenol, 1989, 152(1):167-173.
11. Yeh TC, Zhang W, Ildstad ST, et al.In vivo dynamic MRI tracking of rat T-cells labeled with superparamagnetic iron-oxide particles[J].Magn Reson Med, 1995, 33(2):200-208.
12. Schäfer R, Kehlbach R, Wiskirchen J, et al.Transferrin receptor upregulation:in vitro labeling of rat mesenchymal stem cells with superparamagnetic iron oxide[J].Radiology, 2007, 244(2):514-523.
13. Wei H, Ooi TH, Tan G, et al.Cell delivery and tracking in postmyocardial infarction cardiac stem cell therapy:an introduction for clinical researchers[J].Heart Fail Rev, 2010, 15(1):1-14.
14. Bao X, Pal R, Hascup KN, et al.Transgenic expression of Glud1(glutamate dehydrogenase 1) in neurons:in vivo model of enhanced glutamate release, altered synaptic plasticity, and selective neuronal vulnerability[J].J Neurosci, 2009, 29(44):13929-13944.
15. Peichev M, Naiyer AJ, Pereira D, et al.Expression of VEGFR-2 and AC133 by circulating human CD34(+) cells identifies a population of functional endothelial precursors[J].Blood, 2000, 95(3):952-958.
16. 施森, 何延政, 刘勇, 等.外周血来源内皮祖细胞三维血管新生模型的建立及其特性分析[J].中国普外基础与临床杂志, 2009, 16(2):119-123.
17. Terrovitis J, Stuber M, Youssef A, et al.Magnetic resonance imaging overestimates ferumoxide-labeled stem cell survival after transplantation in the heart[J].Circulation, 2008, 117(12):1555-1562.
18. 杨汉丰, 宋彬, 周翔平, 等.菲立磁增强MRI对局灶性肝病的定性诊断价值[J].中国普外基础与临床杂志, 2004, 11(5):454-457, 465.
19. 陈小伍, 封静, 戎祯祥, 等.菲立磁标记大鼠骨髓基质干细胞对其生物活性的影响[J].中华实验外科杂志, 2008, 25(7):866-868.
20. Bos C, Delmas Y, Desmoulière A, et al.In vivo MR imaging of intravascularly injected magnetically labeled mesenchymal stem cells in rat kidney and liver[J].Radiology, 2004, 233(3):781-789.
21. Anderson SA, Glod J, Arbab AS, et al.Noninvasive MR imaging of magnetically labeled stem cells to directly identify neovasculature in a glioma model[J].Blood, 2005, 105(1):420-425.
22. 司逸, 符伟国, 竺挺, 等.内皮祖细胞促动脉内皮损伤修复的研究[J].中国分子心脏病学杂志, 2010, 10(1):17-22.

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Research of Endothelial Progenitor Cells Labeled with Superpara-Magnetic Iron Oxide in Treatment for Venous Thrombosis

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