miR-338-5p Is Downregulated in Colorectal Cancer and Its Role in Inhibiting Colon Cancer Cell Proliferation_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 XIONGGuang-bing , ZHANGGuan-nan ,  XIAOYi , QIUHui-zhong

Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China;

Corresponding author：

XIAOYi, Email: xiaoy@pumch.cn

Keywords：

miR-338-5p; Colorectal cancer; Cell proliferation; Cell apoptosis; Cell cycle

DOI：

10.7507/1007-9424.20140145

Video：

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Objective To evaluate the expression of miR-338-5p in colorectal cancer tissues and study its role in colon cancer cell proliferation, apoptosis, and cell cycle. Methods The expression of miR-338-5p was detected by real-time PCR in the colorectal cancer tissues and corresponding adjacent to cancer tissue samples. The miR-338-5p-mimics was transfected into the colon cancer cell lines HCT116 and SW620 to investigate its role in cell proliferation, apoptosis, and cell cycle. The cell proliferation and apoptosis were measured by CCK-8 and flow cytometry, respectively. The cell cycle was also analyzed by flow cytometry. Results ①miR-338-5p expression was significantly downregulated in the colorectal cancer tissues as compared with corresponding adjacent to cancer tissue samples(P < 0.01). 2 Compared with the transfected negative control cells, the proliferation ability of colon cancer cell HCT116 or SW620 was significantly decreased(P < 0.01), cell apoptosis was significantly increased[HCT116 cell:(11.43±0.67)% versus(7.98±0.36)%, P < 0.01;SW620 cell:(10.5±0.2)% versus(7.93±0.5)%, P < 0.01), and cell G₁ was arrested[HCT116 cell:(80.41±1.34)% versus (64.87±1.83)%, P < 0.01;SW620 cell:(68.76±0.41)% versus(54.89±0.78)%, P < 0.01) after transfecting miR-338-5p-mimics cells. Conclusion miR-338-5p may act as an anti-oncogene in colorectal cancer through regulation of cell proliferation, apoptosis, and cell cycle.

Citation： XIONGGuang-bing, ZHANGGuan-nan, XIAOYi, QIUHui-zhong. miR-338-5p Is Downregulated in Colorectal Cancer and Its Role in Inhibiting Colon Cancer Cell Proliferation. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(5): 603-608. doi: 10.7507/1007-9424.20140145 Copy

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1. Cunningham D, Atkin W, Lenz HJ, et al.Colorectal cancer[J].Lancet, 2010, 375(9719):1030-1047.
2. Jemal A, Bray F, Center MM, et al.Global cancer statistics[J].CA Cancer J Clin, 2011, 61(2):69-90.
3. Fearon ER.Molecular genetics of colorectal cancer[J].Annu Rev Pathol, 2011, 6:479-507.
4. Venkatachalam R, Ligtenberg MJ, Hoogerbrugge N, et al.The epigenetics of (hereditary) colorectal cancer[J].Cancer Genet Cytogenet, 2010, 203(1):1-6.
5. Bartel DP.MicroRNAs:genomics, biogenesis, mechanism, and function[J].Cell, 2004, 116(2):281-297.
6. Carthew RW, Sontheimer EJ.Origins and mechanisms of miRNAs and siRNAs[J].Cell, 2009, 136(4):642-655.
7. Calin G, Croce CM.MicroRNA signatures in human cancers[J].Nat Rev Cancer, 2006, 6(11):857-866.
8. Lee YS, Dutta A.MicroRNAs in cancer[J].Annu Rev Pathol, 2009, 4:199-227.
9. Place RF, Li LC, Pookot D, et al.MicroRNA-373 induces expression of genes with complementary promoter sequences[J].Proc Natl Acad Sci U S A, 2008, 105(5):1608-1613.
10. Huang V, Li LC.miRNA goes nuclear[J].RNA Biol, 2012, 9(3):269-273.
11. Xue Q, Sun K, Deng HJ, et al.MicroRNA-338-3p inhibits colorectal carcinoma cell invasion and migration by targeting smoothened[J].Jpn J Clin Oncol, 2014, 44(1):13-21.
12. Sun K, Deng HJ, Lei ST, et al.miRNA-338-3p suppresses cell growth of human colorectal carcinoma by targeting smoothened[J].World J Gastroenterol, 2013, 19(14):2197-2207.
13. Shenouda SK, Alahari SK.MicroRNA function in cancer:oncogene or a tumor suppressor?[J].Cancer Metastasis Rev, 2009, 28(3/4):369-378.
14. Toiyama Y, Hur K, Tanaka K, et al.Serum miR-200c is a novel prognostic and metastasis-predictive biomarker in patients with colorectal cancer[J].Ann Surg, 2014, 259(4):735-743.
15. Zhang JX, Song W, Chen ZH, et al.Prognostic and predictive value of a microRNA signature in stageⅡcolon cancer:a micro-RNA expression analysis[J].Lancet Oncol, 2013, 14(13):1295-1306.
16. Dugas JC, Cuellar TL, Scholze A, et al.Dicer1 and miR-219 are required for normal oligodendrocyte differentiation and myelination[J].Neuron, 2010, 65(5):597-611.
17. Aschrafi A, Kar AN, Natera-Naranjo O, et al.MicroRNA-338 regulates the axonal expression of multiple nuclear-encoded mitochondrial mRNAs encoding subunits of the oxidative phosphorylation machinery[J].Cell Mol Life Sci, 2012 Jul 8.[Epub ahead of print].
18. Li P, Chen X, Su L, et al.Epigenetic silencing of miR-338-3p contributes to tumorigenicity in gastric cancer by targeting SSX2IP[J].PLoS One, 2013, 8(6):e66782.
19. Guo B, Liu L, Yao J, et al.miR-338-3p suppresses progression of gastric cancer through PTEN-AKT signaling pathways by targeting P-REX2a[J].Mol Cancer Res, December 27, 2013.
20. Huang XH, Wang Q, Chen JS, et al.Bead-based microarray analysis of microRNA expression in hepatocellular carcinoma:miR-338 is downregulated[J].Hepatol Res, 2009, 39(8):786-794.
21. Fu X, Tan D, Hou Z, et al.The effect of miR-338-3p on HBx deletion-mutant (HBx-d382) mediated liver-cell proliferation through CyclinD1 regulation[J].PLoS One, 2012, 7(8):e43204.
22. Wu B, Li C, Zhang P, et al.Dissection of miRNA-miRNA interaction in esophageal squamous cell carcinoma[J].PLoS One, 2013, 8(9):e73191.
23. Yang M, Liu R, Sheng J, et al.Differential expression profiles of microRNAs as potential biomarkers for the early diagnosis of esophageal squamous cell carcinoma[J].Oncol Rep, 2013, 29(1):169-176.
24. Chen X, Pan M, Han L, et al.miR-338-3p suppresses neuroblastoma proliferation, invasion and migration through targeting PREX2a[J].FEBS Lett, 2013, 587(22):3729-3737.
25. Yu J, Li A, Hong SM, et al.MicroRNA alterations of pancreatic intraepithelial neoplasias[J].Clin Cancer Res, 2012, 18(4):981-992.
26. Cheung TH, Man KN, Yu MY, et al.Dysregulated microRNAs in the pathogenesis and progression of cervical neoplasm[J].Cell Cycle, 2012, 11(15):2876-2884.
27. Maclellan S, Lawson J, Baik J, et al.Differential expression of miRNAs in the serum of patients with high-risk oral lesions[J].Cancer Med, 2012, 1(2):268-274.
28. Won KY, Kim YW, Kim HS, et al.MicroRNA-199b-5p is involved in the Notch signaling pathway in osteosarcoma[J].Hum Pathol, 2013, 44(8):1648-1655.
29. Schetter AJ, Leung SY, Sohn JJ, et al.MicroRNA expression profiles associated with prognosis and therapeutic outcome in colon adenocarcinoma[J].JAMA, 2008, 299(4):425-436.
30. Gaedcke J, Grade M, Camps J, et al.The rectal cancer micro-RNAome-microRNA expression in rectal cancer and matched normal mucosa[J].Clin Cancer Res, 2012, 18(18):4919-4930.
31. Yong FL, Law CW, Wang CW.Potentiality of a triple microRNA classifier:miR-193a-3p, miR-23a and miR-338-5p for early detection of colorectal cancer[J].BMC Cancer, 2013, 13:280.
32. Fu X, Tan D, Hou Z, et al.miR-338-3p is down-regulated by hepatitis B virus X and inhibits cell proliferation by targeting the 3'-UTR region of cyclinD1[J].Int J Mol Sci, 2012, 13(7):8514-8539.

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miR-338-5p Is Downregulated in Colorectal Cancer and Its Role in Inhibiting Colon Cancer Cell Proliferation

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