Establishment and Evaluation of Type 2 Diabetes SD Rat Model_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 SHAOJun-wei ,  CAIXun , MADan-dan , HUYi-lin , ZHANGYi

Department of General Surgery, Wuhan General Hospital of Guangzhou Command, Wuhan 430070, Hubei Province, China;

Corresponding author：

CAIXun, Email: caiwenqian@sina.com

Keywords：

Type 2 diabetes; Rat model; Streptozotocin; High fat and glucose diet

DOI：

10.7507/1007-9424.20140291

Video：

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Objective To evaluate the influencing factors and explore a better method of making rat model of type 2 diabetes by high fat and sugar diet and streptozotocin(STZ) injection. Methods Sixty SPF grade of 6 weeks male SD rats were fed with high fat and glucose diet by 4 weeks and then randomly divided into 3 groups, the control group rats(n=20) were injected citric acid by 50 mg/kg and fed with normal diet, and the diabetes mellitus group rats were further divided into 2 subgroups by the different doses of STZ:the rats of diabetes mellitus model group 1(n=20) were injected by 50 mg/kg, while the rats of diabetes mellitus model group 2(n=20) were injected by 35 mg/kg. The diabetes mellitus model group rats were fed with high fat and glucose diet continually. The fasting blood glucose(FBG) were measured on day 3, 7, 10, and 14, respectively. The success model rate(blood glucose > 16.7 mmol/L after 14 days) and the mortality rate were calculated. Meanwhile fasting serum insulin level(FSI), total serum cholesterol(TC), and triglyceride(TG) were measured. Results Compared with the control group, the levels of FBG, FSI, TC, and TG were increased significantly in the diabetes mellitus model group 1 and 2(P < 0.05). And insulin sensitivity was worsen markedly(P < 0.05). But the diabetes mellitus model group 2 had higher success rate of making model(85% vs. 75%) and lower mortality(0 vs. 25%), P < 0.05. Conclusion Rat model of type 2 diabetes induced by 4 weeks of high fat and sugar feeding and 35 mg/kg STZ injection has high morbidity, strongly security, and stable features.

Citation： SHAOJun-wei, CAIXun, MADan-dan, HUYi-lin, ZHANGYi. Establishment and Evaluation of Type 2 Diabetes SD Rat Model. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(10): 1212-1215. doi: 10.7507/1007-9424.20140291 Copy

1.	Wild S, Roglic G, Green A, et al. Global prevalence of diabetes:estimates for the year 2000 and projections for 2030[J]. Diabetes Care, 2004, 27(5):1047-1053.
2.	Expert Committee on the Diagnosis and Classification of Diabetes Mellitus. Report of the expert committee on the diagnosis and classification of diabetes mellitus[J]. Diabetes Care, 2003, 26 Suppl 1:S5-S20.
3.	杨架林, 李果, 刘优萍, 等.长期高脂饮食加小剂量链脲佐菌素建立人类普通2型糖尿病大鼠模型的研究[J].中国实验动物学报, 2003, 11(3):138-141.
4.	郭啸华, 刘志红, 李恒, 等.高糖高脂饮食诱导的2型糖尿病大鼠模型及其肾病特点[J].中国糖尿病杂志, 2002, 10(5):290-294.
5.	杨李, 唐瑛, 陈芳, 等.芪莲汤颗粒长期干预对大鼠糖尿病并发症的影响[J].医药导报, 2011, 30(5):563-565.
6.	张玉领, 陈培, 刘家秀, 等. 2型糖尿病大鼠模型的建立[J].现代预防医学, 2012, 39(15):3922-3924.
7.	汪园园, 欧斌, 李苏华, 等.利用高糖高脂饮食建立胰岛素抵抗大鼠模型[J].深圳中西医结合杂志, 2011, 21(1):13-14.
8.	Goto Y, Kakizaki M, Masaki N. Production of spontaneous diabetic rats by repetition of selective breeding[J]. Tohoku J Exp Med, 1976, 119(1):85-90.
9.	Srinivasan K, Ramarao P. Anamal models in type 2 diabetes research:an overview[J]. Indian J Med Res, 2007, 125(3):451-472.
10.	Al-Khalifa A, Mathew TC, Al-Zaid NS, et al. Low carbohydrate ketogenic diet prevents the induction of diabetes using streptozotocin in rats[J]. Exp Toxicol Pathol, 2011, 63(7-8):663-669.
11.	Kim CH, Youn JH, Park JY, et al. Effects of high-fat diet and exercise training on intracellular glucose metabolism in rats[J]. Am J Physiol Endo Metab, 2000, 278(6):977-984.
12.	张均田主编.现代药理学实验方法[M].北京:北京医科大学中国协和医科大学联合出版社, 1998:981-988.
13.	沈亚非, 徐焱成.链脲佐菌素诱导实验性糖尿病模型建立的研究[J].实用诊断与治疗杂志, 2005, 19(2):79-80.
14.	Winzell MS, Ahrén B. The high-fat diet-fed mouse:a model for studying mechanisms and treatment of impaired glucose tolerance and type 2 diabetes[J]. Diabetes, 2004, 53 Suppl 3:S215-S219.
15.	Chalkley SM, Hettiarachchi M, Chisholm DJ, et al. Long-term high-fat feeding leads to severe insulin resistance but not diabetes in Wistar rats[J]. Am J Physiol Endocrinol Metab, 2002, 282(6):1231-1238.
16.	董佳生, 徐华, 王露萍, 等. SD大鼠I型糖尿病动物模型的建立[J].山西医药杂志, 2009, 38(3):218-220.
17.	Wilkinson-Berka JL, Kelly DJ, Koerner SM, et al. ALT-946 and aminoguanidine, inhibitors of advanced glycation, improve seven nephropathyin the diabetic transgenic (mREN-2) 27 rats[J]. Diabetes, 2002, 51(11):3283-3289.
18.	孙兆峰, 王利, 夏作理. 2型糖尿病动物模型研究概要[J].中国微循环, 2008, 12(3):187-189.
19.	Zhou YS, Gao Y, Guo XH, et al. Effect of timely insulin treatment on protection ofβcells in a rat model of type 2 diabetes mellitus[J]. Chin Med J (Engl), 2004, 117(10):1523-1529.
20.	施红, 金国琴, 余文珍.诱导构建最佳类似人类2型糖尿病大鼠的造模方式[J].中国临床康复, 2005, 9(39):69-71.
21.	胡玉焕, 宁亚功, 肖燕.高热量饮食联合链脲佐菌素建立2型糖尿病大鼠模型的影响因素[J].江西中医学院学报, 2013, 25(1):70-73.
22.	Morimoto S, Mendoza-Rodriguez CA, Hiriart M, et al. Protective effect of testosterone on early apoptotic damage induced by streptozotocin in rat pancreas[J]. J Endocrinol, 2005, 187(2):217-224.
23.	蒋升, 谢自敬, 张莉.链脲佐菌素诱导1型糖尿病大鼠模型稳定性观察[J].中国比较医学杂志, 2006, 16(1):16-18.
24.	Masiello P, De Paoli A, Bergamini E. Age-dependent changes in the sensitivity of the rat to a diabetoenic agent (streptozotocin)[J]. Endocrinology, 1975, 96(3):787-789.

1. Wild S, Roglic G, Green A, et al. Global prevalence of diabetes:estimates for the year 2000 and projections for 2030[J]. Diabetes Care, 2004, 27(5):1047-1053.
2. Expert Committee on the Diagnosis and Classification of Diabetes Mellitus. Report of the expert committee on the diagnosis and classification of diabetes mellitus[J]. Diabetes Care, 2003, 26 Suppl 1:S5-S20.
3. 杨架林, 李果, 刘优萍, 等.长期高脂饮食加小剂量链脲佐菌素建立人类普通2型糖尿病大鼠模型的研究[J].中国实验动物学报, 2003, 11(3):138-141.
4. 郭啸华, 刘志红, 李恒, 等.高糖高脂饮食诱导的2型糖尿病大鼠模型及其肾病特点[J].中国糖尿病杂志, 2002, 10(5):290-294.
5. 杨李, 唐瑛, 陈芳, 等.芪莲汤颗粒长期干预对大鼠糖尿病并发症的影响[J].医药导报, 2011, 30(5):563-565.
6. 张玉领, 陈培, 刘家秀, 等. 2型糖尿病大鼠模型的建立[J].现代预防医学, 2012, 39(15):3922-3924.
7. 汪园园, 欧斌, 李苏华, 等.利用高糖高脂饮食建立胰岛素抵抗大鼠模型[J].深圳中西医结合杂志, 2011, 21(1):13-14.
8. Goto Y, Kakizaki M, Masaki N. Production of spontaneous diabetic rats by repetition of selective breeding[J]. Tohoku J Exp Med, 1976, 119(1):85-90.
9. Srinivasan K, Ramarao P. Anamal models in type 2 diabetes research:an overview[J]. Indian J Med Res, 2007, 125(3):451-472.
10. Al-Khalifa A, Mathew TC, Al-Zaid NS, et al. Low carbohydrate ketogenic diet prevents the induction of diabetes using streptozotocin in rats[J]. Exp Toxicol Pathol, 2011, 63(7-8):663-669.
11. Kim CH, Youn JH, Park JY, et al. Effects of high-fat diet and exercise training on intracellular glucose metabolism in rats[J]. Am J Physiol Endo Metab, 2000, 278(6):977-984.
12. 张均田主编.现代药理学实验方法[M].北京:北京医科大学中国协和医科大学联合出版社, 1998:981-988.
13. 沈亚非, 徐焱成.链脲佐菌素诱导实验性糖尿病模型建立的研究[J].实用诊断与治疗杂志, 2005, 19(2):79-80.
14. Winzell MS, Ahrén B. The high-fat diet-fed mouse:a model for studying mechanisms and treatment of impaired glucose tolerance and type 2 diabetes[J]. Diabetes, 2004, 53 Suppl 3:S215-S219.
15. Chalkley SM, Hettiarachchi M, Chisholm DJ, et al. Long-term high-fat feeding leads to severe insulin resistance but not diabetes in Wistar rats[J]. Am J Physiol Endocrinol Metab, 2002, 282(6):1231-1238.
16. 董佳生, 徐华, 王露萍, 等. SD大鼠I型糖尿病动物模型的建立[J].山西医药杂志, 2009, 38(3):218-220.
17. Wilkinson-Berka JL, Kelly DJ, Koerner SM, et al. ALT-946 and aminoguanidine, inhibitors of advanced glycation, improve seven nephropathyin the diabetic transgenic (mREN-2) 27 rats[J]. Diabetes, 2002, 51(11):3283-3289.
18. 孙兆峰, 王利, 夏作理. 2型糖尿病动物模型研究概要[J].中国微循环, 2008, 12(3):187-189.
19. Zhou YS, Gao Y, Guo XH, et al. Effect of timely insulin treatment on protection ofβcells in a rat model of type 2 diabetes mellitus[J]. Chin Med J (Engl), 2004, 117(10):1523-1529.
20. 施红, 金国琴, 余文珍.诱导构建最佳类似人类2型糖尿病大鼠的造模方式[J].中国临床康复, 2005, 9(39):69-71.
21. 胡玉焕, 宁亚功, 肖燕.高热量饮食联合链脲佐菌素建立2型糖尿病大鼠模型的影响因素[J].江西中医学院学报, 2013, 25(1):70-73.
22. Morimoto S, Mendoza-Rodriguez CA, Hiriart M, et al. Protective effect of testosterone on early apoptotic damage induced by streptozotocin in rat pancreas[J]. J Endocrinol, 2005, 187(2):217-224.
23. 蒋升, 谢自敬, 张莉.链脲佐菌素诱导1型糖尿病大鼠模型稳定性观察[J].中国比较医学杂志, 2006, 16(1):16-18.
24. Masiello P, De Paoli A, Bergamini E. Age-dependent changes in the sensitivity of the rat to a diabetoenic agent (streptozotocin)[J]. Endocrinology, 1975, 96(3):787-789.

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Establishment and Evaluation of Type 2 Diabetes SD Rat Model

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