Animal Experiment on Influence of Different Radiation Doses of <sup>125</sup>ⅠSeed on Human Poorly Differentiated Gastric Cancer Cells_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 LIXiao-gang ¹ , LUPing ² , JINWen-di ¹ , LIBo ¹ , WANGChun-xiao ¹ , NIUChao ¹ ,  LUOKai-yuan ¹

1. Department of General Surgery, The Fourth Affiliated Hospital, Kunming Medical University, Abdominal Minimally Invasive Surgery Research Center of Yunnan Province, Kunming 650021, Yunnan Province, China;
2. Department of Vascular Surgery, The Fourth Affiliated Hospital, Kunming Medical University, Kunming 650021, Yunnan Province, China;

Corresponding author：

LUOKai-yuan, Email: ynluoky@aliyun.com

Keywords：

¹²⁵Ⅰseed; Human poorly differentiated gastric cancer cell; Cell apoptosis; CyclinE

DOI：

10.7507/1007-9424.20140359

Video：

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Objective To explore the influence of different radiation doses of ¹²⁵Ⅰseed on human poorly differentiated gastric cancer cells (BGC823). Methods Sixty four male nude mice of BLAB nu/nu inoculated with human poorly differentiated gastric cancer cells (BGC823) were took as animal models. When tumors of nude mice grew to 0.7-1.2 cm, the nude mice were randomly divided into blank control group, low dose group, middle dose group, and high dose group (n=16). The tumors of nude mice in blank control group were implanted with blank seed, but tumors of nude mice in low dose group, middle dose group, and high dose group were implanted with ¹²⁵Ⅰseed (the radiation doses of 3 groups were 1.48×10^-7 Bq, 2.22×10^-7 Bq, and 2.96×10^-7 Bq respectively). Four nude mice of 4 groups were randomly collected to measure the tumor volume and weight before implantation, and on 7, 14, 21, and 28 days after implantation, but apoptosis rates and expression levels of cyclinE mRNA were measured on 14 days and 28 days after implantation, by using flow cytometer and semi quantitative RT-PCR method respectively. Results ①Except for the blank control group, the tumor volume and weight decreased over time in low dose group, middle dose group, and high dose group. The tumor volume and weight in blank control group were higher than those of other 3 groups on 7, 14, 21, and 28 days after implantation (P < 0.05);in addition, on 28 days after implantation, tumor volume and weight in low dose group were lower than those of middle dose group and high dose group (P < 0.05).②On 14 days and 28 days after implantation, the apoptosis rates of blank control group were lower than those of other 3 groups (P < 0.05), but expression levels of cyclinE mRNA were all higher than those of other 3 groups (P < 0.05). In addition, on 28 days after implantation, apoptosis rate of low dose group was higher than both of middle dose group and high dose group (P < 0.05), but expression level of cyclinE mRNA was lower (P < 0.05). Compared with 14 days in the same group, except for the blank control group (P > 0.05), the apoptosis rates in other 3 groups on 28 days were higher (P < 0.05), with the lower expression levels of cyclinE mRNA (P < 0.05). Conclusions ¹²⁵Ⅰseed in organizational implantation can effectively inhibit the expression of cyclinE mRNA and the growth of human poorly differentiated gastric cancer tissue. Compared with doses of 2.22×10^-7 Bq and 2.96×10^-7 Bq of ¹²⁵Ⅰ, low dose (1.48×10^-7 Bq) contributes to the apoptosis of human poorly differentiated gastric cancer cells (BGC823).

Citation： LIXiao-gang, LUPing, JINWen-di, LIBo, WANGChun-xiao, NIUChao, LUOKai-yuan. Animal Experiment on Influence of Different Radiation Doses of ¹²⁵ⅠSeed on Human Poorly Differentiated Gastric Cancer Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(12): 1518-1523. doi: 10.7507/1007-9424.20140359 Copy

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1. 张文范.消化系统癌症手术与综合治疗[M].沈阳:辽宁科学技术出版社, 1999:418-419.
2. Di Betta E, D'hoore A, Filez L, et al.Sphincter saving rectum resection is the standard procedure for low rectal cancer[J].Int J Colorectal Dis, 2003, 18(6):463-469.
3. Saito N, Sugito M, Ito M, et al.Oncologic outcome of intersphincteric resection for very low rectal cancer[J].World J Surg, 2009, 33(8):1750-1756.
4. Koff A, Gordano A, Desai D, et al.Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle[J].Science, 1992, 257(5077):1689-1694.
5. 贾永蕊.药学实验室技术系列流式细胞术[M].北京:化学工业出版社, 2009:1-3.
6. 罗开元.实用组织间植入内放射治疗恶性肿瘤学[M].北京:人民卫生出版社, 2008:9-16.
7. Kroger LA, Denardo GL, Gumerlock PH, et al.Apoptosis-related gene and protein expression in human lymphoma xenografts (Raji) after low dose rate radiation using 67Cu-2IT-BAT-Lym-1 radioimmunotherapy[J].Cancer Biother Radiopharm, 2001, 16(3):213-225.
8. Herbst RS.Review of epidermal growth factor receptor biology[J].Int J Radiat Oncol Biol Phys, 2004, 59(2 Suppl):21-26.
9. 杨嵘, 陈亿, 罗开元.¹²⁵Ⅰ粒子对人结肠腺癌裸鼠皮下移植瘤的敏感性研究[J].实用癌症杂志, 2010, 25(3):221-224.
10. Yan L, Beckman RA.Pharmacogenetics and pharmacogenomics in oncology therapeutic antibody development[J].Biotechniques, 2005, 39(4):565-568.
11. Shimamura H, Sunamura M, Tsuchihara K, et al.Irradiated pancreatic cancer cells undergo both apoptosis and necrosis, and could be phagocytized by dendritic cells[J].Eur Surg Res, 2005, 37(4):228-234.
12. Adams J, Carder PJ, Downey S, et al.Vascular endothelial growth factor (VEGF) in breast cancer:comparison of plasma, serum, and tissue VEGF and microvessel density and effects of tamoxifen[J].Cancer Res, 2000, 60(11):2898-2905.
13. Kerr IF, Wyllie AH, Currie AR.Apoptosis:a basic biological phenomenon with wide-ranging implications in tissue kinetics[J].Cancer, 1972, 26(4):239-257.
14. Tinel A, Tschopp J.The PIDDosome, a protein complex implic-ated in activation of caspase-2 in response to genotoxic stress[J].Science, 2004, 304(5672):843-846.
15. Kastan MB, Zhan Q, el-Deiry WS, et al.A mammalian cell cycle checkpoint pathway utilizing p53 and GADD45 is defective in ataxia-telangiectasia[J].Cell, 1992, 71(4):587-597.
16. Resnitzky D, Gossen M, Bujard H, et al.Acceleration of the G1/S phase transition by expression of cyclins D1 and E with an inducible system[J].Mol Cell Biol, 1994, 14(3):1669-1679.
17. Polyak K, Kato JY, Solomon MJ, et al.p27kip1, a cyclin-Cdk inhibitor, links transforming growth factor-beta and contact inhibition to cell cycle arrest[J].Genes Dev, 1994, 8(1):9-22.
18. 季加孚.胃癌外科的现状与发展趋势[J].中国普外基础与临床杂志, 2006, 13(1):1-3.
19. 陈曦海, 张岂凡, 马荣.反义survivin诱导胃癌SGC7901细胞凋亡及逆转耐药的研究[J].中国普外基础与临床杂志, 2007, 14(1):63-67.
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21. Zhang H, Berezov A, Wang Q, et al.ErbB receptors:from oncogenes to targeted cancer therapies[J].J Clin Invest, 2007, 117(8):2051-2058.
22. 李小军, 张清华, 罗婷, 等.胃癌组织中过氧化物酶增殖因子活化受体与VEGF mRNA表达的相关性和意义[J].中国普外基础与临床杂志, 2008, 15(7):498-502.
23. Motyl T.Regulation of apoptosis:involvement of Bcl-2-related proteins[J].Reprod Nutr Dev, 1999, 39(1):49-59.
24. 陆平, 杨镛, 李晓刚, 等.¹²⁵Ⅰ粒子组织间植入抑制乳腺癌生长的实验研究[J].中国普外基础与临床杂志, 2009, 16(7):510-513.
25. 李滢旭, 罗开元, 陈亿, 等.¹²⁵Ⅰ粒子抑制中分化结肠腺癌的实验研究[J].中国普外基础与临床杂志, 2010, 17(10):1062-1066.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Animal Experiment on Influence of Different Radiation Doses of ¹²⁵ⅠSeed on Human Poorly Differentiated Gastric Cancer Cells

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Animal Experiment on Influence of Different Radiation Doses of 125ⅠSeed on Human Poorly Differentiated Gastric Cancer Cells

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Animal Experiment on Influence of Different Radiation Doses of ¹²⁵ⅠSeed on Human Poorly Differentiated Gastric Cancer Cells