Twist与E-cadherin在结直肠癌中的表达及其意义_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 钱江 , 韩佳 , 陈鹏 , 朱春丽 , 杨银

重庆市垫江县中医院普外科（重庆垫江 408300）;

Corresponding author：

钱江, Email: qianjiang688@126.com

Keywords：

结直肠癌; Twist蛋白; E-钙粘素; 免疫组织化学

DOI：

10.7507/1007-9424.20150031

Video：

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目的探讨Twist和E-cadherin蛋白在结直肠癌组织中的表达及其可能的机理、相互作用以及与结直肠癌预后的关系。方法应用免疫组化SP法检测63例结直肠癌组织以及21例正常结肠黏膜组织中Twist和E-cadherin蛋白的表达，并分析其与结直肠癌临床病理特征和患者预后的关系。结果 Twist蛋白在结直肠癌组织中的表达阳性率为66.7%（42/63），而在正常结直肠黏膜组织中的表达阳性率为14.2%（3/21），前者高于后者（P＜0.01）；E-cadherin蛋白在结直肠癌组织中的表达阳性率为20.6%（13/63），而在正常结直肠黏膜组织中的表达阳性率为81.0%（17/21），前者低于后者（P＜0.01）。两者的表达与肿瘤的分化程度及T、N和M分期均相关（P＜0.05）。相关性分析显示，Twist和E-cadherin蛋白的表达呈负相关关系（r=-0.215，P=0.039）。结论 Twist和E-cadherin蛋白的表达可能与结直肠癌的发生发展和侵袭转移相关，并可作为评价结直肠癌生物学行为和预后的重要指标。Twist和E-cadherin蛋白表达呈负相关关系提示，在上皮-间质转化过程中可能是通过抑制E-cadherin蛋白的表达来实现的。

Citation： 钱江, 韩佳, 陈鹏, 朱春丽, 杨银. Twist与E-cadherin在结直肠癌中的表达及其意义. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(1): 114-116. doi: 10.7507/1007-9424.20150031 Copy

1.	Thiery JP, Sleeman JP.Complex networks orchestrate epithelial-mesenchymal transitions.Nat Rev Mol Cell Biol, 2006, 7(2):131-142.
2.	Peinado H, Olmeda D, Cano A.Snail, Zeb and bHLH factors intumour progression:an alliance against the epithelial phenotype? NatRev Cancer, 2007, 7(6):415-428.
3.	Kyo S, Sakaguchi J, Ohno S, et al.High Twist expression is involvedin infiltrative endometrial cancer and affects patient survival.Hum Pathol, 2006, 37(4):431-438.
4.	Zhang Z1, Xie D, Li X, et al.Significance of TWIST expression and its association with E-cadherin in bladder cancer.Hum Pathol.2007, 38(4):598-606.
5.	Lee TK, Poon RT, Yuen AP, et al.Twist overexpression correlates with hepatocellular carcinoma metastasis through induction of epithelial-mesenchymal transition.Clin Cancer Res, 2006, 12(18):5369-5376.
6.	Yang Z, Zhang X, Gang H, et al.Up-regulation of gastric cancer cell invasion by Twist is accompanied by N-cadherin and fibronectin expression.Biochem Biophys Res Commun, 2007, 358(3):925-930.
7.	Early DS, Fontana L, Davidson NO.Translational approaches to addressing complex genetic pathways in colorectal cancer.Transl Res, 2008, 151(1):10-16.
8.	Lee S, Bang S, Song K, et al.Differential expression innormal adenoma carcinoma sequence suggests complex molecular carcinogenesis in colon.Oncol Rep, 2006, 16(4):747-754.
9.	Castanon I, Baylies MK.A Twist in fate:evolutionary comparison of TWIST structure and function.Gene, 2002, 287(1-2):11-22.
10.	Zuniga A, Quillet R, Perrin-Schmitt F, et al.Mouse Twist is required for fibroblast growth factor-mediated epithelial-mesenchymal signalling and cell survival during limb morphogenesis.Mech Dev, 2002, 114(1-2):51-59.
11.	Frackler MJ, McVeigh M, Evron E, et al.DNA methylation of RASSFIA, HIN-1, RAR-beta, Cyclin D2 and Twist in in situ and invasive lobular breast carcinoma.Int J Cancer, 2003, 107(6):970-975.
12.	Mehrota J, Vali M, McVeigh M et al.Very high frequency of hypermethylated genes in breast cancer metastasis to the bone, brain, and lung.Clin Cancer Res, 2004, 10(9):3104-3109.
13.	Yang MH, Wu MZ, Chiou SH, et al.Direct regulation of TWIST by HIF-1alpha promotes metastasis.Nat Cell Biol, 2008, 10(3):295-305.

1. Thiery JP, Sleeman JP.Complex networks orchestrate epithelial-mesenchymal transitions.Nat Rev Mol Cell Biol, 2006, 7(2):131-142.
2. Peinado H, Olmeda D, Cano A.Snail, Zeb and bHLH factors intumour progression:an alliance against the epithelial phenotype? NatRev Cancer, 2007, 7(6):415-428.
3. Kyo S, Sakaguchi J, Ohno S, et al.High Twist expression is involvedin infiltrative endometrial cancer and affects patient survival.Hum Pathol, 2006, 37(4):431-438.
4. Zhang Z1, Xie D, Li X, et al.Significance of TWIST expression and its association with E-cadherin in bladder cancer.Hum Pathol.2007, 38(4):598-606.
5. Lee TK, Poon RT, Yuen AP, et al.Twist overexpression correlates with hepatocellular carcinoma metastasis through induction of epithelial-mesenchymal transition.Clin Cancer Res, 2006, 12(18):5369-5376.
6. Yang Z, Zhang X, Gang H, et al.Up-regulation of gastric cancer cell invasion by Twist is accompanied by N-cadherin and fibronectin expression.Biochem Biophys Res Commun, 2007, 358(3):925-930.
7. Early DS, Fontana L, Davidson NO.Translational approaches to addressing complex genetic pathways in colorectal cancer.Transl Res, 2008, 151(1):10-16.
8. Lee S, Bang S, Song K, et al.Differential expression innormal adenoma carcinoma sequence suggests complex molecular carcinogenesis in colon.Oncol Rep, 2006, 16(4):747-754.
9. Castanon I, Baylies MK.A Twist in fate:evolutionary comparison of TWIST structure and function.Gene, 2002, 287(1-2):11-22.
10. Zuniga A, Quillet R, Perrin-Schmitt F, et al.Mouse Twist is required for fibroblast growth factor-mediated epithelial-mesenchymal signalling and cell survival during limb morphogenesis.Mech Dev, 2002, 114(1-2):51-59.
11. Frackler MJ, McVeigh M, Evron E, et al.DNA methylation of RASSFIA, HIN-1, RAR-beta, Cyclin D2 and Twist in in situ and invasive lobular breast carcinoma.Int J Cancer, 2003, 107(6):970-975.
12. Mehrota J, Vali M, McVeigh M et al.Very high frequency of hypermethylated genes in breast cancer metastasis to the bone, brain, and lung.Clin Cancer Res, 2004, 10(9):3104-3109.
13. Yang MH, Wu MZ, Chiou SH, et al.Direct regulation of TWIST by HIF-1alpha promotes metastasis.Nat Cell Biol, 2008, 10(3):295-305.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Twist与E-cadherin在结直肠癌中的表达及其意义

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