Changes of Peripheral Blood Lymphocyte Subsets of Patients with Primary Hepatocellular Carcinoma Treated with Radiofrequency Ablation_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 LIUJiang-wei ,  HUANGJian-zhao , SUNQian , GOU-xin , LIUYan , ZHAOPeng-wei , ZHANGJia-wei

Department of Hepatobiliary Surgery, Guizhou Provincial People, s Hospita, Guiyang 550002, Guizhou Province, China;

Corresponding author：

HUANGJian-zhao, Email: huangjzgz@126.com

Keywords：

Primary hepatocellular carcinoma; Radiofrequency ablation; Lymphocyte subsets

DOI：

10.7507/1007-9424.20150305

Video：

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Objective To observe the changes of peripheral blood lymphocyte subsets of patients with primary hepatocellular carcinoma (PHCC) treated with radiofrequency ablation (RFA). Methods The data of 70 cases of hospitalized patients with PHCC that voluntary accepted RFA diagnosed by clinical and pathological in our hospital between July 2011 and December 2014 were collected. According to the numbers of HCC lesions, 70 cases were divided into single focus group (n=41) and multifocal group (n=29). The changes of their immune parameters before and after RFA were analyzed. Results ①The ratioes of peripheral blood CD3⁺/CD19^-, CD3⁺/CD4⁺, CD4⁺/CD8⁺, and NK cells on 7 days and 14 days after RFA treatment of 70 cases were significantly higher than those on 1 day before RFA treatment (P < 0.05). The ratio of CD3⁺/CD8⁺ T cells reduced from 1 day before RFA treatment to 14 days after RFA treatment, but the difference was no statistically significant (P > 0.05).②The changing trend of peripheral blood lymphocyte subsets before and after RFA treatment in single focus group and multifocal group were similar to the above.③Compared with single focus group, the ratioes of peripheral blood CD3⁺/CD19^-, CD3⁺/CD4⁺, CD4⁺/CD8⁺, and NK cells before and after RFA treatment in multifocal group were lower, and the ratio of CD3⁺/CD8⁺ T cells was higher, but the difference were not statistically significant (P > 0.05). Conclusion RFA can not only destroy small PHCC foci, but also to significantly improve immune function and enhance the anti-tumor effect.

Citation： LIUJiang-wei, HUANGJian-zhao, SUNQian, GOU-xin, LIUYan, ZHAOPeng-wei, ZHANGJia-wei. Changes of Peripheral Blood Lymphocyte Subsets of Patients with Primary Hepatocellular Carcinoma Treated with Radiofrequency Ablation. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(10): 1171-1174. doi: 10.7507/1007-9424.20150305 Copy

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7.	Buscarini E, Savoia A, Brambilla G, et al. Radiofrequency thermal ablation of liver tumors. Eur Radiol, 2010, 15(5):884-894.
8.	Truk MJ, Guevara-Patiño JA, Rizzuto GA, et al. Concomitant tumor immunity to a poorly immunogenic melanoma is prevented by regulatory T cells. J Exp Med, 2011, 200(6):771-782.
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12.	Liu Y, Poon RT, Hughes J, et al. Chemokine receptions support infiltration of lymphocyte subpopulations in human hepatocelluar carcinoma. Clin Immunol, 2005, 114(2):174-182.
13.	Bentdal O, FrØland SS, Bosnes V, et al. Alteration in lymphocyte subsets in blood may predict resectibility in carcinoma of cardia or oesophogus. Cancer Letters, 1996, 100(1-2):133-138.
14.	周永昌, 郭万学.超声医学.第4版.北京:北京科学技术文献出版社, 2003:1622-1624.
15.	陈晓明, 罗鹏飞, 邵培坚, 等.肝癌栓塞化疗生存3年以上48例临床分析.中华放射学杂志, 1995, 29(12):876-877.
16.	Lau WY, Lai EC. The current role of radiofrequency ablation in the management of hepatocellular carcinoma:a systematic review. Ann Surg, 2009, 249(1):20-25.
17.	Buscarini L, Rossi S. Technology for radiofrequency thermal ablation of liver tumors. Semin Laparosc Surg, 1997, 4(2):96-101.
18.	Ali MY, Grimm CF, Ritter M, et a1. Activation of dendritic cells by local ablation of hepatocellular carcinoma. J Hepatol, 2005, 43(5):817-822.
19.	郭颖, 乔正荣.射频消融治疗恶性肿瘤现状.中华肿瘤防治杂志, 2006, 13(9):703-706.
20.	梁萍, 董宝玮, 于小玲, 等.超声引导下经皮微波凝固治疗肝癌的临床应用.中华肿瘤杂志, 1997, 19(6):448-450.
21.	刘君, 蒲永东, 范志忠, 等.西米替丁对大肠癌根治术后患者的免疫调节作用及临床意义.中华普通外科杂志, 2002, 17(2):72-74.
22.	Seki S, Habu Y, Kawamura T, et al. The liver as a crucial organ in the first line of host defense:the roles of Kupffer cells, natural killer (NK) cells and NK1.1 Ag⁺ T cells in T helper 1 immune responses. Immunol Rev, 2000, 174:35-46.
23.	Smyth MJ, Thia KY, Street SE, et al. Defferential tumor surveillance by NK and NKT cells. J Exp Med, 2000, 191(4):661-668.

1. Choti MA. Surgical management of hepatocellular carcinoma:resection and ablation. J Vasc Interv Radiol, 2002, 13(9 Pt 2):S197-S203.
2. 汤钊猷.原发性肝癌.第2版.上海:上海科学技术出版社, 1999:182-185.
3. 黄建钊, 刘江伟, 孙倩, 等.射频消融与手术切除治疗肝癌的疗效比较.广东医学杂志, 2012, 33(13):1991-1993.
4. Chan AC, Poon RT, Cheung TT, et al. Survival analysis of re-resection versus radiofrequency ablation for intrahepatic recurrence after hepatectomy for hepatocellular carcinoma. World J Surg, 2012, 36(1):151-156.
5. 陈敏华.微创时代肝癌的射频消融治疗.中华消化外科杂志, 2009, 8(1):4-6.
6. 芶欣, 黄建钊, 孙倩, 等.经皮集束射频消融治疗肝血管瘤的近期疗效观察.广东医学, 2011, 32(7):889-890.
7. Buscarini E, Savoia A, Brambilla G, et al. Radiofrequency thermal ablation of liver tumors. Eur Radiol, 2010, 15(5):884-894.
8. Truk MJ, Guevara-Patiño JA, Rizzuto GA, et al. Concomitant tumor immunity to a poorly immunogenic melanoma is prevented by regulatory T cells. J Exp Med, 2011, 200(6):771-782.
9. Ormandy LA, Hillemann T, Wedemeyer H, et al. Inreased populations of regulatory T cells in peripheral blood of patients with hepatocellular. Cancer Res, 2005, 65(6):2457-2464.
10. Jordan MS, Riley MP, von Boehmer H, et al. Anergy and suppression regulate CD4(+) T cell responses to a self peptide. Eur J Immunol, 2000, 30(1):136-144.
11. Smyth MJ, Thia KY, Street SE, C, et al. Defferential tumor surveillance by NK and NKT cells. J Exp Med, 2000, 191(4):661-668.
12. Liu Y, Poon RT, Hughes J, et al. Chemokine receptions support infiltration of lymphocyte subpopulations in human hepatocelluar carcinoma. Clin Immunol, 2005, 114(2):174-182.
13. Bentdal O, FrØland SS, Bosnes V, et al. Alteration in lymphocyte subsets in blood may predict resectibility in carcinoma of cardia or oesophogus. Cancer Letters, 1996, 100(1-2):133-138.
14. 周永昌, 郭万学.超声医学.第4版.北京:北京科学技术文献出版社, 2003:1622-1624.
15. 陈晓明, 罗鹏飞, 邵培坚, 等.肝癌栓塞化疗生存3年以上48例临床分析.中华放射学杂志, 1995, 29(12):876-877.
16. Lau WY, Lai EC. The current role of radiofrequency ablation in the management of hepatocellular carcinoma:a systematic review. Ann Surg, 2009, 249(1):20-25.
17. Buscarini L, Rossi S. Technology for radiofrequency thermal ablation of liver tumors. Semin Laparosc Surg, 1997, 4(2):96-101.
18. Ali MY, Grimm CF, Ritter M, et a1. Activation of dendritic cells by local ablation of hepatocellular carcinoma. J Hepatol, 2005, 43(5):817-822.
19. 郭颖, 乔正荣.射频消融治疗恶性肿瘤现状.中华肿瘤防治杂志, 2006, 13(9):703-706.
20. 梁萍, 董宝玮, 于小玲, 等.超声引导下经皮微波凝固治疗肝癌的临床应用.中华肿瘤杂志, 1997, 19(6):448-450.
21. 刘君, 蒲永东, 范志忠, 等.西米替丁对大肠癌根治术后患者的免疫调节作用及临床意义.中华普通外科杂志, 2002, 17(2):72-74.
22. Seki S, Habu Y, Kawamura T, et al. The liver as a crucial organ in the first line of host defense:the roles of Kupffer cells, natural killer (NK) cells and NK1.1 Ag⁺ T cells in T helper 1 immune responses. Immunol Rev, 2000, 174:35-46.
23. Smyth MJ, Thia KY, Street SE, et al. Defferential tumor surveillance by NK and NKT cells. J Exp Med, 2000, 191(4):661-668.

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Changes of Peripheral Blood Lymphocyte Subsets of Patients with Primary Hepatocellular Carcinoma Treated with Radiofrequency Ablation

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