Experimental Study of Colonic Microflora Imbalance in Patients with Obstructive Jaundice_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 ZHENGPeng , FENGTao , ZHAOXin , KAOXiao-ming ,  JIWu

Nanjing University School of Medicine/General Surgery of Nanjing General Hospital of Nanjing Military Command, Nanjing 210002, Jiangsu Province, China;

Corresponding author：

JIWu, Email: jiwuvip@hotmail.com

Keywords：

Obstructive jaundice; Intestinal flora; Polymerase chain reaction; Denaturing gradient gel electrophoresis; Principal component analysis

DOI：

10.7507/1007-9424.20150313

Video：

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Objective To explore the variation of the structure of the intestinal flora between healthy people and patients with obstructive jaundice perioperatively. Methods From February 2013 to August 2014, 20 patients with obstructive jaundice and 10 healthy persons (normal control group) in our hospitol were selected as the research object. The first stool specimens of the research object after admission were obtained and the total fecal bacteria DNA were extracted. After polymerase chain reaction amplification, the changes in the structure of bacterial flora were dynamic observed by using denaturing gradient gel electrophoresis (DGGE), and the gel bands were analyzed by using Quantity One software. The similarity and diversity of flora structure, and principal component analysis (PCA) were analyzed. Results Significant differences of colonic microflora were found between patients with obstructive jaundice and healthy people; advantage intestinal flora in obstructive jaundice patients was significant lower than the normal control group. With the extension of time and degree of obstruction aggravated, a descending trend was found in number, abundance, and diversity of the intestinal microflora (P < 0.05). Conclusion There is significant differences in the structure of colon bacteria in patients with obstructive jaundice and healthy persons.

Citation： ZHENGPeng, FENGTao, ZHAOXin, KAOXiao-ming, JIWu. Experimental Study of Colonic Microflora Imbalance in Patients with Obstructive Jaundice. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(10): 1209-1213. doi: 10.7507/1007-9424.20150313 Copy

1.	Lorenzo-Zúñiga V, Bartolí R, Planas R, et al. Oral bile acids reduce bacterial overgrowth, bacterial translocation, and endotoxemia in cirrhotic rats. Hepatology, 2003, 37(3):551-557.
2.	徐俊超, 孙备, 李军, 等.梗阻性黄疸术后并发应激性黏膜病变4例报告.中国实用外科杂志, 2011, 31(10):979-980.
3.	Rauch M, Lynch SV. The potential for probiotic manipulation of the gastrointestinal microbiome. Curr Opin Biotechnol, 2012, 23(2):192-201.
4.	周中凯, 杨艳, 郑排云, 等.肠道微生物蛋白质的发酵与肠道健康的关系.食品科学, 2014, 35(1):303-309.
5.	Gothe F, Beigel F, Rust C, et al. Bile acid malabsorption assessed by 7 alpha-hydroxy-4-cholesten-3-one in pediatric inflammatory bowel disease:Correlation to clinical and laboratory findings. J Crohns Colitis, 2014, 8(9):1072-1078.
6.	Arslan N. Obesity, fatty liver disease and intestinal microbiota. World J Gastroenterol, 2014, 20(44):16452-16463.
7.	张雪群, 马燕燕.肠-肝轴研究进展.中国科技论文在线. 2013. http://www.paper.edu.cn.
8.	Trauner M, Fickert P, Tilg H. Bile acids as modulators of gut microbiota linking dietary habits and inflammatory bowel disease:A potentially dangerous liaison. Gastroenterology, 2013, 144(4):844-846.
9.	Liu HX, Keane R, Sheng L, et al. The immunomodulatory role of bile acids. J Hepatol. 2015, [Epub ahead of print].
10.	Ridlon JM, Alves JM, Hylemon PB, et al. Cirrhosis, bile acids and gut microbiota:Unraveling a complex relationship. Gut Microbes, 2013, 4(5):382-387.
11.	Ley RE, Bäckhed F, Turnbaugh P, et al. Obesity alters gut microbial ecology. Proc Natl Acad Sci U S A, 2005, 102(31):11070-11075.
12.	Mai V, Colbert LH, Berrigan D, et al. Calorie restriction and diet composition modulate spontaneous intestinal tumorigenesis in Apc (Min) mice through different mechanisms. Cancer Res, 2003, 63(8):1752-1755.
13.	华静, 李继强.肝硬化患者肠道菌群的研究.中华肝脏病杂志, 1998, 6(2):79-81.
14.	Othman M, Agüero R, Lin HC. Alterations in intestinal microbial flora and human disease. Curr Opin Gastroenterol, 2008, 24(1):11-16.
15.	Sourianarayanane A, Garg G, Smith TH, et al. Risk factors of nonalcoholic fatty liver disease in patients with inflammatory bowel disease. J Crohns Colitis, 2013, 7(8):e279-e285.
16.	Arrieta MC, Finlay BB. The commensal microbiota drives immune homeostasis. Front Immunol, 2012, 3:33.
17.	Chassaing B, Etienne-Mesmin L, Gewirtz AT. Microbiota-liver axis in hepatic disease. Hepatology, 2014, 59(1):328-339.
18.	Scott KP, Duncan SH, Louis P, et al. Nutritional influences on the gut microbiota and the consequences for gastrointestinal health. Biochem Soc Trans, 2011, 39(4):1073-1078.
19.	Rumstadt B, Schwab M, Korth P, et al. Hemorrhage after pancreatoduodenectomy. Ann Surg, 1998, 227(2):236-241.
20.	Srivastava S, Sikora SS, Kumar A, et al. Outcome following pancreaticoduodenectomy in patients undergoing preoperative biliary drainage. Dig Surg, 2001, 18(5):381-387.
21.	Cakir T, Cingi A, Yeğen C. Coagulation dynamics and platelet functions in obstructive jaundiced patients. J Gastroenterol Hepatol, 2009, 24(5):748-751.
22.	Xiong JJ, Nunes QM, Huang W, et al. Preoperative biliary drainage in patients with hilar cholangiocarcinoma undergoing major hepatectomy. World J Gastroenterol, 2013, 19(46):8731-8739.
23.	Yoshida Y, Ajiki T, Ueno K, et al. Preoperative bile replacement improves immune function for jaundiced patients treated with external biliary drainage. J Gastrointest Surg, 2014, 18(12):2095-2104.
24.	Hsieh CS, Huang LT, Huang CC, et al. Bacteria ascend to liver from the bilioenteric conduit after choledochojejunostomy in the cholestatic rat. Pediatr Surg Int, 2003, 19(11):699-702.
25.	Liu HX, Keane R, Sheng L, et al. Implications of microbiota and bile acid in liver injury and regeneration. J Hepatol, 2015, [Epub ahead of print].

1. Lorenzo-Zúñiga V, Bartolí R, Planas R, et al. Oral bile acids reduce bacterial overgrowth, bacterial translocation, and endotoxemia in cirrhotic rats. Hepatology, 2003, 37(3):551-557.
2. 徐俊超, 孙备, 李军, 等.梗阻性黄疸术后并发应激性黏膜病变4例报告.中国实用外科杂志, 2011, 31(10):979-980.
3. Rauch M, Lynch SV. The potential for probiotic manipulation of the gastrointestinal microbiome. Curr Opin Biotechnol, 2012, 23(2):192-201.
4. 周中凯, 杨艳, 郑排云, 等.肠道微生物蛋白质的发酵与肠道健康的关系.食品科学, 2014, 35(1):303-309.
5. Gothe F, Beigel F, Rust C, et al. Bile acid malabsorption assessed by 7 alpha-hydroxy-4-cholesten-3-one in pediatric inflammatory bowel disease:Correlation to clinical and laboratory findings. J Crohns Colitis, 2014, 8(9):1072-1078.
6. Arslan N. Obesity, fatty liver disease and intestinal microbiota. World J Gastroenterol, 2014, 20(44):16452-16463.
7. 张雪群, 马燕燕.肠-肝轴研究进展.中国科技论文在线. 2013. http://www.paper.edu.cn.
8. Trauner M, Fickert P, Tilg H. Bile acids as modulators of gut microbiota linking dietary habits and inflammatory bowel disease:A potentially dangerous liaison. Gastroenterology, 2013, 144(4):844-846.
9. Liu HX, Keane R, Sheng L, et al. The immunomodulatory role of bile acids. J Hepatol. 2015, [Epub ahead of print].
10. Ridlon JM, Alves JM, Hylemon PB, et al. Cirrhosis, bile acids and gut microbiota:Unraveling a complex relationship. Gut Microbes, 2013, 4(5):382-387.
11. Ley RE, Bäckhed F, Turnbaugh P, et al. Obesity alters gut microbial ecology. Proc Natl Acad Sci U S A, 2005, 102(31):11070-11075.
12. Mai V, Colbert LH, Berrigan D, et al. Calorie restriction and diet composition modulate spontaneous intestinal tumorigenesis in Apc (Min) mice through different mechanisms. Cancer Res, 2003, 63(8):1752-1755.
13. 华静, 李继强.肝硬化患者肠道菌群的研究.中华肝脏病杂志, 1998, 6(2):79-81.
14. Othman M, Agüero R, Lin HC. Alterations in intestinal microbial flora and human disease. Curr Opin Gastroenterol, 2008, 24(1):11-16.
15. Sourianarayanane A, Garg G, Smith TH, et al. Risk factors of nonalcoholic fatty liver disease in patients with inflammatory bowel disease. J Crohns Colitis, 2013, 7(8):e279-e285.
16. Arrieta MC, Finlay BB. The commensal microbiota drives immune homeostasis. Front Immunol, 2012, 3:33.
17. Chassaing B, Etienne-Mesmin L, Gewirtz AT. Microbiota-liver axis in hepatic disease. Hepatology, 2014, 59(1):328-339.
18. Scott KP, Duncan SH, Louis P, et al. Nutritional influences on the gut microbiota and the consequences for gastrointestinal health. Biochem Soc Trans, 2011, 39(4):1073-1078.
19. Rumstadt B, Schwab M, Korth P, et al. Hemorrhage after pancreatoduodenectomy. Ann Surg, 1998, 227(2):236-241.
20. Srivastava S, Sikora SS, Kumar A, et al. Outcome following pancreaticoduodenectomy in patients undergoing preoperative biliary drainage. Dig Surg, 2001, 18(5):381-387.
21. Cakir T, Cingi A, Yeğen C. Coagulation dynamics and platelet functions in obstructive jaundiced patients. J Gastroenterol Hepatol, 2009, 24(5):748-751.
22. Xiong JJ, Nunes QM, Huang W, et al. Preoperative biliary drainage in patients with hilar cholangiocarcinoma undergoing major hepatectomy. World J Gastroenterol, 2013, 19(46):8731-8739.
23. Yoshida Y, Ajiki T, Ueno K, et al. Preoperative bile replacement improves immune function for jaundiced patients treated with external biliary drainage. J Gastrointest Surg, 2014, 18(12):2095-2104.
24. Hsieh CS, Huang LT, Huang CC, et al. Bacteria ascend to liver from the bilioenteric conduit after choledochojejunostomy in the cholestatic rat. Pediatr Surg Int, 2003, 19(11):699-702.
25. Liu HX, Keane R, Sheng L, et al. Implications of microbiota and bile acid in liver injury and regeneration. J Hepatol, 2015, [Epub ahead of print].

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Experimental Study of Colonic Microflora Imbalance in Patients with Obstructive Jaundice

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