Expression of c-Met in Colorectal Carcinoma Cells and Effect of Hepatocyte Growth Factor on Proliferation and Invasion of Colon Carcinoma Cells SW480_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 YAOJian-feng ¹ ,  SUNXue-jun ² , YANLi-kun ¹ , HESai ² , LIXiao-jun ¹ , TONGCong ¹ , WANGXiao-qiang ¹

1. Department of General Surgery, Shaanxi Provincial People's Hospital, Xi'an 710068, Shaanxi Province, China;
2. Department of General Surgery, The First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China;

Corresponding author：

SUNXue-jun, Email: sunxy@mail.xjtu.edu.cn

Keywords：

c-Met protein; Colorectal neoplasm; Hepatocyte growth factor; Pro1iferation; Invasion

DOI：

10.7507/1007-9424.20150344

Video：

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Objective To study the expression of c-Met in colorectal carcinoma cells and the effect of hepatocyte growth factor (HGF) on proliferation and invasion of colon carcinoma cells SW480. Methods Real-time PCR and Western blot methods were respectively used to detect the expressions of c-Met mRNA and protein in the different colorectal carcinoma cells in order to screen the high c-Met expression cells. The SW480 cells were incubated with different concentrations (0, 20, 40, and 70 ng/mL) HGF. MTT assay and Transwell test were used to evaluate the effects of proliferation and invasion in the SW480 cells. Results ①The c-Met was expressed in each colorectal carcinomar cells, especially highly expressed in the colon carcinoma cells SW480 in vitro.②MTT assay showed that the HGF could promote the proliferation of SW480 cells in a dose-dependent manner with some extent.③Transwell test showed that the HGF could increase the invasion of SW480 cells. Conclusion The c-Met is highly expressed in colorectal carcinoma cells and HGF could promote proliferation and increase invasion of colorectal carcinoma cells in vitro.

Citation： YAOJian-feng, SUNXue-jun, YANLi-kun, HESai, LIXiao-jun, TONGCong, WANGXiao-qiang. Expression of c-Met in Colorectal Carcinoma Cells and Effect of Hepatocyte Growth Factor on Proliferation and Invasion of Colon Carcinoma Cells SW480. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(11): 1314-1318. doi: 10.7507/1007-9424.20150344 Copy

1.	Zeng Q, Chen S, You Z, et al. Hepatocyte growth factor inhibits anoikis in head and neck squamous cell carcinoma cells by activation of ERK and Akt signaling independent of NFkappa B. J Biol Chem, 2002, 277(28):25203-25208.
2.	Jung KH, Park BH, Hong SS. Progress in cancer therapy targeting c-Met signaling pathway. Arch Pharm Res, 2012, 35(4):595-604.
3.	Shojaei F, Simmons BH, Lee JH, et al. HGF/c-Met pathway is one of the mediators of sunitinib-induced tumor cell type-dependent metastasis. Cancer Lett, 2012, 320(1):48-55.
4.	姚建锋, 孙学军, 闫立昆, 等. c-Met在有、无肝转移结直肠癌组织中的表达差异.中国普通外科杂志, 2014, 23(4):468-472.
5.	Madonna R, Cevik C, Nasser M, et al. Hepatocyte growth factor:molecular biomarker and player in cardioprotection and cardiovascular regeneration. Thromb Haemost, 2012, 107(4):656-661.
6.	Stella GM, Benvenuti S, Gramaglia D, et al. Met mutations in cancers of unknown primary origin (CUPs). Hum Mutat, 2011, 32(1):44-50.
7.	Yap TA, Sandhu SK, Alam SM, et al. HGF/c-MET targeted therapeutics:novel strategies for cancer medicine. Curr Drug Targets, 2011, 12(14):2045-2058.
8.	Sierra JR, Tsao MS. c-MET as a potential therapeutic target and biomarker in cancer. Ther Adv Med Oncol, 2011, 3(1 Suppl):S21-S35.
9.	Akasaka Y, Ono I, Yamashita T, et al. Basic fibroblast growth factor promotes apoptosis and suppresses granulation tissue formation in acute incisional wounds. J Pathol, 2004, 203(2):710-720.
10.	Cotrim P, Martelli-Junior H, Graner E, et al. Cyclosporin a induces proliferation in human gingival fibroblasts via induction of transforming growth factor-beta1. J Periodontol, 2003, 74(11):1625-1633.
11.	Stanley G, Harvey K, Slivova V, et al. Ganoderma lucidum suppresses angiogenesis through the inhibition of secretion of VEGF and TGF-beta1 from prostate cancer cells. Biochem Biophys Res Commun, 2005, 330(1):46-52.
12.	Webb CP, Taylor GA, Jeffers M, et al. Evidence for a role of Met-HGF/SF during Ras-mediated tumorigenesis/metastasis. Oncogene, 1998, 17(16):2019-2025.
13.	王立生, 刘福陆, 贺福初, 等.纯化肝细胞生长因子对肝癌细胞的增殖刺激作用.高技术通讯, 1995, 5(8):48-50.
14.	Liu T, Li Q, Sun Q, et al. Met inhibitor PHA-665752 suppresses the hepatocyte growth factor-induced cell proliferation and radioresistance in nasopharyngeal carcinoma cells. Biochem Biophys Res Commun, 2014, 449(1):49-54.
15.	石玉生, 谭燕, 伍锡栋, 等. c-Met siRNA对U87-EGFRvⅢ胶质瘤细胞凋亡和增殖的影响.实用医学杂志, 2013, 29(6):854-856.
16.	Kermorgant S, Aparicio T, Dessirier V, et al. Hepatocyte growth factor induces colonic cancer cell invasiveness via enhanced motility and protease overproduction. Evidence for PI3 kinase and PKC involvement. Carcinogenesis, 2001, 22(7):1035-1042.
17.	Shimizu S, Yamada N, Sawada T, et al. In vivo and in vitro interactions between human colon carcinoma cells and hepatic stellate cells. Jpn J Cancer Res, 2000, 91(12):1285-1295.
18.	Dinev D, Jordan BW, Neufeld B, et al. Extracellular signal regulated kinase 5(ERK5) is required for the differentiation of muscle cells. EMBO Rep, 2001, 2(9):829-834.
19.	Hiscox S, Jiang WG. Association of the HGF/SF receptor, c-met, with the cell-surface adhesion molecule, E-cadherin, and catenins in human tumor cells. Biochem Biophys Res Commun, 1999, 261(2):406-411.
20.	Ramanujum R, Lin YL, Liu JK, et al. Regulatory expression of MMP-8/MMP-9 and inhibition of proliferation, migration and invasion in human lung cancer A549 cells in the presence of HGF variants. Kaohsiung J Med Sci, 2013, 29(10):530-539.

1. Zeng Q, Chen S, You Z, et al. Hepatocyte growth factor inhibits anoikis in head and neck squamous cell carcinoma cells by activation of ERK and Akt signaling independent of NFkappa B. J Biol Chem, 2002, 277(28):25203-25208.
2. Jung KH, Park BH, Hong SS. Progress in cancer therapy targeting c-Met signaling pathway. Arch Pharm Res, 2012, 35(4):595-604.
3. Shojaei F, Simmons BH, Lee JH, et al. HGF/c-Met pathway is one of the mediators of sunitinib-induced tumor cell type-dependent metastasis. Cancer Lett, 2012, 320(1):48-55.
4. 姚建锋, 孙学军, 闫立昆, 等. c-Met在有、无肝转移结直肠癌组织中的表达差异.中国普通外科杂志, 2014, 23(4):468-472.
5. Madonna R, Cevik C, Nasser M, et al. Hepatocyte growth factor:molecular biomarker and player in cardioprotection and cardiovascular regeneration. Thromb Haemost, 2012, 107(4):656-661.
6. Stella GM, Benvenuti S, Gramaglia D, et al. Met mutations in cancers of unknown primary origin (CUPs). Hum Mutat, 2011, 32(1):44-50.
7. Yap TA, Sandhu SK, Alam SM, et al. HGF/c-MET targeted therapeutics:novel strategies for cancer medicine. Curr Drug Targets, 2011, 12(14):2045-2058.
8. Sierra JR, Tsao MS. c-MET as a potential therapeutic target and biomarker in cancer. Ther Adv Med Oncol, 2011, 3(1 Suppl):S21-S35.
9. Akasaka Y, Ono I, Yamashita T, et al. Basic fibroblast growth factor promotes apoptosis and suppresses granulation tissue formation in acute incisional wounds. J Pathol, 2004, 203(2):710-720.
10. Cotrim P, Martelli-Junior H, Graner E, et al. Cyclosporin a induces proliferation in human gingival fibroblasts via induction of transforming growth factor-beta1. J Periodontol, 2003, 74(11):1625-1633.
11. Stanley G, Harvey K, Slivova V, et al. Ganoderma lucidum suppresses angiogenesis through the inhibition of secretion of VEGF and TGF-beta1 from prostate cancer cells. Biochem Biophys Res Commun, 2005, 330(1):46-52.
12. Webb CP, Taylor GA, Jeffers M, et al. Evidence for a role of Met-HGF/SF during Ras-mediated tumorigenesis/metastasis. Oncogene, 1998, 17(16):2019-2025.
13. 王立生, 刘福陆, 贺福初, 等.纯化肝细胞生长因子对肝癌细胞的增殖刺激作用.高技术通讯, 1995, 5(8):48-50.
14. Liu T, Li Q, Sun Q, et al. Met inhibitor PHA-665752 suppresses the hepatocyte growth factor-induced cell proliferation and radioresistance in nasopharyngeal carcinoma cells. Biochem Biophys Res Commun, 2014, 449(1):49-54.
15. 石玉生, 谭燕, 伍锡栋, 等. c-Met siRNA对U87-EGFRvⅢ胶质瘤细胞凋亡和增殖的影响.实用医学杂志, 2013, 29(6):854-856.
16. Kermorgant S, Aparicio T, Dessirier V, et al. Hepatocyte growth factor induces colonic cancer cell invasiveness via enhanced motility and protease overproduction. Evidence for PI3 kinase and PKC involvement. Carcinogenesis, 2001, 22(7):1035-1042.
17. Shimizu S, Yamada N, Sawada T, et al. In vivo and in vitro interactions between human colon carcinoma cells and hepatic stellate cells. Jpn J Cancer Res, 2000, 91(12):1285-1295.
18. Dinev D, Jordan BW, Neufeld B, et al. Extracellular signal regulated kinase 5(ERK5) is required for the differentiation of muscle cells. EMBO Rep, 2001, 2(9):829-834.
19. Hiscox S, Jiang WG. Association of the HGF/SF receptor, c-met, with the cell-surface adhesion molecule, E-cadherin, and catenins in human tumor cells. Biochem Biophys Res Commun, 1999, 261(2):406-411.
20. Ramanujum R, Lin YL, Liu JK, et al. Regulatory expression of MMP-8/MMP-9 and inhibition of proliferation, migration and invasion in human lung cancer A549 cells in the presence of HGF variants. Kaohsiung J Med Sci, 2013, 29(10):530-539.

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Expression of c-Met in Colorectal Carcinoma Cells and Effect of Hepatocyte Growth Factor on Proliferation and Invasion of Colon Carcinoma Cells SW480

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