Effects of Hypertonic Saline on Levels of Nitric Oxide and Endothelin-1 in Hepatic Ischemia-Reperfusion Injury Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 HUANGTao ¹ , ERQi-dong ¹ , DENGFeng ¹ , BIXu-dong ²

1. Department of General Surgery, Xiqing Hospital of Tianjin, Tianjin 300380, China;
2. Department of General Surgery, The First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, Liaoning Province, China;

Corresponding author：

HUANGTao, Email: htwww2008@126.com

Keywords：

Rat; Liver; Ischemia reperfusion injury; Hypertonic saline; Nitric oxide; Endothelin-1

DOI：

10.7507/1007-9424.20150346

Video：

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Objective To explore the effect of hypertonic saline (HTS) pretreatment on levels of nitric oxide (NO) and endothelin-1(ET-1) and their correlation in hepatic ischemia reperfusion (HIR) injury in rats. Methods The HIR injury models were made by using Pringle, s maneuver in 45 healthy adult male Sprague-Dawley rats, which were randomly divided into three groups (n=15):sham operation (SO) group, HIR group, and HTS group. The animals were killed at 1, 6, and 24 h after reperfusion. The levels of serum NO and ET-1 were measured respectively, the correlation between NO level and ET-1 level at 6 h after reperfusion was analyzed. Results At the time points of 1 h, 6 h, and 24 h after reperfusion, the serum NO levels in the HTS group and HIR group were all significantly lower than those in the SO group (P < 0.01), but the serum ET-1 levels were all significantly higher than those in the so group (P < 0.01). The serum NO levels at the time points of 1 h, 6 h, and 24 h in the HTS group were significantly higher than those at the same time in the HIR group (P < 0.01), but the serum ET-1 levels in the HTS group were significantly lower than those in the HIR group (P < 0.01). At all the time points, every detected goal had more marked level at the time point of 6 h after reperfusion. The NO level was negatively correlated with the ET-1 level. Conclusions HTS could change levels of serum NO and ET-1 after HIR injury, and which has a negative correlation. Its mechanism might probably stimulate serum NO level and reduce the ET-1 level through some way so as to enable both dynamic balance to the benign development direction and achieve a protective effect.

Citation： HUANGTao, ERQi-dong, DENGFeng, BIXu-dong. Effects of Hypertonic Saline on Levels of Nitric Oxide and Endothelin-1 in Hepatic Ischemia-Reperfusion Injury Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(11): 1323-1326. doi: 10.7507/1007-9424.20150346 Copy

1.	Yada-Langui MM, Anjos-Valotta EA, Sannomiya P, et al. Resuscitation affects microcirculatory polymorphonuclear leukocyte behavior after hemorrhagic shock:role of hypertonic saline and pentoxifylline. Exp Biol Med (Maywood), 2004, 229(7):684-693.
2.	黄涛, 毕旭东.高渗盐水预处理对肝脏缺血再灌注大鼠血清肿瘤坏死因子-α和IL-10水平的影响.中国全科医学, 2008, 11(18):1649-1652.
3.	闻静, 张敬莹, 仓彦, 等.高渗盐水预处理在大鼠缺血再灌注心肌损伤中的保护作用.同济大学学报:医学版, 2015, 36(2):20-23.
4.	赵晶, 毕旭东, 穆长征, 等.高渗盐水对大鼠肝脏缺血再灌注损伤的保护作用及其机制.中国老年学杂志, 2013, 33(5):1096-1100.
5.	Scrracion-Lnglott F, Habib N, Mathie RT. Hepatic ischemia reperfusion injury. Am J Surg, 2001, 181(2):160-166.
6.	Fondevila C, Busuttil RW, Kupiec-Weglinski JW. Hepatic ischemia/reperfusion injury-a fresh look. Exp Mol Pathol, 2003, 74(2):86-93.
7.	贾长库, 陈有科, 韩霖, 等.交替半肝入肝血流阻断解剖性肝中叶切除.中国普通外科杂志, 2015, 24(1):121-126.
8.	Kohr MJ, Sun J, Aponte A, et al. Simultaneous measurement of protein oxidation and S-nitrosylation during preconditioning and ischemia/reperfusion injury with resin-assisted capture. Circ Res, 2011, 108(4):418-426.
9.	戴勇, 陶立德, 薛同敏, 等.缺血预处理对大鼠肝脏缺血再灌注损伤后细胞凋亡的影响.中国现代普通外科进展, 2015, 18(3):169-172.
10.	张召辉, 王为忠, 李玺, 等. IPC、IPO和IPC-IPO对胰腺冷缺血再灌注损伤保护作用的对比研究.中国普外基础与临床杂志, 2011, 18(8):844-848.
11.	Costa FL, Yamaki VN, Gonçalves TB, et al. Combined remote ischemic perconditioning and local postconditioning on liver ischemia-reperfusion injury. J Surg Res, 2014, 192(1):98-102.
12.	李俊, 王健, 李鹏飞, 等.间断低氧预适应对大鼠肝切除缺血再灌注损伤的保护作用.中国普外基础与临床杂志, 2014, 21(10):1284-1286.
13.	Kislin MS, Tiul, kova EI, Samoĭlov MO. Dynamics of lipid peroxidation of membranes in cells and mitochondrial fraction of neocortex in non-and preconditioned rats after severe hypobaric hypoxia. Zh Evol Biokhim Fiziol, 2011, 47(2):157-164.
14.	朱耿隆, 蔡潮农, 贾英斌, 等.法舒地尔对阻塞性黄疸大鼠肝缺血再灌注损伤的影响.中华肝胆外科杂志, 2015, 21(1):44-48.
15.	Zhang N, Cheng GY, Liu XZ, et al. Expression of Bcl-2 and NF-κB in brain tissue after acute renal ischemia-reperfusion in rats. Asian Pac J Trop Med, 2014, 7(5):386-389.
16.	张森森, 朱振新, 吴洪坤, 等.高渗盐水复苏缺血小肠对小肠免疫屏障和系统炎性反应综合征的影响.中国实验诊断学, 2014, 18(2):187-191, 349.
17.	朱高峰, 邓医宇, 江稳强, 等.高渗盐水抑制肿瘤坏死因子-α表达与减轻脑水肿的相关性.实用医学杂志, 2012, 28(10):1618-1621.
18.	Oddo MS. Effect of mannitol and hypertonic saline on cerebral oxygenation in patients with severe traumatic brain injury and refractory intracranial hypertension. J Neulol Neurosurg Psychiatry, 2009, 80(8):916-920.
19.	李汉臣, 秦兆寅, 纪宗正, 等.高渗盐水治疗对失血性休克鼠网状内皮细胞功能和易感染性的影响.中国普外基础与临床杂志, 2004, 11(1):49-51.
20.	Inagami T, Naruse M, Hoover R. Endothelium as an endocrine organ. Annu Rev Physiol, 1995, 57:171-189.
21.	Carter EP, Sato K, Morio Y, et al. Inhibition of K(Ca) channels restores blunted hypoxic pulmonary vasoconstriction in rats with cirrhosis. Am J Physiol Lung Cell Mol Physiol, 2000, 279(5):L903-L910.
22.	李旭明.乌司他丁对肝缺血再灌注损伤患者血浆一氧化氮/内皮素-1和炎症细胞因子的调控作用.实用医学杂志, 2009, 25(8):1293-1295.
23.	袁晟光, 董家鸿.一氧化氮在肝脏病变中的作用.消化外科, 2003, 2(3):209-213.
24.	Nishida J, Mccuskey RS, Mcdonnell D, et al. Protective role of NO in hepatic microcirculatory dysfunction during endotoxemia. Am J Physiol, 1994, 267(6 Pt 1):G1135-G1141.
25.	Urakami A, Todo S, Zhu Y, et al. Attenuation of ischemic liver injury by monoclonal anti-endothelin antibody, AwETN40. J Am Coll Surg, 1997, 185(4):358-364.
26.	郎洁, 张培建.板蓝根多糖对大鼠自体肝移植后一氧化氮和内皮素-1的影响.军事医学, 2014, 38(10):814-818.

1. Yada-Langui MM, Anjos-Valotta EA, Sannomiya P, et al. Resuscitation affects microcirculatory polymorphonuclear leukocyte behavior after hemorrhagic shock:role of hypertonic saline and pentoxifylline. Exp Biol Med (Maywood), 2004, 229(7):684-693.
2. 黄涛, 毕旭东.高渗盐水预处理对肝脏缺血再灌注大鼠血清肿瘤坏死因子-α和IL-10水平的影响.中国全科医学, 2008, 11(18):1649-1652.
3. 闻静, 张敬莹, 仓彦, 等.高渗盐水预处理在大鼠缺血再灌注心肌损伤中的保护作用.同济大学学报:医学版, 2015, 36(2):20-23.
4. 赵晶, 毕旭东, 穆长征, 等.高渗盐水对大鼠肝脏缺血再灌注损伤的保护作用及其机制.中国老年学杂志, 2013, 33(5):1096-1100.
5. Scrracion-Lnglott F, Habib N, Mathie RT. Hepatic ischemia reperfusion injury. Am J Surg, 2001, 181(2):160-166.
6. Fondevila C, Busuttil RW, Kupiec-Weglinski JW. Hepatic ischemia/reperfusion injury-a fresh look. Exp Mol Pathol, 2003, 74(2):86-93.
7. 贾长库, 陈有科, 韩霖, 等.交替半肝入肝血流阻断解剖性肝中叶切除.中国普通外科杂志, 2015, 24(1):121-126.
8. Kohr MJ, Sun J, Aponte A, et al. Simultaneous measurement of protein oxidation and S-nitrosylation during preconditioning and ischemia/reperfusion injury with resin-assisted capture. Circ Res, 2011, 108(4):418-426.
9. 戴勇, 陶立德, 薛同敏, 等.缺血预处理对大鼠肝脏缺血再灌注损伤后细胞凋亡的影响.中国现代普通外科进展, 2015, 18(3):169-172.
10. 张召辉, 王为忠, 李玺, 等. IPC、IPO和IPC-IPO对胰腺冷缺血再灌注损伤保护作用的对比研究.中国普外基础与临床杂志, 2011, 18(8):844-848.
11. Costa FL, Yamaki VN, Gonçalves TB, et al. Combined remote ischemic perconditioning and local postconditioning on liver ischemia-reperfusion injury. J Surg Res, 2014, 192(1):98-102.
12. 李俊, 王健, 李鹏飞, 等.间断低氧预适应对大鼠肝切除缺血再灌注损伤的保护作用.中国普外基础与临床杂志, 2014, 21(10):1284-1286.
13. Kislin MS, Tiul, kova EI, Samoĭlov MO. Dynamics of lipid peroxidation of membranes in cells and mitochondrial fraction of neocortex in non-and preconditioned rats after severe hypobaric hypoxia. Zh Evol Biokhim Fiziol, 2011, 47(2):157-164.
14. 朱耿隆, 蔡潮农, 贾英斌, 等.法舒地尔对阻塞性黄疸大鼠肝缺血再灌注损伤的影响.中华肝胆外科杂志, 2015, 21(1):44-48.
15. Zhang N, Cheng GY, Liu XZ, et al. Expression of Bcl-2 and NF-κB in brain tissue after acute renal ischemia-reperfusion in rats. Asian Pac J Trop Med, 2014, 7(5):386-389.
16. 张森森, 朱振新, 吴洪坤, 等.高渗盐水复苏缺血小肠对小肠免疫屏障和系统炎性反应综合征的影响.中国实验诊断学, 2014, 18(2):187-191, 349.
17. 朱高峰, 邓医宇, 江稳强, 等.高渗盐水抑制肿瘤坏死因子-α表达与减轻脑水肿的相关性.实用医学杂志, 2012, 28(10):1618-1621.
18. Oddo MS. Effect of mannitol and hypertonic saline on cerebral oxygenation in patients with severe traumatic brain injury and refractory intracranial hypertension. J Neulol Neurosurg Psychiatry, 2009, 80(8):916-920.
19. 李汉臣, 秦兆寅, 纪宗正, 等.高渗盐水治疗对失血性休克鼠网状内皮细胞功能和易感染性的影响.中国普外基础与临床杂志, 2004, 11(1):49-51.
20. Inagami T, Naruse M, Hoover R. Endothelium as an endocrine organ. Annu Rev Physiol, 1995, 57:171-189.
21. Carter EP, Sato K, Morio Y, et al. Inhibition of K(Ca) channels restores blunted hypoxic pulmonary vasoconstriction in rats with cirrhosis. Am J Physiol Lung Cell Mol Physiol, 2000, 279(5):L903-L910.
22. 李旭明.乌司他丁对肝缺血再灌注损伤患者血浆一氧化氮/内皮素-1和炎症细胞因子的调控作用.实用医学杂志, 2009, 25(8):1293-1295.
23. 袁晟光, 董家鸿.一氧化氮在肝脏病变中的作用.消化外科, 2003, 2(3):209-213.
24. Nishida J, Mccuskey RS, Mcdonnell D, et al. Protective role of NO in hepatic microcirculatory dysfunction during endotoxemia. Am J Physiol, 1994, 267(6 Pt 1):G1135-G1141.
25. Urakami A, Todo S, Zhu Y, et al. Attenuation of ischemic liver injury by monoclonal anti-endothelin antibody, AwETN40. J Am Coll Surg, 1997, 185(4):358-364.
26. 郎洁, 张培建.板蓝根多糖对大鼠自体肝移植后一氧化氮和内皮素-1的影响.军事医学, 2014, 38(10):814-818.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Hypertonic Saline on Levels of Nitric Oxide and Endothelin-1 in Hepatic Ischemia-Reperfusion Injury Rats

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