Influence of FoxM1 Expression on Ras and CDK1 Expressions in Thyroid Papillary Carcinoma TPC-1 Cells_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 YUANHao ¹ ,  MINZhi-jun ¹ , CHENJin-hong ² , JIANGXiao-fei ¹ , XUWei-yan ¹ , FENGMao-hua ¹

1. Department of General Surgery, Pudong Hospital of Shanghai, Shanghai 201300, China;
2. Department of General Surgery, Affiliated Huashan Hospital, Fudan University, Shanghai 200040, China;

Corresponding author：

MINZhi-jun, Email: minzhijun0518@126.com

Keywords：

Thyroid papillary carcinoma; FoxM1; MAPK; Signaling pathway; Ras; CDK

DOI：

10.7507/1007-9424.20160046

Video：

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Objective To detect FoxM1 expression in thyroid papillary carcinoma TPC-1 cells,and explore influence of FoxM1 expression on important genes (RAS gene and CDK1 gene) of mitogen activated protein kinase (MAPK) signal pathway. Methods The hFoxM1-RNA interference was used to deal with the thyroid papillary carcinoma TPC-1 cells (experiment group),another untreated TPC-1 cell was as control group.Then the real-time quantitative PCR was used to detect the FoxM1,Ras,and CDK1 gene expressions in all the TPC-1 cells. Result Compared with the control group,the FoxM1 gene expression was significantly decreased (0.452 9 versus 1.005 0,t=24.692 9,P<0.01),the Ras gene expression was significantly elevated (1.319 0 versus 1.001 2,t=14.218 5,P<0.01),and the CDK1 gene expression was significantly decreased (0.767 5 versus 1.008 1，t=10.763 4,P<0.01) in the experiment group. Conclusion FoxM1 gene expression level in thyroid papillary carcinoma TPC-1 cells could influence Ras and CDK1 expression,which suggests that its role in thyroid papillary carcinoma might be associated with MAPK signal pathway.

Citation： YUANHao, MINZhi-jun, CHENJin-hong, JIANGXiao-fei, XUWei-yan, FENGMao-hua. Influence of FoxM1 Expression on Ras and CDK1 Expressions in Thyroid Papillary Carcinoma TPC-1 Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(2): 172-176. doi: 10.7507/1007-9424.20160046 Copy

1.	Occhi G, Barollo S, Regazzo D, et al. A constitutive active MAPK/ERK pathway due to BRAF^V600E positively regulates AHR pathway in PTC. Oncotarget, 2015, 6(31):32104-32114.
2.	Davies L, Welch HG. Increasing incidence of thyroid cancer in the United States, 1973-2002. JAMA, 2006, 295(18):2164-2167.
3.	Ramakrishna S, Kim IM, Petrovic V, et al. Myocardium defects and ventricular hypoplasia in mice homozygous null for the Forkhead Box M1 transcription factor. Dev Dyn, 2007, 236(4):1000-1013.
4.	Brezillon N, Lambert-Blot M, Morosan S, et al. Transplanted hepato-cytes over-expressing FoxM1B efficiently repopulate chronically injured mouse liver independent of donor age. Mol Ther, 2007, 15(9):1710-1715.
5.	Korver W, Roose J, Heinen K, et al. The human Trident/HFH-11/ FKHL16 gene:structure, localization, and promoter characterization. Genomics, 1997, 46(3):435-442.
6.	Yau C, Meyer L, Benz S, et al. FOXM1 cistrome predicts breast cancer metastatic outcome better than FOXM1 expression levels or tumor proliferation index. Breast Cancer Res Treat, 2015, 154(1):23-32.
7.	王一茹, 姚斌伟, 张艳, 等. FoxM1 shRNA慢病毒载体构建及感染人前列腺癌细胞的稳定细胞株筛选. 南方医科大学学报, 2015, 35(9):1227-1233.
8.	Zhang HG, Xu XW, Shi XP, et al. Overexpression of forkhead box protein M1 (FOXM1) plays a critical role in colorectal cancer. Clin Transl Oncol, 2015 Sep 14.
9.	袁浩, 陈进宏, 闵志均, 等. FoxM1基因表达与甲状腺乳头状癌TPC-1细胞活性及侵袭性关系的研究. 中华内分泌外科杂志, 2014, 7(4):265-268.
10.	Guan H, Ji M, Bao R, et al. Association of high iodine intake with the T1799A BRAF mutation in papillary thyroid cancer. J Clin Endocrinol Metab, 2009, 94(5):1612-1617.
11.	王玉龙, 王久存, 李端树, 等. 甲状腺乳头状癌的基因重排分析. 中华耳鼻咽喉头颈外科杂志, 2007, 42(12):929-933.
12.	于波洋, 杨盼, 马步云, 等. 甲状腺乳头状癌BRAF基因表达及侵袭性研究. 中国普外基础与临床杂志, 2015, 22(7):795-798.
13.	Zou M, Baitei EY, Alzahrani AS, et al. Concomitant RAS, RET/PTC, or BRAF mutations in advanced stage of papillary thyroid carcinoma. Thyroid, 2014, 24(8):1256-1266.
14.	Matsumoto H, Sakamoto A, Fujiwara M, et al. Cyclic AMP-mediated growth suppression and MAPK phosphorylation in thyroid papillary carcinoma cells. Mol Med Rep, 2011, 1(2):245-249.
15.	贾永胜. EGFR介导的MAPK再活化在BRAF^V600E基因突变的甲状腺乳头状癌中的作用研究. 第六届全国甲状腺肿瘤学术大会论文集. 天津:中国抗癌协会甲状腺肿瘤专业委员会, 中华医学会肿瘤分会头颈学组, 2014:23-25.
16.	Nan XL, Tamgüney TM, Collisson EA, et al. Ras-GTP dimers activate the mitogen-activated protein kinase (MAPK) pathway. Proc Natl Acad Sci USA, 2015, 112(26):7996-8001.
17.	Liu W, Ning R, Chen RN, et al. Aspafilioside B induces G2/M cell cycle arrest and apoptosis by up-regulating H-Ras and N-Ras via ERK and p38MAPK signaling pathways in human hepatoma HepG2 cells. Mol Carcinog, 2015 Feb 14. doi:10.1002/mc.22293.
18.	Nabti I, Marangos P, Bormann J, et al. Dual-mode regulation of the APC/C by CDK1 and MAPK controls meiosis Ⅰ progression and fidelity. J Cell Biol, 2014, 204(6):891-900.
19.	Myatt SS, Lam EW. The emerging roles of forkhead box (Fox) proteins in cancer. Nat Rev Cancer, 2007, 7(11):847-859.
20.	Ma RY, Tong TH, Cheung AM, et al. Raf/MEK/MAPK signaling stimulates the nuclear translocation and transactivating activity of FOXM1c. J Cell Sci, 2005, 118(Pt 4):795-806.
21.	Laoukili J, Alvarez M, Meijer LA, et al. Activation of FoxM1 during G2 requires cyclin A/Cdk-dependent relief of autorepression by the FoxM1 N-terminal domain. Mol Cell Biol, 2008, 28(9):3076-3087.
22.	Alvarez-Fernández M, Halim VA, Krenning L, et al. Recovery from a DNA-damage-induced G2 arrest requires Cdk-dependent activation of FoxM1. EMBO Rep, 2010, 11(6):452-458.
23.	Ahmed M, Uddin S, Hussain AR, et al. FoxM1 and its association with matrix metalloproteinases (MMP) signaling pathway in papillary thyroid carcinoma. J Clin Endocrinol Metab, 2012, 97(1):E1-E13.
24.	Bellelli R, Castellone MD, Garcia-Rostan G, et al. FOXM1 is a molecular determinant of the mitogenic and invasive phenotype of anaplastic thyroid carcinoma. Endocr Relat Cancer, 2012, 19(5):695-710.
25.	Kopanja D, Pandey A, Kiefer M, et al. Essential roles of FoxM1 in Ras-induced liver cancer progression and in cancer cells with stem cell features. J Hepatol, 2015, 63(2):429-436.

1. Occhi G, Barollo S, Regazzo D, et al. A constitutive active MAPK/ERK pathway due to BRAF^V600E positively regulates AHR pathway in PTC. Oncotarget, 2015, 6(31):32104-32114.
2. Davies L, Welch HG. Increasing incidence of thyroid cancer in the United States, 1973-2002. JAMA, 2006, 295(18):2164-2167.
3. Ramakrishna S, Kim IM, Petrovic V, et al. Myocardium defects and ventricular hypoplasia in mice homozygous null for the Forkhead Box M1 transcription factor. Dev Dyn, 2007, 236(4):1000-1013.
4. Brezillon N, Lambert-Blot M, Morosan S, et al. Transplanted hepato-cytes over-expressing FoxM1B efficiently repopulate chronically injured mouse liver independent of donor age. Mol Ther, 2007, 15(9):1710-1715.
5. Korver W, Roose J, Heinen K, et al. The human Trident/HFH-11/ FKHL16 gene:structure, localization, and promoter characterization. Genomics, 1997, 46(3):435-442.
6. Yau C, Meyer L, Benz S, et al. FOXM1 cistrome predicts breast cancer metastatic outcome better than FOXM1 expression levels or tumor proliferation index. Breast Cancer Res Treat, 2015, 154(1):23-32.
7. 王一茹, 姚斌伟, 张艳, 等. FoxM1 shRNA慢病毒载体构建及感染人前列腺癌细胞的稳定细胞株筛选. 南方医科大学学报, 2015, 35(9):1227-1233.
8. Zhang HG, Xu XW, Shi XP, et al. Overexpression of forkhead box protein M1 (FOXM1) plays a critical role in colorectal cancer. Clin Transl Oncol, 2015 Sep 14.
9. 袁浩, 陈进宏, 闵志均, 等. FoxM1基因表达与甲状腺乳头状癌TPC-1细胞活性及侵袭性关系的研究. 中华内分泌外科杂志, 2014, 7(4):265-268.
10. Guan H, Ji M, Bao R, et al. Association of high iodine intake with the T1799A BRAF mutation in papillary thyroid cancer. J Clin Endocrinol Metab, 2009, 94(5):1612-1617.
11. 王玉龙, 王久存, 李端树, 等. 甲状腺乳头状癌的基因重排分析. 中华耳鼻咽喉头颈外科杂志, 2007, 42(12):929-933.
12. 于波洋, 杨盼, 马步云, 等. 甲状腺乳头状癌BRAF基因表达及侵袭性研究. 中国普外基础与临床杂志, 2015, 22(7):795-798.
13. Zou M, Baitei EY, Alzahrani AS, et al. Concomitant RAS, RET/PTC, or BRAF mutations in advanced stage of papillary thyroid carcinoma. Thyroid, 2014, 24(8):1256-1266.
14. Matsumoto H, Sakamoto A, Fujiwara M, et al. Cyclic AMP-mediated growth suppression and MAPK phosphorylation in thyroid papillary carcinoma cells. Mol Med Rep, 2011, 1(2):245-249.
15. 贾永胜. EGFR介导的MAPK再活化在BRAF^V600E基因突变的甲状腺乳头状癌中的作用研究. 第六届全国甲状腺肿瘤学术大会论文集. 天津:中国抗癌协会甲状腺肿瘤专业委员会, 中华医学会肿瘤分会头颈学组, 2014:23-25.
16. Nan XL, Tamgüney TM, Collisson EA, et al. Ras-GTP dimers activate the mitogen-activated protein kinase (MAPK) pathway. Proc Natl Acad Sci USA, 2015, 112(26):7996-8001.
17. Liu W, Ning R, Chen RN, et al. Aspafilioside B induces G2/M cell cycle arrest and apoptosis by up-regulating H-Ras and N-Ras via ERK and p38MAPK signaling pathways in human hepatoma HepG2 cells. Mol Carcinog, 2015 Feb 14. doi:10.1002/mc.22293.
18. Nabti I, Marangos P, Bormann J, et al. Dual-mode regulation of the APC/C by CDK1 and MAPK controls meiosis Ⅰ progression and fidelity. J Cell Biol, 2014, 204(6):891-900.
19. Myatt SS, Lam EW. The emerging roles of forkhead box (Fox) proteins in cancer. Nat Rev Cancer, 2007, 7(11):847-859.
20. Ma RY, Tong TH, Cheung AM, et al. Raf/MEK/MAPK signaling stimulates the nuclear translocation and transactivating activity of FOXM1c. J Cell Sci, 2005, 118(Pt 4):795-806.
21. Laoukili J, Alvarez M, Meijer LA, et al. Activation of FoxM1 during G2 requires cyclin A/Cdk-dependent relief of autorepression by the FoxM1 N-terminal domain. Mol Cell Biol, 2008, 28(9):3076-3087.
22. Alvarez-Fernández M, Halim VA, Krenning L, et al. Recovery from a DNA-damage-induced G2 arrest requires Cdk-dependent activation of FoxM1. EMBO Rep, 2010, 11(6):452-458.
23. Ahmed M, Uddin S, Hussain AR, et al. FoxM1 and its association with matrix metalloproteinases (MMP) signaling pathway in papillary thyroid carcinoma. J Clin Endocrinol Metab, 2012, 97(1):E1-E13.
24. Bellelli R, Castellone MD, Garcia-Rostan G, et al. FOXM1 is a molecular determinant of the mitogenic and invasive phenotype of anaplastic thyroid carcinoma. Endocr Relat Cancer, 2012, 19(5):695-710.
25. Kopanja D, Pandey A, Kiefer M, et al. Essential roles of FoxM1 in Ras-induced liver cancer progression and in cancer cells with stem cell features. J Hepatol, 2015, 63(2):429-436.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Influence of FoxM1 Expression on Ras and CDK1 Expressions in Thyroid Papillary Carcinoma TPC-1 Cells

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