Protective Effect of Low-Molecular-Weight Heparin Calcium Combined with Trimetazi-dine on Intestinal Smooth Muscle of Intestinal Acute Mesangial Vein Thrombosis in Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 TUCan-neng ,  CHENJian-li , CHENJun-mao , WANGChang-you , WANGXiao-tao , ZHANGGuo-zhi

Department of General Surgery, The Affiliated Hospital, North China University of Science and Technology, Tangshan 063000, Hebei Province, China;

Corresponding author：

CHENJian-li, Email: mychenjianli@126.com

Keywords：

Acute mesenteric venous thrombosis; Intestinal smooth muscle; Low molecular weight heparin calcium; Trimetazidine

DOI：

10.7507/1007-9424.20160111

Video：

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Objective To explore the protective effect of low-molecular-weight heparin calcium (LHC) combined with trimetazidine on intestinal smooth muscle of intestinal acute mesangial vein thrombosis (AMVT) in rats and it's mechanism of effect. Methods A total of 120 SD male rats were randomly divided into three groups, with 40 rats in each group. LHC group: after the AMVT model established, rats were subcutaneous injection the LHC (30 U/100 g) per 12 h until 72 h after surgery. LHC+trimetazidine group (LHCT group): after the AMVT model established, rats were subcutaneous injection the LHC (30 U/100 g) and tail vein injection the trimetazidine (10 mg/kg) per 12 h until 72 h after surgery. Normal saline group (NS group): after the AMVT model established, rats were subcutaneous injection the NS (0.2 mL/100 g) per 12 h until 72 after surgery. The AMVT model were established by blocking superior mesenteric vein of 8 cm and the edge vein arch. Vena cava blood samples and intestinal segments were collected sequentially at 6 h, 12 h, 24 h, 48 h and 72 h afrer surgery. The levels of malondialdehyde (MDA) and creatine kinase (CK) in the blood, and the level of ATP in the intestinal tissue samples were measured with ELISA. Intestinal tissue were taken from the rats for inestinal tissue section, stained with hematoxylin and eosin, examined under light microscopy and evaluated histopathologically using mesemeche scoring system at different time. Results Compared with the LHC group and NS group, the levels of MDA and CK in blood and histopathology score of intestinal tissues in rats were significantly decreased, and the level of ATP significantly increased in LHCT group at different time point (P < 0.05). Conclusion Trimetazidine can improve intestinal smooth muscle energy metabolism in the AMVT disease, comined with LHC early can avoid intestinal smooth muscle wall permeability coagulation necrosis and reduce the intestinal smooth muscle damage.

Citation： TUCan-neng, CHENJian-li, CHENJun-mao, WANGChang-you, WANGXiao-tao, ZHANGGuo-zhi. Protective Effect of Low-Molecular-Weight Heparin Calcium Combined with Trimetazi-dine on Intestinal Smooth Muscle of Intestinal Acute Mesangial Vein Thrombosis in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(4): 416-420. doi: 10.7507/1007-9424.20160111 Copy

1.	陈建立, 刘彤, 戚峰.肠系膜上静脉血栓形成的实验及临床研究.天津医科大学, 2012, 81-82.
2.	Musemeche CA. A model of instinal ischemia in the neonatal rat utiling superior mesenteric artery occlusion and intraluminal plateletactivating factor. J surg Res, 1995, 58(6): 724-727.
3.	Hmoud B, Singal AK, Kamath PS. Mesenteric venous thrombosis. Clin Exp Hepatol, 2014, 4(3): 257-263.
4.	何文梅, 郭虹霞.急性肠系膜静脉血栓形成8例诊治分析.现代诊断与治疗, 2014, 25(22): 515-514.
5.	戈小虎, 朱峰.急性肠系膜静脉血栓形成的诊治进展.中国血管外科杂志:电子版, 2015, 7(4): 217-219.
6.	Acosta S, Krantz P. Trends in prevalence of fatal surgical diseases at forensic autopsy. ANZ J Surg, 2007, 77(9): 718-721.
7.	Condat B, Pessione F, Helene-Denninger M, et al. Recent portal or mesenteric venous thmmbosis:increased recognmon and fhquent recanalization on anticoagulant therapy. Hepatology, 2000, 32(3): 466-470．.
8.	许庆, 邓淼, 张栩, 等.急性肠系膜静脉血栓形成的临床诊治策略(附19例报告).临床和实验医学杂志, 2014, 13(13): 1099-1101.
9.	陈军, 夏培元, 常山, 等.烧伤血清对肠上皮细胞屏障功能损伤的实验研究.中华创伤杂志, 2002, 18(5):301-304.
10.	Krendel MF, Bonder EM. Analysis of actin filament bundle dynamics during contact formation in live epithelial cells. Cell Motil Cytoskeleton, 1999, 43(4): 296-309.
11.	张永珍, 杨立新, 阎西艴, 等.比索洛尔长程口服疗法改善冠心病病人的左室舒张功能不全.新药与临床, 1997, 16(5): 273-275.
12.	黄震华, 徐济民.尼卡地平治疗心绞痛.新药与临床, 1994, 13(3): 169-172.
13.	刘宏鸣, 樊志胜, 曹鲁鲁.地尔硫静脉滴注治疗冠心病优于丹参注射液.中国新药与临床杂志, 1998, 17(6): 355-357.
14.	Veitch K, maisin L, Hue L. Trimetazidine ef-fects on the Damage to Mitochondrial Function caused by ischemia and Reperfusion. Am J Cardiol, 1995, 76(6): 25B-30B.
15.	Broderick TL, Quinney HA, Barker CC, et al. Beneficial effect of carnitine on mechanical recovery of rat hearts reperfused after a transient period of global ischemia is accompanied by a stimulation of glucose oxidation. Circulation, 1993, 87(3): 972-981.
16.	Morin D, Elimadi A, Sapena R, et al. Evidence for the existence of [3H]-trimetazidine binding sites involved in the regulation of the mitochondrial permeability transition pore. Br J Pharmacol, 1998, 123(7): 1385-1394.
17.	Tselepis A, Doulias P, htrida E, et al. Tirmetazidine protects lowden sity lipoproteins from oxidation and cultured cells exposed to H₂O₂ from DNA damge. Free Radic Biol Med, 2001, 30(12): 1357-1364.
18.	Noble MI, belcheer PR. Drake-Holland AJ. Limitation of infarct size by trimetazidine in the rabbit. Am J Cardiol, 1995, 76(6):41B-44B.
19.	Demasion L, Fantini E, Sentex E, et al. Trimetazidine: in vitro influence on heart mitochodrial function. Am J Cardiol, 1995, 96(6): 31B-37B.
20.	Croall DE, DeMartino GN. Calcium-activated neutral protease (calpain) system: structure, function, and regulation. Physiol Rev, 1991, 71(3): 813-847.
21.	Beynon RJ, Bond JS. Catabolism of intracellular protein: molecular aspects. Am J Physiol, 1986, 251(2 Pt 1): C141-C152.
22.	Topham PS, Haydar SA, Kuphal R, et al. Complement-mediated injury reversibly disrupts glomerular epithelial cell actin microfila ments and focaladhesions. Kidney Int, 1999, 55(5): 1763-1775.
23.	顾忠平.曲美他嗪治疗缺血性心力衰竭的临床疗效观察.泰山医学院学报, 2014, 35(8): 798-799.
24.	杨明.曲美他嗪治疗劳累性心绞痛的疗效观察.中西医结合心血管病杂志, 2015, 3(7): 70-71.
25.	王健, 黄元伟, 魏经汉, 等.曲美他嗪对家兔心肌缺血再灌注损伤的保护作用.浙江大学校报, 2003, 32(3): 219-222.
26.	李选, 欧阳强, 萧湘生.肠系膜静脉血栓的介入治疗.介入放射学杂志, 2006, 15(4): 202-205.
27.	Rhee Ry, Gloviczki P, Mesenteric venous thrombosis. Surg Clin North am, 1997, 77(2): 327-338.
28.	Brunud L, Antunes L, Collinet-Adler S, et al. Acute mesenteric benous thrimbosis: case for nonopereative management. J Vasc surg, 2001, 34(4): 673-679.
29.	Kictchens CS. Evolution of our understanding of the pathophysio logy primary mesenteric venous thrombosis. Am J Surg, 1992, 163 (3): 346-348.

1. 陈建立, 刘彤, 戚峰.肠系膜上静脉血栓形成的实验及临床研究.天津医科大学, 2012, 81-82.
2. Musemeche CA. A model of instinal ischemia in the neonatal rat utiling superior mesenteric artery occlusion and intraluminal plateletactivating factor. J surg Res, 1995, 58(6): 724-727.
3. Hmoud B, Singal AK, Kamath PS. Mesenteric venous thrombosis. Clin Exp Hepatol, 2014, 4(3): 257-263.
4. 何文梅, 郭虹霞.急性肠系膜静脉血栓形成8例诊治分析.现代诊断与治疗, 2014, 25(22): 515-514.
5. 戈小虎, 朱峰.急性肠系膜静脉血栓形成的诊治进展.中国血管外科杂志:电子版, 2015, 7(4): 217-219.
6. Acosta S, Krantz P. Trends in prevalence of fatal surgical diseases at forensic autopsy. ANZ J Surg, 2007, 77(9): 718-721.
7. Condat B, Pessione F, Helene-Denninger M, et al. Recent portal or mesenteric venous thmmbosis:increased recognmon and fhquent recanalization on anticoagulant therapy. Hepatology, 2000, 32(3): 466-470．.
8. 许庆, 邓淼, 张栩, 等.急性肠系膜静脉血栓形成的临床诊治策略(附19例报告).临床和实验医学杂志, 2014, 13(13): 1099-1101.
9. 陈军, 夏培元, 常山, 等.烧伤血清对肠上皮细胞屏障功能损伤的实验研究.中华创伤杂志, 2002, 18(5):301-304.
10. Krendel MF, Bonder EM. Analysis of actin filament bundle dynamics during contact formation in live epithelial cells. Cell Motil Cytoskeleton, 1999, 43(4): 296-309.
11. 张永珍, 杨立新, 阎西艴, 等.比索洛尔长程口服疗法改善冠心病病人的左室舒张功能不全.新药与临床, 1997, 16(5): 273-275.
12. 黄震华, 徐济民.尼卡地平治疗心绞痛.新药与临床, 1994, 13(3): 169-172.
13. 刘宏鸣, 樊志胜, 曹鲁鲁.地尔硫静脉滴注治疗冠心病优于丹参注射液.中国新药与临床杂志, 1998, 17(6): 355-357.
14. Veitch K, maisin L, Hue L. Trimetazidine ef-fects on the Damage to Mitochondrial Function caused by ischemia and Reperfusion. Am J Cardiol, 1995, 76(6): 25B-30B.
15. Broderick TL, Quinney HA, Barker CC, et al. Beneficial effect of carnitine on mechanical recovery of rat hearts reperfused after a transient period of global ischemia is accompanied by a stimulation of glucose oxidation. Circulation, 1993, 87(3): 972-981.
16. Morin D, Elimadi A, Sapena R, et al. Evidence for the existence of [3H]-trimetazidine binding sites involved in the regulation of the mitochondrial permeability transition pore. Br J Pharmacol, 1998, 123(7): 1385-1394.
17. Tselepis A, Doulias P, htrida E, et al. Tirmetazidine protects lowden sity lipoproteins from oxidation and cultured cells exposed to H₂O₂ from DNA damge. Free Radic Biol Med, 2001, 30(12): 1357-1364.
18. Noble MI, belcheer PR. Drake-Holland AJ. Limitation of infarct size by trimetazidine in the rabbit. Am J Cardiol, 1995, 76(6):41B-44B.
19. Demasion L, Fantini E, Sentex E, et al. Trimetazidine: in vitro influence on heart mitochodrial function. Am J Cardiol, 1995, 96(6): 31B-37B.
20. Croall DE, DeMartino GN. Calcium-activated neutral protease (calpain) system: structure, function, and regulation. Physiol Rev, 1991, 71(3): 813-847.
21. Beynon RJ, Bond JS. Catabolism of intracellular protein: molecular aspects. Am J Physiol, 1986, 251(2 Pt 1): C141-C152.
22. Topham PS, Haydar SA, Kuphal R, et al. Complement-mediated injury reversibly disrupts glomerular epithelial cell actin microfila ments and focaladhesions. Kidney Int, 1999, 55(5): 1763-1775.
23. 顾忠平.曲美他嗪治疗缺血性心力衰竭的临床疗效观察.泰山医学院学报, 2014, 35(8): 798-799.
24. 杨明.曲美他嗪治疗劳累性心绞痛的疗效观察.中西医结合心血管病杂志, 2015, 3(7): 70-71.
25. 王健, 黄元伟, 魏经汉, 等.曲美他嗪对家兔心肌缺血再灌注损伤的保护作用.浙江大学校报, 2003, 32(3): 219-222.
26. 李选, 欧阳强, 萧湘生.肠系膜静脉血栓的介入治疗.介入放射学杂志, 2006, 15(4): 202-205.
27. Rhee Ry, Gloviczki P, Mesenteric venous thrombosis. Surg Clin North am, 1997, 77(2): 327-338.
28. Brunud L, Antunes L, Collinet-Adler S, et al. Acute mesenteric benous thrimbosis: case for nonopereative management. J Vasc surg, 2001, 34(4): 673-679.
29. Kictchens CS. Evolution of our understanding of the pathophysio logy primary mesenteric venous thrombosis. Am J Surg, 1992, 163 (3): 346-348.

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Protective Effect of Low-Molecular-Weight Heparin Calcium Combined with Trimetazi-dine on Intestinal Smooth Muscle of Intestinal Acute Mesangial Vein Thrombosis in Rats

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