低分子肝素钙对急性深静脉血栓大鼠血清IL-6和TNF-α水平的影响_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 李安强 , 史浩 , 牛启兵 ,  陈泉 , 董方

甘肃省人民医院血管外科（甘肃兰州 730000）;

Corresponding author：

陈泉, Email: 13919093907@163.com

Keywords：

低分子肝素钙; 深静脉血栓; 炎性反应

DOI：

10.7507/1007-9424.20160286

Video：

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目的探讨低分子肝素钙（LMWH）对急性下肢深静脉血栓（DVT）大鼠血清白介素6（IL-6）和肿瘤坏死因子α（TNF-α）水平的影响。方法将57只SD大鼠随机分为空白对照组20只和DVT组37只，DVT组予以结扎近心端股静脉，空白对照组大鼠不予结扎。再将DVT组大鼠随机分为实验对照组18只和LMWH组19只。空白对照组和实验对照组大鼠均给予2 mL生理盐水皮下注射，LMWH组大鼠给予2 mL LMWH皮下注射，每12小时注射1次，60 h后停止注射。分别于术前、术后36 h和术后72 h测量3组大鼠左下肢膝关节上6 mm处的左下肢周径及血清IL-6和TNF-α水平，并进行组间比较。结果术前空白对照组、实验对照组及LMWH组的左下肢周径、血清IL-6水平和血清TNF-α水平比较差异均无统计学意义（P＞0.050），但术后36 h和术后72 h 3组的左下肢周径、血清IL-6水平和血清TNF-α水平比较差异均有统计学意义（P＜0.050），均是实验对照组＞LMWH组＞空白对照组。结论急性下肢DVT SD大鼠行LMWH治疗后其血清IL-6和TNF-α水平均明显降低，提示LMWH对急性下肢DVT具有抗炎作用。

Citation： 李安强, 史浩, 牛启兵, 陈泉, 董方. 低分子肝素钙对急性深静脉血栓大鼠血清IL-6和TNF-α水平的影响. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(9): 1121-1124. doi: 10.7507/1007-9424.20160286 Copy

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15.	孙翠萍, 马建军, 李学, 等.无症状性脑梗死患者高敏C-反应蛋白、同型半胱氨酸、纤维蛋白原和血脂成分分析.中华实用诊断与治疗杂志, 2009, 23(1):32-34.
16.	Van Deventer SJ. Tumor necrosis factor and Crohn's disease. Gut, 1997, 40(4):443-448.
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19.	武梦骅.低分子肝素治疗新生儿脓毒症合并DIC 48例临床疗效观察.淮海医药, 2015, 33(5):182-183.

1. 党晓卫, 姚振涛, 乔师师, 等.兔下腔静脉球囊损伤后血管重塑相关因子的变化及G-CSF的干预作用.中国普通外科杂志, 2013, 22(6):721-725.
2. White RH. The epidemiology of venous thromboembolism. Circulation, 2003, 107(23 Suppl 1):14-18.
3. 关振鹏, 吕厚山.外科相关静脉血栓栓塞的防治.中国临床医生, 2009, 37(9):5-8.
4. 曹化, 居克举, 倪桂华, 等.弹力袜预防出血性卒中患者下肢深静脉血栓形成的有效性及安全性观察.中国全科医学, 2011, 14(3):333-335.
5. Lytvyn OI, Kopitsa MP, Petyunina OV. Interaction between inflammation and thrombosis in acute coronary syndrome. Kardiol Pol, 2004, 61(8):110-116.
6. Salobir B, Sabovic M. Possible vascular-bed-specific role of interleukin-6 in young women with a history of myocardial infarction, lacunar cerebral infarction and deep vein thrombosis. Cytokine, 2004, 25(6):265-272.
7. Esmon CT. The impact of the inflammatory response on coagulation. Thromb Res, 2004, 114(5):321-327.
8. van Aken BE, Reitsma PH, Rosendaal FR. Interleukin 8 and venous thrombosis:evidence for a role of inflammation in thrombosis. Br J Haematol, 2002, 116(1):173-177.
9. Kaplanski G, Fabrigoule M, Boulay V, et al. Thrombin induces endothelial type Ⅱ activation in vitro:IL-1 and TNF-alpha-independent IL-8 secretion and E-selectin expression. J Immunol, 1997, 158(11):5435-5441.
10. Wan MX, Zhang XW, Törkvist L, et al. Low molecular weight heparin inhibits tumor necrosis factor alpha-induced leukocyte rolling. Inflamm Res, 2001, 50(12):581-584.
11. Celasco G, Moro L, Bozzella R, et al. Efficacy of intracolonic administration of low-molecular-weight heparin CB-01-05, compared to other low-molecular-weight heparins and unfractionated heparin, in experimentally induced colitis in rat. Dig Dis Sci, 2008, 53(12):3170-3175.
12. Papa A, Danese S, Gasbarrini A, et al. Review article:potential therapeutic applications and mechanisms of action of heparin in inflammatory bowel disease. Aliment Pharmacol Ther, 2000, 14(11):1403-1409.
13. Otsuka M, Tsuchiya S, Aramaki Y. Comparison of inhibitory effects of polyanions on nitric oxide production by macrophages stimulated with LPS. Biol Pharm Bull, 2006, 29(3):499-502.
14. Salas A, Sans M, Soriano A, et al. Heparin attenuates TNF-alpha induced inflammatory response through a CD11b dependent mechanism. Gut, 2000, 47(1):88-96.
15. 孙翠萍, 马建军, 李学, 等.无症状性脑梗死患者高敏C-反应蛋白、同型半胱氨酸、纤维蛋白原和血脂成分分析.中华实用诊断与治疗杂志, 2009, 23(1):32-34.
16. Van Deventer SJ. Tumor necrosis factor and Crohn's disease. Gut, 1997, 40(4):443-448.
17. Backer S, Derlin RB, Haskill JS. Differential production of tumor necrosis factor macrophage colony stimulating factor and interleukin-1 by human alveolar macrophages. J Leukol Biol, 1989, 45(4):353-361.
18. 林春繁.低分子肝素治疗新生儿脓毒症并弥散性血管内凝血的临床研究.国际医药卫生导报, 2014, 20(1):55-57.
19. 武梦骅.低分子肝素治疗新生儿脓毒症合并DIC 48例临床疗效观察.淮海医药, 2015, 33(5):182-183.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

低分子肝素钙对急性深静脉血栓大鼠血清IL-6和TNF-α水平的影响

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