The Expressions and Significance of TypeⅠand Type Ⅲ Collagen Protein in Patients with Hepatic Alveolar Echinococcosis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 YANGDANCairang ^1,2 , SONGGe ^1,2 , ZHANGLing-qiang ^1,2 , YOUZhi-yuan ^1,2 , RENLi ^1,2 , HOULi-chao ^1,2 , WANGHai-jiu ^1,2 , ZHOUYing ^1,2 , WENHao ³ ,  FANHai-ning ^1,2

1. Department of Hepatopancreatobiliary Surgery, The Affiliated Hospital of Qinghai University, Xining 810001, Qinghai Province, China;
2. The Key Laboratory of Hydatid Disease Research in Qinghai Province, Xining 810001, Qinghai Province, China;
3. The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China;

Corresponding author：

FANHai-ning, Email: fanhaining@medmail.com.cn

Keywords：

Hepatic alveolar echinococcosis; Echinococcus multilocularis; Collogen protein; Hepatic fibrosis

DOI：

10.7507/1007-9424.20160310

Video：

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Objective To investigate the expressions of type Ⅰ and type Ⅲ collagen protein in hepatic alveolar echinococcosis tissues, and to explore its relationship with the biological behavior in progress of hepatic alveolar echinococcosis (HAE). Methods Twenty samples of normal liver tissues and liver tissues at the edge of the lesion with HAE in our hospital from Jan. 2012 to Dec. 2014 were collected, and HE and Masson staining were performed. The pathological changes and the degree of fibrosis of liver tissues around HAE lesion were observed under light microscope. The expressions of type Ⅰ and type Ⅲ collagen protein in liver tissues were detected by immunohistochemical staining. Results The degree of liver fibrosis of liver tissues at the edge of the lesion with HAE was grade Ⅱ, and the degree of fibrosis of normal liver tissues was grade 0, the difference between the two was statistically significant (P < 0.05). The color index of type Ⅰand type Ⅲ collagen protein in the liver tissues at the edge of the lesion with HAE was 7.45±1.85 and 8.00±1.62, respectively, which were higher than those of normal liver tissues (3.10±1.02 and 3.50±0.89), the difference were statistically significant (t=-9.21, P=0.001;t=-10.88, P=0.001). Conclusions There is liver fibrosis around the lesion in the patients with HAE. HAE may promote the expressions of type Ⅰ and type Ⅲ collagen and then induce the occurrence of liver fibrosis.

Citation： YANGDAN Cairang, SONG Ge, ZHANG Ling-qiang, YOU Zhi-yuan, REN Li, HOU Li-chao, WANG Hai-jiu, ZHOU Ying, WEN Hao, FAN Hai-ning. The Expressions and Significance of TypeⅠand Type Ⅲ Collagen Protein in Patients with Hepatic Alveolar Echinococcosis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(10): 1209-1213. doi: 10.7507/1007-9424.20160310 Copy

1.	Vuitton DA, Zhou H, Bresson-Hadni S, et al. Epidemiology of alveolar echinococcosis with particular reference to China and Europe. Parasitology, 2003, 127 Suppl:87-107.
2.	路越晴, 邓勇, 张灵强, 等. 泡型肝包虫病的临床治疗进展. 中国普外基础与临床杂志, 2016, 23(5):547-550.
3.	侯玉虎, 马淑梅, 樊海宁, 等. 青海达日、班玛地区小学生肝包虫病患病情况调查. 现代预防医学, 2016, 43(13):2363-2365, 2381.
4.	张昀昊, 任利, 阳丹才让, 等. 肝泡型包虫病根治性切除163例回顾性分析. 中国普通外科杂志, 2016, 25(2):257-263.
5.	刘平, 李金花, 李印, 等. 包虫病病原在我国的流行现状及成因分析. 中国动物检疫, 2016, 23(1):48-51.
6.	Lee UE, Friedman SL. Mechanisms of hepatic fibrogenesis. Best Pract Res Clin Gastroenterol, 2011, 25(2):195-206.
7.	吴向未, 陈雪玲, 彭心宇, 等. Ⅰ、Ⅲ、Ⅳ型胶原在肝包虫囊肿周围人体纤维囊壁中的特异性分层表达. 中国人兽共患病学报, 2004, 20(10):876-879.
8.	王俊华, 魏绪法, 张传山, 等. Ⅰ、Ⅲ、Ⅳ胶原基因在泡球蚴感染中期小鼠肝脏中的表达及意义. 中国病原生物学杂志, 2012, 7(9):653-656.
9.	吴晓玲, 曾维政, 蒋明德, 等. 肝纤维化的信号转导通路. 世界华人消化杂志, 2006, 14(22):2223-2228.
10.	杨艳宏, 蔡卫民, 金国梁, 等. 日本血吸虫感染兔肝肌成纤维细胞的动态变化及其意义. 中华医学杂志, 1999, 79(11):870-873.
11.	Mattern J, Stammler G, Koomagi R, et al. Association of vascular endothelial growth factor expression with tumor cell proliferation in ovarian carcinoma. Anticancer Res, 1997, 17(17):621-624.
12.	Chauchet A, Grenouillet F, Knapp J, et al. Increased incidence and characteristics of alveolar echinococcosis in patients with immunosuppression-associated conditions. Clin Infect Dis, 2014, 59(8):1095-1104.
13.	Wang J, Gottstein B. Immunoregulation in larval Echinococcus multilocularis, infection. Parasite Immunol, 2016, 38(3):182-192.
14.	Zhang F, Pang N, Zhu Y, et al. CCR7^lo PD-1^hi, CXCR5⁺, CD4⁺, T cells are positively correlated with levels of IL-21 in active and transitional cystic echinococcosis patients. BMC Infect Dis, 2015, 15(1):1-11.
15.	张梦然, 成军. 肝纤维化研究进展. 国际消化病杂志, 2014,34(6):374-379.
16.	张斌. 肝纤维化的发病机理研究进展. 现代诊断与治疗, 2004, 15(6):354-359.
17.	李伟道. 诊断肝纤维化、肝硬变的新进展:血清中细胞外基质成份的测定. 中国普外基础与临床杂志, 1997, 4(1):37-39.
18.	王要军, 孙自勤. 肝窦内皮细胞与肝纤维化. 临床肝胆病杂志, 1996, 12(3):115-117.
19.	Ryhanen L, Stenback F, Ala-Kokko L, et al. The effect of malotilate on type Ⅲ and type Ⅳ collagen, laminin and fibronectin metabolism in dimethylnitrosamine-induced liver fibrosis in the rat. J Hepatol, 1996, 24(2):238-245.
20.	Nakayama Y, Takahara T, Miyabayashi C, et al. Ultrastructural localization of type Ⅳcollagen and laminin in the Disse space of rat liver with carbon tetrachloride induced fibrosis. Liver, 1991, 11(5):260-271.
21.	Murawaki Y, Ikuta Y, Koda M, et al. Comparison of serum 7S fragment of type Ⅳ collagen and serum central triple-helix of type Ⅳ collagen for assessment of liver fibrosis in patients with chronic viral liver disease. J Hepatol, 1996, 24(2):148-154.
22.	Tsutsumi M, Takase S, Urashima S, et al. Serum markers for hepatic fibrosis in alcoholic liver disease:which is the best marker, type Ⅲ procollagen, type Ⅳ collagen, laminin, tissue inhibitor of metalloproteinase, or prolyl hydroxylase? Alcohol Clin Exp Res, 1996, 20(9):1512-1517.
23.	陈光文, 宋彬. 非侵入性方法评价肝纤维化. 中国普外基础与临床杂志, 2009, 16(5):414-417.
24.	Macwell PR, Flisiak R. Changes in serological biomarkers of liver funchtion and connective tissue turnover in chronic hepatitis B during lamivudine therapy. Biomarkerds, 2005, 10(6):475-484.
25.	Vuitton DA, Guerret-Stocker S, Carbillet JP, et al. Collagen immunotyping of the hepatic fibrosis in human alveolar echinococcosis. Z Parasitenkd, 1986, 72(1):97-104.
26.	Dar FK, Alkarmi T. 泡球蚴病的免疫病理学. 国际医学寄生虫病杂志, 1998, 25(6):270-272.

1. Vuitton DA, Zhou H, Bresson-Hadni S, et al. Epidemiology of alveolar echinococcosis with particular reference to China and Europe. Parasitology, 2003, 127 Suppl:87-107.
2. 路越晴, 邓勇, 张灵强, 等. 泡型肝包虫病的临床治疗进展. 中国普外基础与临床杂志, 2016, 23(5):547-550.
3. 侯玉虎, 马淑梅, 樊海宁, 等. 青海达日、班玛地区小学生肝包虫病患病情况调查. 现代预防医学, 2016, 43(13):2363-2365, 2381.
4. 张昀昊, 任利, 阳丹才让, 等. 肝泡型包虫病根治性切除163例回顾性分析. 中国普通外科杂志, 2016, 25(2):257-263.
5. 刘平, 李金花, 李印, 等. 包虫病病原在我国的流行现状及成因分析. 中国动物检疫, 2016, 23(1):48-51.
6. Lee UE, Friedman SL. Mechanisms of hepatic fibrogenesis. Best Pract Res Clin Gastroenterol, 2011, 25(2):195-206.
7. 吴向未, 陈雪玲, 彭心宇, 等. Ⅰ、Ⅲ、Ⅳ型胶原在肝包虫囊肿周围人体纤维囊壁中的特异性分层表达. 中国人兽共患病学报, 2004, 20(10):876-879.
8. 王俊华, 魏绪法, 张传山, 等. Ⅰ、Ⅲ、Ⅳ胶原基因在泡球蚴感染中期小鼠肝脏中的表达及意义. 中国病原生物学杂志, 2012, 7(9):653-656.
9. 吴晓玲, 曾维政, 蒋明德, 等. 肝纤维化的信号转导通路. 世界华人消化杂志, 2006, 14(22):2223-2228.
10. 杨艳宏, 蔡卫民, 金国梁, 等. 日本血吸虫感染兔肝肌成纤维细胞的动态变化及其意义. 中华医学杂志, 1999, 79(11):870-873.
11. Mattern J, Stammler G, Koomagi R, et al. Association of vascular endothelial growth factor expression with tumor cell proliferation in ovarian carcinoma. Anticancer Res, 1997, 17(17):621-624.
12. Chauchet A, Grenouillet F, Knapp J, et al. Increased incidence and characteristics of alveolar echinococcosis in patients with immunosuppression-associated conditions. Clin Infect Dis, 2014, 59(8):1095-1104.
13. Wang J, Gottstein B. Immunoregulation in larval Echinococcus multilocularis, infection. Parasite Immunol, 2016, 38(3):182-192.
14. Zhang F, Pang N, Zhu Y, et al. CCR7^lo PD-1^hi, CXCR5⁺, CD4⁺, T cells are positively correlated with levels of IL-21 in active and transitional cystic echinococcosis patients. BMC Infect Dis, 2015, 15(1):1-11.
15. 张梦然, 成军. 肝纤维化研究进展. 国际消化病杂志, 2014,34(6):374-379.
16. 张斌. 肝纤维化的发病机理研究进展. 现代诊断与治疗, 2004, 15(6):354-359.
17. 李伟道. 诊断肝纤维化、肝硬变的新进展:血清中细胞外基质成份的测定. 中国普外基础与临床杂志, 1997, 4(1):37-39.
18. 王要军, 孙自勤. 肝窦内皮细胞与肝纤维化. 临床肝胆病杂志, 1996, 12(3):115-117.
19. Ryhanen L, Stenback F, Ala-Kokko L, et al. The effect of malotilate on type Ⅲ and type Ⅳ collagen, laminin and fibronectin metabolism in dimethylnitrosamine-induced liver fibrosis in the rat. J Hepatol, 1996, 24(2):238-245.
20. Nakayama Y, Takahara T, Miyabayashi C, et al. Ultrastructural localization of type Ⅳcollagen and laminin in the Disse space of rat liver with carbon tetrachloride induced fibrosis. Liver, 1991, 11(5):260-271.
21. Murawaki Y, Ikuta Y, Koda M, et al. Comparison of serum 7S fragment of type Ⅳ collagen and serum central triple-helix of type Ⅳ collagen for assessment of liver fibrosis in patients with chronic viral liver disease. J Hepatol, 1996, 24(2):148-154.
22. Tsutsumi M, Takase S, Urashima S, et al. Serum markers for hepatic fibrosis in alcoholic liver disease:which is the best marker, type Ⅲ procollagen, type Ⅳ collagen, laminin, tissue inhibitor of metalloproteinase, or prolyl hydroxylase? Alcohol Clin Exp Res, 1996, 20(9):1512-1517.
23. 陈光文, 宋彬. 非侵入性方法评价肝纤维化. 中国普外基础与临床杂志, 2009, 16(5):414-417.
24. Macwell PR, Flisiak R. Changes in serological biomarkers of liver funchtion and connective tissue turnover in chronic hepatitis B during lamivudine therapy. Biomarkerds, 2005, 10(6):475-484.
25. Vuitton DA, Guerret-Stocker S, Carbillet JP, et al. Collagen immunotyping of the hepatic fibrosis in human alveolar echinococcosis. Z Parasitenkd, 1986, 72(1):97-104.
26. Dar FK, Alkarmi T. 泡球蚴病的免疫病理学. 国际医学寄生虫病杂志, 1998, 25(6):270-272.

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

The Expressions and Significance of TypeⅠand Type Ⅲ Collagen Protein in Patients with Hepatic Alveolar Echinococcosis

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