Experimental Research of Laryngeal Muscle and Pathophysiology of Recurrent Laryngeal Nerve after Unilateral Recurrent Laryngeal Nerve Transection for Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 QUJun , SHENYe , WANGWen-jie , HUANGZhong-ming , WANGShi-guang ,  WUTao

Department of General Surgery, Shanghai Fengxian District Central Hospital, Shanghai Jiao Tong University Affiliated Sixth People's Hospital South Campus, Shanghai 201499, China;

Corresponding author：

WUTao, Email: hijump46@sohu.com

Keywords：

Recurrent laryngeal nerve; Transection; Brain-derived neurotrophic factor; Vocal cord palsy; Nerve regeneration; Rat

DOI：

10.7507/1007-9424.20160366

Video：

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Objective The aim of this study was to evaluate the repair effect of spontaneous reinnervation in rats underwent recurrent laryngeal nerve (RLN) transection. Methods Thirty male Wistar rats (340-360 g) were divided into experiment group (n=15) and blank control group (n=15), and then 15 rats of these 2 groups were divided into 3 time point groups equally:4 weeks group, 8 weeks group, and 12 weeks group. Fifteen rats of experiment group underwent right RLN transection with excision of a 5 mm segment, and other 15 rats of blank control group exposed RLN only, without transection. Grade of vocalization, maximum angle of arytenoid cartilage, axon number of distal part of RLN, and expression of the brain-derived neurotrophic factor (BDNF) in right thyroarytenoid muscle were evaluated at different time points, including 4, 8, and 12 weeks after operation. Results Grade of vocalization, maximum angle of arytenoid cartilage, axon numbers of distal part of RLN, and the expression of BDNF in the right thyroarytenoid muscle of experiment group were all lower than those corresponding index of blank control group (P < 0.05), and these indexes of experiment group were restored gradually with time, but failed to reach normal level during the observed time. Conclusions Even though spontaneous reinnervation is presented after RLN injury, but the effect is unsatisfactory.

Citation： QUJun, SHENYe, WANGWen-jie, HUANGZhong-ming, WANGShi-guang, WUTao. Experimental Research of Laryngeal Muscle and Pathophysiology of Recurrent Laryngeal Nerve after Unilateral Recurrent Laryngeal Nerve Transection for Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(12): 1437-1442. doi: 10.7507/1007-9424.20160366 Copy

1.	Brown TJ, Pittman AL, Monaco GN, et al. Androgen treatment and recovery of function following recurrent laryngeal nerve injury in the rat. Restor Neurol Neurosci, 2013, 31(2):169-176.
2.	Moro K, Shiotani A, Watabe K, et al. Adenoviral gene transfer of BDNF and GDNF synergistically prevent motoneuron loss in the nucleus ambiguus. Brain Res, 2006, 1076(1):1-8.
3.	Miyamaru S, Kumai Y, Ito T, et al. Effects of long-term denervation on the rat thyroarytenoid muscle. Laryngoscope, 2008, 118(7):1318-1323.
4.	Fang TJ, Li HY, Gliklich RE, et al. Quality of life measures and predictors for adults with unilateral vocal cord paralysis. Laryngoscope, 2008, 118(10):1837-1841.
5.	冯云, 杨大章, 吕秋萍, 等.喉返神经修复术及非喉返神经修复术治疗声带麻痹.山东大学耳鼻喉眼学报, 2015, 29(3):54-58.
6.	Araki K, Shiotani A, Watabe K, et al. Adenoviral GDNF gene transfer enhances neurofunctional recovery after recurrent laryngeal nerve injury. Gene Ther, 2006, 13(4):296-303.
7.	王保鑫, 董频.神经营养因子对喉返神经损伤再生的研究进展.中国眼耳鼻喉科杂志, 2014, 14(1):52-55.
8.	李孟, 陈世彩, 陈东辉, 等.肝细胞生长因子对喉返神经损伤后再生的作用.听力学及言语疾病杂志, 2012, 20(5):467-471.
9.	Levi-Montalcini R. The nerve growth factor 35 years later. Science, 1987, 237(4819):1154-1162.
10.	曾维军, 周文胜, 王佳.神经营养因子和生长因子在神经干细胞中的研究进展.临床与病理杂志, 2015, 35(3):484-489.
11.	Han Q, Jin W, Xiao Z, et al. The promotion of neural regeneration in an extreme rat spinal cord injury model using a collagen scaffold containing a collagen binding neuroprotective protein and an EGFR neutralizing antibody. Biomaterials, 2010, 31(35):9212-9220.
12.	何双八, 孙敬武, 孙东东.单侧喉返神经切断后甲杓肌结构与酶学变化的实验性研究.安徽医科大学学报, 2005, 40(3):231-233.
13.	徐文, 赵功伟, 胡慧英, 等.喉返神经损伤后喉肌病理生理研究.临床耳鼻咽喉头颈外科杂志, 2009, 23(9):403-406.
14.	Vega-Cordova X, Cosenza NM, Helfert RH, et al. Neurotrophin expression of laryngeal muscles in response to recurrent laryngeal nerve transection. Laryngoscope, 2010, 120(8):1591-1596.
15.	施剑斌, 郑宏良, 陈世彩, 等.改良杓状软骨内收术治疗单侧声带麻痹.听力学及言语疾病杂志, 2010, 18(1):29-32.
16.	张品一, 张滨, 刘垚, 等.甲状腺手术中喉返神经变异的临床研究.中华普通外科杂志, 2013, 28(6):431-435.
17.	申曦, 李小毅, 刘跃武, 等.非返性喉返神经的解剖特点及术中损伤的预防.中国普外基础与临床杂志, 2013, 20(1):71-76.
18.	Zábrodský M, Bouček J, Kastner J, et al. Immediate revision in patients with bilateral recurrent laryngeal nerve palsy after thyroid and parathyroid surgery. How worthy is it? Acta Otorhinolaryngol Ital, 2012, 32(4):222-228.
19.	Sunderland S. A classification of peripheral nerve injuries producing loss of function. Brain, 1951, 74(4):491-516.
20.	Woodson GE, Hughes LF, Helfert R. Quantitative assessment of laryngeal muscle morphology after recurrent laryngeal nerve injury:right vs. left differences. Laryngoscope, 2008, 118(10):1768-1770.
21.	侯丽珍, 韩德民, 徐文, 等.喉返神经麻痹的肌电特点及相关研究.临床耳鼻咽喉头颈外科杂志, 2007, 21(15):673-678.
22.	马情情, 孙丽, 郑宏良, 等.特发性声带麻痹患者环杓后肌肌纤维形态观察.听力学及言语疾病杂志, 2015, 23(3):256-260.
23.	Aoyama T, Kumai Y, Yumoto E, et al. Effects of nerve-muscle pedicle on immobile rat vocal folds in the presence of partial innervation. Ann Otol Rhinol Laryngol, 2010, 119(12):823-829.
24.	Pitman MJ, Weissbrod P, Roark R, et al. Electromyographic and histologic evolution of the recurrent laryngeal nerve from transection and anastomosis to mature reinnervation. Laryngoscope, 2011, 121(2):325-331.
25.	Toya Y, Kumai Y, Minoda R, et al. Modulation of nerve fibers in the rat thyroarytenoid muscle following recurrent laryngeal nerve injury. Acta Otolaryngol, 2012, 132(3):305-313.
26.	Sharma N, Marzo SJ, Jones KJ, et al. Electrical stimulation and testosterone differentially enhance expression of regeneration-associated genes. Exp Neurol, 2010, 223(1):183-191.

1. Brown TJ, Pittman AL, Monaco GN, et al. Androgen treatment and recovery of function following recurrent laryngeal nerve injury in the rat. Restor Neurol Neurosci, 2013, 31(2):169-176.
2. Moro K, Shiotani A, Watabe K, et al. Adenoviral gene transfer of BDNF and GDNF synergistically prevent motoneuron loss in the nucleus ambiguus. Brain Res, 2006, 1076(1):1-8.
3. Miyamaru S, Kumai Y, Ito T, et al. Effects of long-term denervation on the rat thyroarytenoid muscle. Laryngoscope, 2008, 118(7):1318-1323.
4. Fang TJ, Li HY, Gliklich RE, et al. Quality of life measures and predictors for adults with unilateral vocal cord paralysis. Laryngoscope, 2008, 118(10):1837-1841.
5. 冯云, 杨大章, 吕秋萍, 等.喉返神经修复术及非喉返神经修复术治疗声带麻痹.山东大学耳鼻喉眼学报, 2015, 29(3):54-58.
6. Araki K, Shiotani A, Watabe K, et al. Adenoviral GDNF gene transfer enhances neurofunctional recovery after recurrent laryngeal nerve injury. Gene Ther, 2006, 13(4):296-303.
7. 王保鑫, 董频.神经营养因子对喉返神经损伤再生的研究进展.中国眼耳鼻喉科杂志, 2014, 14(1):52-55.
8. 李孟, 陈世彩, 陈东辉, 等.肝细胞生长因子对喉返神经损伤后再生的作用.听力学及言语疾病杂志, 2012, 20(5):467-471.
9. Levi-Montalcini R. The nerve growth factor 35 years later. Science, 1987, 237(4819):1154-1162.
10. 曾维军, 周文胜, 王佳.神经营养因子和生长因子在神经干细胞中的研究进展.临床与病理杂志, 2015, 35(3):484-489.
11. Han Q, Jin W, Xiao Z, et al. The promotion of neural regeneration in an extreme rat spinal cord injury model using a collagen scaffold containing a collagen binding neuroprotective protein and an EGFR neutralizing antibody. Biomaterials, 2010, 31(35):9212-9220.
12. 何双八, 孙敬武, 孙东东.单侧喉返神经切断后甲杓肌结构与酶学变化的实验性研究.安徽医科大学学报, 2005, 40(3):231-233.
13. 徐文, 赵功伟, 胡慧英, 等.喉返神经损伤后喉肌病理生理研究.临床耳鼻咽喉头颈外科杂志, 2009, 23(9):403-406.
14. Vega-Cordova X, Cosenza NM, Helfert RH, et al. Neurotrophin expression of laryngeal muscles in response to recurrent laryngeal nerve transection. Laryngoscope, 2010, 120(8):1591-1596.
15. 施剑斌, 郑宏良, 陈世彩, 等.改良杓状软骨内收术治疗单侧声带麻痹.听力学及言语疾病杂志, 2010, 18(1):29-32.
16. 张品一, 张滨, 刘垚, 等.甲状腺手术中喉返神经变异的临床研究.中华普通外科杂志, 2013, 28(6):431-435.
17. 申曦, 李小毅, 刘跃武, 等.非返性喉返神经的解剖特点及术中损伤的预防.中国普外基础与临床杂志, 2013, 20(1):71-76.
18. Zábrodský M, Bouček J, Kastner J, et al. Immediate revision in patients with bilateral recurrent laryngeal nerve palsy after thyroid and parathyroid surgery. How worthy is it? Acta Otorhinolaryngol Ital, 2012, 32(4):222-228.
19. Sunderland S. A classification of peripheral nerve injuries producing loss of function. Brain, 1951, 74(4):491-516.
20. Woodson GE, Hughes LF, Helfert R. Quantitative assessment of laryngeal muscle morphology after recurrent laryngeal nerve injury:right vs. left differences. Laryngoscope, 2008, 118(10):1768-1770.
21. 侯丽珍, 韩德民, 徐文, 等.喉返神经麻痹的肌电特点及相关研究.临床耳鼻咽喉头颈外科杂志, 2007, 21(15):673-678.
22. 马情情, 孙丽, 郑宏良, 等.特发性声带麻痹患者环杓后肌肌纤维形态观察.听力学及言语疾病杂志, 2015, 23(3):256-260.
23. Aoyama T, Kumai Y, Yumoto E, et al. Effects of nerve-muscle pedicle on immobile rat vocal folds in the presence of partial innervation. Ann Otol Rhinol Laryngol, 2010, 119(12):823-829.
24. Pitman MJ, Weissbrod P, Roark R, et al. Electromyographic and histologic evolution of the recurrent laryngeal nerve from transection and anastomosis to mature reinnervation. Laryngoscope, 2011, 121(2):325-331.
25. Toya Y, Kumai Y, Minoda R, et al. Modulation of nerve fibers in the rat thyroarytenoid muscle following recurrent laryngeal nerve injury. Acta Otolaryngol, 2012, 132(3):305-313.
26. Sharma N, Marzo SJ, Jones KJ, et al. Electrical stimulation and testosterone differentially enhance expression of regeneration-associated genes. Exp Neurol, 2010, 223(1):183-191.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Experimental Research of Laryngeal Muscle and Pathophysiology of Recurrent Laryngeal Nerve after Unilateral Recurrent Laryngeal Nerve Transection for Rats

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