泡球蚴囊液对大鼠肝星状细胞TGF-β1、IL-6及TNF-α表达的影响_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 李衍飞 ^1,2 , 邵军 ³ , 王志鑫 ^1,2 , 姚宝洪 ³ , 阳丹才让 ^1,2 , 任利 ^1,2 , 候立朝 ^1,2 , 王海久 ^1,2 , 周瀛 ^1,2 , 温浩 ⁴ , 王虎 ⁵ ,  樊海宁 ^1,2

1. ;
2. ;
3. ;
4. ;
5. ;

Corresponding author：

樊海宁, Email: fanhaining@medmail.com.cn

Keywords：

泡球蚴囊液; 肝星状细胞; 转化生长因子β1; 白细胞介素6; 肿瘤坏死因子-α

DOI：

10.7507/1007-9424.20160378

Video：

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目的观察泡球蚴囊液对大鼠肝星状细胞（HSC-T6）中转化生长因子β1（TGF-β1）、白细胞介素6（IL-6）及肿瘤坏死因子-α（TNF-α）表达的影响，以初步揭示泡型肝包虫病免疫调节作用的潜在机理。方法以泡球蚴囊液中蛋白成分的浓度代表囊液浓度，分别以0、0.01、0.025、0.05、0.1、0.2、0.4、0.9、1.7、3.4、6.8及13.5 mg/mL的泡球蚴囊液干预大鼠HSC-T6细胞，作用24 h后收集各组细胞上清，采用酶联免疫吸附法检测上清中TGF-β1、IL-6及TNF-α的浓度。结果以不同浓度泡球蚴囊液（0~13.5 mg/mL）干预后，HSC-T6细胞培养液上清中的TGF-β1浓度几乎不变，各浓度组间两两比较差异均无统计学意义（P＞0.05）。当泡球蚴囊液浓度≤3.4 mg/mL时，上清中IL-6的浓度趋于平稳，各浓度组间两两比较差异均无统计学意义（P＞0.05）；当泡球蚴囊液浓度为6.8和13.5 mg/mL时，IL-6浓度较0 mg/mL组均升高，差异均有统计学意义（P＜0.01）。当泡球蚴囊液浓度≤0.2 mg/mL时，上清中的TNF-α浓度趋于平稳，各浓度组间两两比较差异均无统计学意义（P＞0.05）；当泡球蚴囊液浓度为0.4 mg/mL时，TNF-α浓度达到最大，为0 mg/mL组的3.53倍（P＜0.05）；此后随浓度增高TNF-α浓度又逐渐降低，但仍高于0 mg/mL组（P＜0.05）。结论 IL-6和TNF-α可能在肝星状细胞介导的泡型肝包虫病的纤维化和组织损伤中扮演重要角色，而TGF-β1的作用尚不确定。

Citation： 李衍飞, 邵军, 王志鑫, 姚宝洪, 阳丹才让, 任利, 候立朝, 王海久, 周瀛, 温浩, 王虎, 樊海宁. 泡球蚴囊液对大鼠肝星状细胞TGF-β1、IL-6及TNF-α表达的影响. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(12): 1500-1502. doi: 10.7507/1007-9424.20160378 Copy

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18.	王俊华, 卢晓梅, 张传山, 等.转化生长因子-β1及凋亡相关因子在泡球蚴感染宿主病灶旁免疫细胞中的表达及其意义.中华实验外科杂志, 2011, 28(6):929-931.
19.	Hemeda H, Jakob M, Ludwig AK, et al. Interferon-gamma and tumor necrosis factor-alpha differentially affect cytokine expression and migration properties of mesenchymal stem cells. Stem Cells Dev, 2010, 19(5):693-706.
20.	Li HY, Ju D, Zhang DW, et al. Activation of TGF-β1-CD147 positive feedback loop in hepatic stellate cells promotes liver fibrosis. Sci Rep, 2015, 5:16552.
21.	Yu Y, Lu L, Qian X, et al. Antifibrotic effect of hepatocyte growth factor-expressing mesenchymal stem cells in small-for-size liver transplant rats. Stem Cells Dev, 2010, 19(6):903-914.
22.	Parekkadan B, van Poll D, Megeed Z, et al. Immunomodulation of activated hepatic stellate cells by mesenchymal stem cells. Biochem Biophys Res Commun, 2007, 363(2):247-252.
23.	Friedman SL. Hepatic fibrosis-overview. Toxicology, 2008, 254(3):120-129.
24.	李婧婷, 汪晖, 廖长秀, 等.肝星状细胞激活的分子机制与抗肝纤维化治疗.武汉大学学报:医学版, 2007, 28(2):256-261.

1. 赵慧, 白雪, 聂晓涵, 等.泡球蚴感染小鼠肝脏中IL-10和TGF-β1的动态变化.中国寄生虫学与寄生虫病杂志, 2012, 30(1):32-35.
2. Parsons CJ, Takashima M, Rippe RA. Molecular mechanisms of hepatic fibrogenesis. J Gastroenterol Hepatol, 2007, 22 Suppl 1:S79-S84.
3. Craig PS, Li T, Qiu J, et al. Echinococcosis and Tibetan communities. Emerg Infect Dis, 2008, 14(10):1674-1675.
4. Yang YR, Craig PS, Sun T, et al. Echinococcosis in Ningxia Hui Autonomous Region, northwest China. Trans R Soc Trop Med Hyg, 2008, 102(4):319-328.
5. 张洋, 樊海宁.肝泡型包虫病的治疗进展.临床外科杂志, 2014, 22(7):533-535.
6. Polat P, Kantarci M, Alper F, et al. Hydatid disease from head to toe. Radiographics, 2003, 23(2):475-494.
7. Eckert J, Deplazes P. Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clin Microbiol Rev, 2004, 17(1):107-135.
8. 温浩, 徐明谦.实用包虫病学.北京:科学出版社, 2007:15-19.
9. 王鸿, 李文桂.泡型包虫病免疫发病机制研究进展.地方病通报, 2006, 21(1):98-100.
10. 谢文娟.泡球蚴感染小鼠肝脏基因表达谱的分析.新疆:新疆大学, 2010.
11. Liu YH, Wang XG, Chen YT, et al. Computer tomography of liver in alveolar echinococcosis treated with albendazole. Trans R Soc Trop Med Hyg, 1993, 87(3):319-321.
12. Friedman SL. Hepatic stellate cells:protean, multifunctional, and enigmatic cells of the liver. Physiol Rev, 2008, 88(1):125-172.
13. Geerts A. On the origin of stellate cells:mesodermal, endodermal or neuro-ectodermal. J Hepatol, 2004, 40(2):331-334.
14. 刘昊, 粱廷波.肝星状细胞在肝再生中的作用.中华消化外科杂志, 2011, 10(5):398-400.
15. Brun P, Castagliuolo I, Pinzani M, et al. Exposure to bacterial cell wall products triggers an inflammatory phenotype in hepatic stellate cells. Am J Physiol Gastrointest Liver Physiol, 2005, 289(3):G571-G578.
16. Thirunavukkarasu C, Watkins SC, Gandhi CR. Mechanisms of endotoxin-induced NO, IL-6, and TNF-alpha production in activated rat hepatic stellate cells:role of p38 MAPK. Hepatology, 2006, 44(2):389-398.
17. Czaja MJ, Geerts A, Xu J, et al. Monocyte chemoattractant protein 1(MCP-1) expression occurs in toxic rat liver injury and human liver disease. J Leuk Biol, 1994, 55(1):120-126.
18. 王俊华, 卢晓梅, 张传山, 等.转化生长因子-β1及凋亡相关因子在泡球蚴感染宿主病灶旁免疫细胞中的表达及其意义.中华实验外科杂志, 2011, 28(6):929-931.
19. Hemeda H, Jakob M, Ludwig AK, et al. Interferon-gamma and tumor necrosis factor-alpha differentially affect cytokine expression and migration properties of mesenchymal stem cells. Stem Cells Dev, 2010, 19(5):693-706.
20. Li HY, Ju D, Zhang DW, et al. Activation of TGF-β1-CD147 positive feedback loop in hepatic stellate cells promotes liver fibrosis. Sci Rep, 2015, 5:16552.
21. Yu Y, Lu L, Qian X, et al. Antifibrotic effect of hepatocyte growth factor-expressing mesenchymal stem cells in small-for-size liver transplant rats. Stem Cells Dev, 2010, 19(6):903-914.
22. Parekkadan B, van Poll D, Megeed Z, et al. Immunomodulation of activated hepatic stellate cells by mesenchymal stem cells. Biochem Biophys Res Commun, 2007, 363(2):247-252.
23. Friedman SL. Hepatic fibrosis-overview. Toxicology, 2008, 254(3):120-129.
24. 李婧婷, 汪晖, 廖长秀, 等.肝星状细胞激活的分子机制与抗肝纤维化治疗.武汉大学学报:医学版, 2007, 28(2):256-261.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

泡球蚴囊液对大鼠肝星状细胞TGF-β1、IL-6及TNF-α表达的影响

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