Influence of hepatic artery ligation on hepatocyte apoptosis and liver regeneration of rats with obstructive jaundice_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LIChuan , WANGChuan , ZHANGXiaoyun ,  WENTianfu

Department of Liver Surgery, West China Hospital of Sichuan University, Chengdu 610041, P.R.China;

Corresponding author：

WENTianfu, Email: tianfu1962@163.com

Keywords：

hepatectomy; obstructive jaundice; hepatic artery ligation

DOI：

10.7507/1007-9424.201609056

Video：

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Objective To assess the influence of hepatic artery ligation on survival, hepatocyte apoptosis and regeneration of rats with obstructive jaundice. Methods Eighty adult male Wistar rats were divided into four groups: group A, suffered 70% hepatectomy+hepatic artery ligation+biliary drainage after 3 days of establishing obstructive jaundice model; group B, suffered 70% of hepatectomy+biliary drainage after 3 days of establishing obstructive jaundice model; group C, suffered 70% of hepatectomy+hepatic artery ligation after 3 days of sham operation; group D, suffered 70% of hepatectomy after 3 days of sham operation. Five rats of each group were sacrificed on 1, 2, 3, and 6 days after second operation. Liver function, hepatocyte apoptosis and liver regeneration were detected. Results Postoperative survival rates were not significantly different between group A and group B, similarly between group C and group D (allP＞0.05). There was no significantly different in liver function of group Aversus group B, and group Cversus group D (P＞0.05), but the synthesis of album on 1 d or 3 d after operation were significant difference (group Aversus group B,P＜0.05; group Cversus group D,P＜0.05). Both of the group A, group B and group C had the highest apoptotic index on 1 d after operation, whereas the group D had the lowest hepatic apoptotic index among four group after the surgery. The regeneration indexes were as follow: group D＞group C＞group B＞group A (allP＜0.05). On y 6 d after operation, the regeneration indexes of group A and group B did not increase, while those of group C and group D decreased remarkably. However, the regeneration indexes of four groups were lower than the mean level. Conclusions Hepatic artery ligation will increase hepatocyte apoptosis and weaken liver regeneration. However, for rats with obstructive jaundice, hepatic artery ligation didn't increase the risk of postoperative mortality.

Citation： LIChuan, WANGChuan, ZHANGXiaoyun, WENTianfu. Influence of hepatic artery ligation on hepatocyte apoptosis and liver regeneration of rats with obstructive jaundice. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2017, 24(1): 24-31. doi: 10.7507/1007-9424.201609056 Copy

1.	Aljiffry M, Walsh MJ, Molinari M. Advances in diagnosis, treatment and palliation of cholangiocarcinoma: 1990-2009. World J Gastroenterol, 2009, 15(34): 4240-4262.
2.	Blechacz B, Gores GJ. Cholangiocarcinoma: advances in pathogenesis, diagnosis, and treatment. Hepatology, 2008, 48(1): 308-321.
3.	Chen W, Ke K, Chen YL. Combined portal vein resection in the treatment of hilar cholangiocarcinoma: a systematic review and meta-analysis. Eur J Surg Oncol, 2014, 40(5): 489-495.
4.	de Santibanes E, Ardiles V, Alvarez FA,et al. Hepatic artery reconstruction first for the treatment of hilar cholangiocarcinoma bismuth type IIIB with contralateral arterial invasion: a novel technical strategy. HPB (Oxford), 2012, 14(1): 67-70.
5.	Peng C, Li C, Wen T,et al. Left hepatectomy combined with hepatic artery resection for hilar cholangiocarcinoma: A retrospective cohort study. Int J Surg, 2016, 32: 167-173.
6.	Higgins ES, Banks WL Jr. Cognate effects of ethanol, hydrazine and tissue regeneration on heaptic mitochondrial activities. Biochem Pharmacol, 1971, 20(7): 1513-1524.
7.	Noji T, Tsuchikawa T, Okamura K,et al. Resection and reconstruction of the hepatic artery for advanced perihilar cholangiocarcinoma: result of arterioportal shunting. J Gastrointest Surg, 2015, 19(4): 675-681.
8.	Shimizu H, Hosokawa I, Ohtsuka M,et al. Clinical significance of anatomical variant of the left hepatic artery for perihilar cholangiocarcinoma applied to right-sided hepatectomy. World J Surg, 2014, 38(12): 3210-3214.
9.	Neuhaus P, Jonas S, Bechstein WO,et al. Extended resections for hilar cholangiocarcinoma. Ann Surg, 1999, 230(6): 808-818, discussion 819.
10.	de Jong MC, Marques H, Clary BM,et al. The impact of portal vein resection on outcomes for hilar cholangiocarcinoma: a multi-institutional analysis of 305 cases. Cancer, 2012, 118(19): 4737-4747.
11.	Michels NA. Newer anatomy of the liver and its variant blood supply and collateral circulation. Am J Surg, 1966, 112(3): 337-347.
12.	Michels NA. Collateral arterial pathways to the liver after ligation of the hepatic artery and removal of the celiac axis. Cancer, 1953, 6(4): 708-724.
13.	Michels NA. Newer anatomy of the liver and its variant blood supply and collateral circulation. Am J Surg 1966; 112(3): 337-347.
14.	Beaussier M, Wendum D, Fouassier L,et al. Adaptative bile duct proliferative response in experimental bile duct ischemia. J Hepatol, 2005, 42(2): 257-265.
15.	Faleschini G, Vadala di Prampero SF, Bulajic M,et al. Predictors of endoscopic treatment outcome in the management of biliary complications after orthotopic liver transplantation. Eur J Gastroenterol Hepatol, 2015, 27(2): 150-154.
16.	Goel A, Mehta N, Guy J,et al. Hepatic artery and biliary complications in liver transplant recipients undergoing pretransplant transarterial chemoembolization. Liver Transpl 2014, 20(10): 1221-1228.
17.	Kerr JF, Wyllie AH, Currie AR. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer, 1972, 26(4): 239-257.
18.	Skov Olsen P, Boesby S, Kirkegaard P,et al. Influence of epidermal growth factor on liver regeneration after partial hepatectomy in rats. Hepatology, 1988, 8(5): 992-996.
19.	Margaritis VG, Filos KS, Michalaki MA,et al. Effect of oral glutamine administration on bacterial tanslocation, endotoxemia, liver and ileal morphology, and apoptosis in rats with obstructive jaundice. World J Surg, 2005, 29(10): 1329-1334.
20.	Cindoruk M, Kerem M, Karakan T,et al. Peroxisome proliferators-activated alpha agonist treatment ameliorates hepatic damage in rats with obstructive jaundice: an experimental study. BMC Gastroenterol, 2007, 7: 44.
21.	Sheen-Chen SM, Ho HT, Chia-Pei L,et al. The effect of insulin-like growth factor-I on hepatocyte apoptosis after bile duct ligation in rat. Dig Dis Sci, 2006, 51(12): 2220-2224.
22.	Dirlik M, Karahan A, Canbaz H,et al. Effects of sulfasalazine on lipid peroxidation and histologic liver damage in a rat model of obstructive jaundice and obstructive jaundice with lipopoly-saccharide-induced sepsis. Curr Ther Res Clin Exp, 2009, 70(4): 299-315.
23.	Chen G, Wang S, Bie P,et al. Endogenous bile salts are associated with bile duct injury in the rat liver transplantation model. Transplantation, 2009, 87(3): 330-339.
24.	Joseph B, Kapoor S, Schilsky ML,et al. Bile salt-induced pro-oxidant liver damage promotes transplanted cell proliferation for correcting Wilson disease in the Long-Evans Cinnamon rat model. Hepatology, 2009, 49(5): 1616-1624.

1. Aljiffry M, Walsh MJ, Molinari M. Advances in diagnosis, treatment and palliation of cholangiocarcinoma: 1990-2009. World J Gastroenterol, 2009, 15(34): 4240-4262.
2. Blechacz B, Gores GJ. Cholangiocarcinoma: advances in pathogenesis, diagnosis, and treatment. Hepatology, 2008, 48(1): 308-321.
3. Chen W, Ke K, Chen YL. Combined portal vein resection in the treatment of hilar cholangiocarcinoma: a systematic review and meta-analysis. Eur J Surg Oncol, 2014, 40(5): 489-495.
4. de Santibanes E, Ardiles V, Alvarez FA,et al. Hepatic artery reconstruction first for the treatment of hilar cholangiocarcinoma bismuth type IIIB with contralateral arterial invasion: a novel technical strategy. HPB (Oxford), 2012, 14(1): 67-70.
5. Peng C, Li C, Wen T,et al. Left hepatectomy combined with hepatic artery resection for hilar cholangiocarcinoma: A retrospective cohort study. Int J Surg, 2016, 32: 167-173.
6. Higgins ES, Banks WL Jr. Cognate effects of ethanol, hydrazine and tissue regeneration on heaptic mitochondrial activities. Biochem Pharmacol, 1971, 20(7): 1513-1524.
7. Noji T, Tsuchikawa T, Okamura K,et al. Resection and reconstruction of the hepatic artery for advanced perihilar cholangiocarcinoma: result of arterioportal shunting. J Gastrointest Surg, 2015, 19(4): 675-681.
8. Shimizu H, Hosokawa I, Ohtsuka M,et al. Clinical significance of anatomical variant of the left hepatic artery for perihilar cholangiocarcinoma applied to right-sided hepatectomy. World J Surg, 2014, 38(12): 3210-3214.
9. Neuhaus P, Jonas S, Bechstein WO,et al. Extended resections for hilar cholangiocarcinoma. Ann Surg, 1999, 230(6): 808-818, discussion 819.
10. de Jong MC, Marques H, Clary BM,et al. The impact of portal vein resection on outcomes for hilar cholangiocarcinoma: a multi-institutional analysis of 305 cases. Cancer, 2012, 118(19): 4737-4747.
11. Michels NA. Newer anatomy of the liver and its variant blood supply and collateral circulation. Am J Surg, 1966, 112(3): 337-347.
12. Michels NA. Collateral arterial pathways to the liver after ligation of the hepatic artery and removal of the celiac axis. Cancer, 1953, 6(4): 708-724.
13. Michels NA. Newer anatomy of the liver and its variant blood supply and collateral circulation. Am J Surg 1966; 112(3): 337-347.
14. Beaussier M, Wendum D, Fouassier L,et al. Adaptative bile duct proliferative response in experimental bile duct ischemia. J Hepatol, 2005, 42(2): 257-265.
15. Faleschini G, Vadala di Prampero SF, Bulajic M,et al. Predictors of endoscopic treatment outcome in the management of biliary complications after orthotopic liver transplantation. Eur J Gastroenterol Hepatol, 2015, 27(2): 150-154.
16. Goel A, Mehta N, Guy J,et al. Hepatic artery and biliary complications in liver transplant recipients undergoing pretransplant transarterial chemoembolization. Liver Transpl 2014, 20(10): 1221-1228.
17. Kerr JF, Wyllie AH, Currie AR. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer, 1972, 26(4): 239-257.
18. Skov Olsen P, Boesby S, Kirkegaard P,et al. Influence of epidermal growth factor on liver regeneration after partial hepatectomy in rats. Hepatology, 1988, 8(5): 992-996.
19. Margaritis VG, Filos KS, Michalaki MA,et al. Effect of oral glutamine administration on bacterial tanslocation, endotoxemia, liver and ileal morphology, and apoptosis in rats with obstructive jaundice. World J Surg, 2005, 29(10): 1329-1334.
20. Cindoruk M, Kerem M, Karakan T,et al. Peroxisome proliferators-activated alpha agonist treatment ameliorates hepatic damage in rats with obstructive jaundice: an experimental study. BMC Gastroenterol, 2007, 7: 44.
21. Sheen-Chen SM, Ho HT, Chia-Pei L,et al. The effect of insulin-like growth factor-I on hepatocyte apoptosis after bile duct ligation in rat. Dig Dis Sci, 2006, 51(12): 2220-2224.
22. Dirlik M, Karahan A, Canbaz H,et al. Effects of sulfasalazine on lipid peroxidation and histologic liver damage in a rat model of obstructive jaundice and obstructive jaundice with lipopoly-saccharide-induced sepsis. Curr Ther Res Clin Exp, 2009, 70(4): 299-315.
23. Chen G, Wang S, Bie P,et al. Endogenous bile salts are associated with bile duct injury in the rat liver transplantation model. Transplantation, 2009, 87(3): 330-339.
24. Joseph B, Kapoor S, Schilsky ML,et al. Bile salt-induced pro-oxidant liver damage promotes transplanted cell proliferation for correcting Wilson disease in the Long-Evans Cinnamon rat model. Hepatology, 2009, 49(5): 1616-1624.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Influence of hepatic artery ligation on hepatocyte apoptosis and liver regeneration of rats with obstructive jaundice

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