Objective  To investigate the proportions of CD4⁺ T cells, CD8⁺ T cells, and mutant of p53 gene in the microenvironment of breast infiltrating ductal carcinoma, and to explore its’ correlation with prognosis of breast infiltrating ductal carcinoma. Methods  Eighty-five cases of breast infiltrating ductal carcinoma were collected who underwent surgery in the 371^st Central Hospital of Peoples’ Liberation Army from 2010 to 2012, and then detected the proportion of CD4⁺ T cells and CD8⁺ T cells, ratio of CD4⁺ T cells to CD8⁺ T cells, and mutant of p53 gene in the cancer tissues with immunohistochemistry. Comparison between the sentinel lymph node metastasis group and non-sentinel lymph node metastasis group, mutant of p53 gene group and non-mutant of p53 gene group on the proportions of CD4⁺ T cells, CD8⁺ T cells, and ratio of CD4⁺ T cells to CD8⁺ T cells were performed, as well as the relationship between proportion of CD8⁺ T cells/mutant of p53 gene and prognosis of breast infiltrating ductal carcinoma. Results  ① The relationship between proportion of CD4⁺ T cells/proportion of CD8⁺ T cells/ratio of CD4⁺ T cells to CD8⁺ T cells and situation of sentinel lymph node metastasis: at cluster, compared with the sentinel lymph node metastasis group, the proportion of CD8⁺ T cells was lower in the non-sentinel lymph node metastasis group (P<0.05), but there was no significant difference on the proportion of CD4⁺ T cells and ratio of CD4⁺ T cells to CD8⁺ T cells (P>0.05); at stroma, compared with the sentinel lymph node metastasis group, the proportions of CD4⁺ T cells and CD8⁺ T cells were lower, but the ratio of CD4⁺ T cells to CD8⁺ T cells was higher in the non-sentinel lymph node metastasis group (P<0.05). ② The relationship between proportion of CD4⁺ T cells/proportion of CD8⁺ T cells/ratio of CD4⁺ T cells to CD8⁺ T cells and mutant of p53 gene: both at the cluster and stroma, compared with the mutant of p53 gene group, the proportions of CD4⁺ T cells and CD8⁺ T cells were lower, but the ratio of CD4⁺ T cells to CD8⁺ T cells was higher in the non-mutant of p53 gene group (P<0.05). ③ The relationship between proportion of CD8⁺ T cells/mutant of p53 gene and prognosis of breast infiltrating ductal carcinoma: the prognosis was worse in patients with high degree of infiltration of CD8⁺ T cells and mutant of p53 gene than those patients with low degree of infiltration of CD8⁺ T cells and non-mutant of p53 gene (P<0.05). Conclusions  The proportions of CD4⁺ T cells and CD8⁺ T cells, and ratio of CD4⁺ T cells to CD8⁺ T cells are associated with the situation of sentinel lymph node metastasis and mutant of p53 gene, and the degree of infiltration of CD8⁺ T cells and mutant of p53 gene are associated with the prognosis of breast infiltrating ductal carcinoma.

Citation： XU Jingjing, LIU Feng, YANG Tingtong, LU Jianfu. The proportion of CD4⁺ T cells, CD8⁺ T cells, and mutant of p53 gene in the micro- environment of breast infiltrating ductal carcinoma . CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2018, 25(3): 328-333. doi: 10.7507/1007-9424.201709032 Copy