SONG Lipo 1 , ZHANG Jian 1 , GU Yongquan 1 , CHEN Bing 2 , GUO Lianrui 1 , WANG Chunmei 1 , WU Yingfeng 1 , LUO Tao 1 , CUI Shijun 1 , WANG Zhonggao 1
  • 1. Department of Vascular Surgery, Xuanwu Hospital, Institute of Vascular Surgery, Capital Medical University, Beijing 100053, P. R. China;
  • 2. Department of Vascular Surgery, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, P. R. China;
GU Yongquan, Email: gu-yq@263.net
neovacularization; hypoxia; bone morrow-derived stem cell; endothelial progenitor cell; critical limb ischemia
10.7507/1007-9424.201710088
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Objective  To evaluate effect of hypoxia condition (1% or 5% oxygen concentration) on proliferation, adhesion, migration, or viability ability of bone morrow-derived endothelial progenitor cells (EPCs). Methods  The bone marrow mononuclear cells of SD rat were acquired with density gradient centrifugation method. They were cultured, induced, and differentiated to the EPCs. Then they were cultured respectively in three different oxygen concentrations (1%, 5%, or 21%). On the 3rd day and the 7th day, the effects of the different oxygen concentrations (1%, 5%, or 21%) on the EPCs’ neovascularization characteristics (including proliferation, adhesion, migration, and viability abilities) were evaluated. Results  Whether cultured for the 3rd day or 7th day, the proliferation, adhesion, migration, and viability abilities of the cultured cells in the 1% and 5% oxygen concentrations were significantly better than those of the cultured cells in the 21% oxygen concentration (all P<0.05). Except for the proliferation ability of the cultured cells in the 5% oxygen concentration was significantly better than that of the cultured cells in the 1% oxygen concentration (P<0.05) on the 3rd day, and the adhesion ability on the 3rd day and the proliferation ability on the 7th day had no significantly differences, the other abilities (adhesion, migration, and viability abilities) of the cultured cells in the 1% oxygen concentration were significantly better than those of the cultured cells in the 5% oxygen concentration (allP<0.05). Conclusion  Different oxygen concentration has an effect on proliferation, adhesion, migration, or viability ability of bone morrow-derived EPCs, appropriate hypoxia condition (1% or 5% oxygen concentration ) can enhance these abilities.

Citation： SONG Lipo, ZHANG Jian, GU Yongquan, CHEN Bing, GUO Lianrui, WANG Chunmei, WU Yingfeng, LUO Tao, CUI Shijun, WANG Zhonggao. Hypoxia condition can enhance proliferation, adhesion, migration, and viability abilities of bone morrow-derived endothelial progenitor cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2018, 25(5): 528-533. doi: 10.7507/1007-9424.201710088 Copy

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