LIN Yanyan 1 , ZHANG Zhoujing 2 , CAO Jie 3 , BAI Bing 1 , YUE Ping 1 , BA Yongjiang 4 , MENG Wenbo 1
  • 1. Department of Special Minimally Invasive Surgery, The First Hospital of Lanzhou University, Lanzhou 730000, P. R. China;
  • 2. Department of Thyroid Surgery, Ningbo No.2 Hospital, Ningbo, Zhejiang 315000, P. R. China;
  • 3. Clinical Laboratory, The First Hospital of Lanzhou University, Lanzhou 730000, P. R. China;
  • 4. The First Clinical College of Lanzhou University, Lanzhou 730000, P. R. China;
MENG Wenbo, Email: mengwb@lzu.edu.cn
miRNA; marker; hepatocellular carcinoma; miR-483-5p; alpha fetoprotein
10.7507/1007-9424.201711051
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Objective  To detect expression of miR-483-5p in surem of patients with hepatocellular carcinoma (HCC) and investigate it’s clinical significance for diagnosis of HCC. Methods  The rerum samples of 112 patients with HCC (HCC group), 85 patients with chronic viral hepatitis B (CHB group), and 56 healthy people for physical examination (healthy control group) were collected from January 2010 to January 2012 in the First Hospital of Lanzhou University. According to the results of preliminary chip detection of miRCURY LNATM miRNA, the real-time fluorescent quantitative PCR was adopted to quantitate the serum levels of miR-483-5p and miR-500a and the routine electrochemical method was used to detect the serum alpha fetoprotein (AFP) in every group. The receiver operating characteristic (ROC) curve was utilized to analyze the diagnostic values of serum miR-483-5p, miR-500a, and AFP for the HCC. Results  The serum levels of miR-483-5p and miR-500a in the HCC group were significantly higher than those of the CHB and healthy control groups (both P<0.000 1), which had no significant differences between the CHB group and the healthy control group (P>0.05). The serum miR-483-5p level of the HCC patient decreased markedly at the postoperative 30 d (P<0.000 1) as compared with the preoperative level. The area under the ROC curve (AUC) of miR-483-5p, miR-500a, AFP, or miR-483-5p in combination with AFP for the diagnosis of the HCC was 0.74 (cutoff value=2.842, sensitivity=74% and specificity=66%), 0.66 (cutoff value=1.830, sensitivity=74% and specificity=51%), 0.81 (cutoff value=20 μg/L, sensitivity=78% and specificity=70%), and 0.92 (cutoff value=3.78, sensitivity=81% and specificity=83%), respectively. The AUC values of miR-483-5p in the diagnosis of the HCC patients with positive AFP (AFP>20 μg/L) and negative AFP (0–20 μg/L) were 0.78 and 0.83, respectively. Conclusions  Serum miR-483-5p highly expresses in HCC, which has a certain accuracy in diagnosis of HCC, it combined with AFP could further increase its diagnostic value. Serum miR-483-5p might play an important supplemental role in diagnosis of HCC patient with negative AFP.

Citation： LIN Yanyan, ZHANG Zhoujing, CAO Jie, BAI Bing, YUE Ping, BA Yongjiang, MENG Wenbo. Expresssion of miR-483-5p in serum of patients with hepatocellular carcinoma and it’s clinical significance. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2018, 25(6): 689-694. doi: 10.7507/1007-9424.201711051 Copy

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