Expression of long non-coding RNA GAS5 in hepatocellular carcinoma tissues and its clinical significance_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

QIU Jianguo , SHI Zhengrong ,  LI Ming

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P. R. China;

Corresponding author：

LI Ming, Email: limingcyfyy945@163.com

Keywords：

hepatocellular carcinoma; growth arrest-specific 5; long non-coding RNA; prognosis

DOI：

10.7507/1007-9424.201807021

Video：

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Objective To investigate the expression of growth arrest-specific 5 (GAS5) mRNA and its clinical significance in hepatocellular carcinoma.Methods The expression of GAS5 mRNA in the hepatocellular carcinoma tissues and corresponding adjacent tissues were detected by real time-PCR. The relationship between the expression of GAS5 mRNA and clinicopathological characteristics were analyzed by SPSS 19.0 software.Results The expression of GAS5 mRNA in hepatocellular carcinoma tissues was significantly lower than that of the adjacent tissues (P<0.01). The expression of GAS5 mRNA was related to tumor size, tumor number, lymph node metastasis, clinical TNM stage, alpha fetoprotein level, and tumor differentiation (P<0.05). Cox hazard model results showed that low expression of GAS5 mRNA was associated with poor prognosis (P<0.05).Conclusion GAS5 mRNA is expected to be a diagnostic and prognostic marker for patients with hepatocellular carcinoma.

Citation： QIU Jianguo, SHI Zhengrong, LI Ming. Expression of long non-coding RNA GAS5 in hepatocellular carcinoma tissues and its clinical significance. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2019, 26(3): 301-306. doi: 10.7507/1007-9424.201807021 Copy

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2.	Chang CH, Chau GY, Lui WY, et al. Long-term results of hepatic resection for hepatocellular carcinoma originating from the noncirrhotic liver. Arch Surg, 2004, 139(3): 320-325.
3.	Gupta RA, Shah N, Wang KC, et al. Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Nature, 2010, 464(7291): 1071-1076.
4.	Liu XH, Sun M, Nie FQ, et al. Lnc RNA HOTAIR functions as a competing endogenous RNA to regulate HER2 expression by sponging miR-331-3p in gastric cancer. Mol Cancer, 2014, 13: 92.
5.	Li L, Feng T, Lian Y, et al. Role of human noncoding RNAs in the control of tumorigenesis. Proc Natl Acad Sci U S A, 2009, 106(31): 12956-12961.
6.	Qiao HP, Gao WS, Huo JX, et al. Long non-coding RNA GAS5 functions as a tumor suppressor in renal cell carcinoma. Asian Pac J Cancer Prev, 2013, 14(2): 1077-1082.
7.	Zhang Z, Zhu Z, Watabe K, et al. Negative regulation of lncRNA GAS5 by miR-21. Cell Death Differ, 2013, 20(11): 1558-1568.
8.	Pickard MR, Williams GT. Regulation of apoptosis by long non-coding RNA GAS5 in breast cancer cells: implications for chemotherapy. Breast Cancer Res Treat, 2014, 145(2): 359-370.
9.	Pickard MR, Mourtada-Maarabouni M, Williams GT. Long non-coding RNA GAS5 regulates apoptosis in prostate cancer cell lines. Biochim Biophys Acta, 2013, 1832(10): 1613-1623.
10.	Shi X, Sun M, Liu H, et al. A critical role for the long non-coding RNA GAS5 in proliferation and apoptosis in non-small-cell lung cancer. Mol Carcinog, 2015, 54(Suppl 1): E1-E12.
11.	Cao Q, Wang N, Qi J, et al. Long non-coding RNA-GAS5 acts as a tumor suppressor in bladder transitional cell carcinoma via regulation of chemokine (C-C motif) ligand 1 expression. Mol Med Rep, 2016, 13(1): 27-34.
12.	Tu ZQ, Li RJ, Mei JZ, et al. Down-regulation of long non-coding RNA GAS5 is associated with the prognosis of hepatocellular carcinoma. Int J Clin Exp Pathol, 2014, 7(7): 4303-4309.
13.	景伟, 罗萍, 朱满, 等. 血浆长链非编码 RNA GAS5在肝细胞癌中表达水平及治疗的评估. 中华检验医学杂志, 2016, 39(8): 599-603.
14.	Edge SB, Byrd DR, Green FL, et al. American Joint Committee on Cancer Staging Manual. 7^th edition. New York: Springer, 2010: 242-251.
15.	Adesina AM, Rauch RA. Ganglioglioma and ganglio- cytoma//Adesina AM, Tihan T, Fuller CE, et al. Atlas of Pediatric Brian Tumors. Springer, 2010: 181-191.
16.	姚凯, 朱光辉, 单远洲. 长链非编码 RNA TUG1 和 UCA1 在结肠癌组织中的表达及临床意义. 中国普通外科杂志, 2018, 27(4): 449-456.
17.	苗润晨, 许鑫森, 陈伟, 等. 年龄因素对肝癌肝切除术患者长期预后的影响. 现代肿瘤医学, 2014, 22(5): 1123-1126.
18.	高纪东, 邵永孚, 吴健雄, 等. 肝细胞癌肝切除术后预后影响因素分析. 中华肝胆外科杂志, 2005, 11(8): 515-517.
19.	蒋天安, 陈立斌, 陈芬, 等. 肝恶性肿瘤超声引导下射频消融短期有效性及影响因素分析. 中华超声影像学杂志, 2013, 22(1): 25-29.
20.	石明, 张颖, 钟崇, 等. 肝癌患者围手术期外周血甲胎蛋白 mRNA 表达与术后复发的关系. 癌症, 2008, 27(1): 83-87.
21.	吕文平, 董家鸿, 张文智, 等. 外科医师手术操作对肝细胞癌预后的影响因素. 中国医学科学院学报, 2008, 30(4): 386-392.
22.	梁冠林, 陈哲宇, 文天夫, 等. 常温下连续阻断半肝血流 60 min 在乙肝肝硬变患者肝切除术中的应用. 中国普外基础与临床杂志, 2008, 15(7): 503-507.
23.	刘景丰, 池闽辉, 曾金华, 等. 肝血流阻断方法对肝切除术中大出血的影响. 中华外科杂志, 2010, 48(3): 177-180.
24.	Schones DE, Zhao K. Genome-wide approaches to studying chromatin modifications. Nat Rev Genet, 2008, 9(3): 179-191.
25.	孙甜甜, 房静远. 长非编码 RNA 在肝癌中的研究进展. 肿瘤, 2011, 31(9): 867-870.
26.	Gabory A, Ripoche MA, Yoshimizu T, et al. The H19 gene: regulation and function of a non-coding RNA. Cytogenet Genome Res, 2006, 113(1-4): 188-193.
27.	Gutschner T, Hämmerle M, Eissmann M, et al. The noncoding RNA MALAT1 is a critical regulator of the metastasis phenotype of lung cancer cells. Cancer Res, 2013, 73(3): 1180-1189.
28.	Li H, An J, Wu M, et al. LncRNA HOTAIR promotes human liver cancer stem cell malignant growth through downregulation of SETD2. Oncotarget, 2015, 6(29): 27847-27864.
29.	Braconi C, Valeri N, Kogure T, et al. Expression and functional role of a transcribed noncoding RNA with an ultraconserved element in hepatocellular carcinoma. Proc Natl Acad Sci U S A, 2011, 108(2): 786-791.
30.	Braconi C, Kogure T, Valeri N, et al. microRNA-29 can regulate expression of the long non-coding RNA gene MEG3 in hepatocellular cancer. Oncogene, 2011, 30(47): 4750-4756.
31.	Lu X, Fang Y, Wang Z, et al. Downregulation of gas5 increases pancreatic cancer cell proliferation by regulating CDK6. Cell Tissue Res, 2013, 354(3): 891-896.

1. Altekruse SF, McGlynn KA, Reichman ME. Hepatocellular carcinoma incidence, mortality, and survival trends in the United States from 1975 to 2005. J Clin Oncol, 2009, 27(9): 1485-1491.
2. Chang CH, Chau GY, Lui WY, et al. Long-term results of hepatic resection for hepatocellular carcinoma originating from the noncirrhotic liver. Arch Surg, 2004, 139(3): 320-325.
3. Gupta RA, Shah N, Wang KC, et al. Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Nature, 2010, 464(7291): 1071-1076.
4. Liu XH, Sun M, Nie FQ, et al. Lnc RNA HOTAIR functions as a competing endogenous RNA to regulate HER2 expression by sponging miR-331-3p in gastric cancer. Mol Cancer, 2014, 13: 92.
5. Li L, Feng T, Lian Y, et al. Role of human noncoding RNAs in the control of tumorigenesis. Proc Natl Acad Sci U S A, 2009, 106(31): 12956-12961.
6. Qiao HP, Gao WS, Huo JX, et al. Long non-coding RNA GAS5 functions as a tumor suppressor in renal cell carcinoma. Asian Pac J Cancer Prev, 2013, 14(2): 1077-1082.
7. Zhang Z, Zhu Z, Watabe K, et al. Negative regulation of lncRNA GAS5 by miR-21. Cell Death Differ, 2013, 20(11): 1558-1568.
8. Pickard MR, Williams GT. Regulation of apoptosis by long non-coding RNA GAS5 in breast cancer cells: implications for chemotherapy. Breast Cancer Res Treat, 2014, 145(2): 359-370.
9. Pickard MR, Mourtada-Maarabouni M, Williams GT. Long non-coding RNA GAS5 regulates apoptosis in prostate cancer cell lines. Biochim Biophys Acta, 2013, 1832(10): 1613-1623.
10. Shi X, Sun M, Liu H, et al. A critical role for the long non-coding RNA GAS5 in proliferation and apoptosis in non-small-cell lung cancer. Mol Carcinog, 2015, 54(Suppl 1): E1-E12.
11. Cao Q, Wang N, Qi J, et al. Long non-coding RNA-GAS5 acts as a tumor suppressor in bladder transitional cell carcinoma via regulation of chemokine (C-C motif) ligand 1 expression. Mol Med Rep, 2016, 13(1): 27-34.
12. Tu ZQ, Li RJ, Mei JZ, et al. Down-regulation of long non-coding RNA GAS5 is associated with the prognosis of hepatocellular carcinoma. Int J Clin Exp Pathol, 2014, 7(7): 4303-4309.
13. 景伟, 罗萍, 朱满, 等. 血浆长链非编码 RNA GAS5在肝细胞癌中表达水平及治疗的评估. 中华检验医学杂志, 2016, 39(8): 599-603.
14. Edge SB, Byrd DR, Green FL, et al. American Joint Committee on Cancer Staging Manual. 7^th edition. New York: Springer, 2010: 242-251.
15. Adesina AM, Rauch RA. Ganglioglioma and ganglio- cytoma//Adesina AM, Tihan T, Fuller CE, et al. Atlas of Pediatric Brian Tumors. Springer, 2010: 181-191.
16. 姚凯, 朱光辉, 单远洲. 长链非编码 RNA TUG1 和 UCA1 在结肠癌组织中的表达及临床意义. 中国普通外科杂志, 2018, 27(4): 449-456.
17. 苗润晨, 许鑫森, 陈伟, 等. 年龄因素对肝癌肝切除术患者长期预后的影响. 现代肿瘤医学, 2014, 22(5): 1123-1126.
18. 高纪东, 邵永孚, 吴健雄, 等. 肝细胞癌肝切除术后预后影响因素分析. 中华肝胆外科杂志, 2005, 11(8): 515-517.
19. 蒋天安, 陈立斌, 陈芬, 等. 肝恶性肿瘤超声引导下射频消融短期有效性及影响因素分析. 中华超声影像学杂志, 2013, 22(1): 25-29.
20. 石明, 张颖, 钟崇, 等. 肝癌患者围手术期外周血甲胎蛋白 mRNA 表达与术后复发的关系. 癌症, 2008, 27(1): 83-87.
21. 吕文平, 董家鸿, 张文智, 等. 外科医师手术操作对肝细胞癌预后的影响因素. 中国医学科学院学报, 2008, 30(4): 386-392.
22. 梁冠林, 陈哲宇, 文天夫, 等. 常温下连续阻断半肝血流 60 min 在乙肝肝硬变患者肝切除术中的应用. 中国普外基础与临床杂志, 2008, 15(7): 503-507.
23. 刘景丰, 池闽辉, 曾金华, 等. 肝血流阻断方法对肝切除术中大出血的影响. 中华外科杂志, 2010, 48(3): 177-180.
24. Schones DE, Zhao K. Genome-wide approaches to studying chromatin modifications. Nat Rev Genet, 2008, 9(3): 179-191.
25. 孙甜甜, 房静远. 长非编码 RNA 在肝癌中的研究进展. 肿瘤, 2011, 31(9): 867-870.
26. Gabory A, Ripoche MA, Yoshimizu T, et al. The H19 gene: regulation and function of a non-coding RNA. Cytogenet Genome Res, 2006, 113(1-4): 188-193.
27. Gutschner T, Hämmerle M, Eissmann M, et al. The noncoding RNA MALAT1 is a critical regulator of the metastasis phenotype of lung cancer cells. Cancer Res, 2013, 73(3): 1180-1189.
28. Li H, An J, Wu M, et al. LncRNA HOTAIR promotes human liver cancer stem cell malignant growth through downregulation of SETD2. Oncotarget, 2015, 6(29): 27847-27864.
29. Braconi C, Valeri N, Kogure T, et al. Expression and functional role of a transcribed noncoding RNA with an ultraconserved element in hepatocellular carcinoma. Proc Natl Acad Sci U S A, 2011, 108(2): 786-791.
30. Braconi C, Kogure T, Valeri N, et al. microRNA-29 can regulate expression of the long non-coding RNA gene MEG3 in hepatocellular cancer. Oncogene, 2011, 30(47): 4750-4756.
31. Lu X, Fang Y, Wang Z, et al. Downregulation of gas5 increases pancreatic cancer cell proliferation by regulating CDK6. Cell Tissue Res, 2013, 354(3): 891-896.

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Expression of long non-coding RNA GAS5 in hepatocellular carcinoma tissues and its clinical significance

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