The expressions and clinical significance of NF-κBp65 and autophagy related proteins Beclin1 and p62 in thyroid papillary carcinoma_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LU Bin , ZHANG Peng , ZHANG Yantao ,  ZHANG Qiwen

Department of Thyroid and Breast Surgery, Jinan People's Hospital, Jinan 271100, P. R. China;

Corresponding author：

ZHANG Qiwen, Email: lwzqw@126.com

Keywords：

thyroid cancer; lymph node metastasis; NF-κBp65; autophagy; Beclin1 protein; p62 protein

DOI：

10.7507/1007-9424.201906051

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To analyze the expression and significance of NF-κBp65 and autophagy-related proteins Beclin1 and p62 in patients with papillary thyroid carcinoma (PTC).Methods One hundred and sixty cases of PTC patients' tumor tissue specimens and paracancerous tissue specimens in our hospital from March 2013 to February 2015 were collected, and 90 cases of cervical lymph node metastasis tissue specimens of the above patients were collected. The expressions of NF-κBp65, Beclin1 and p62 in PTC tissues, metastatic lymph node tissues and paracancerous tissues were detected by immunohistochemical method, and the relationship between the above indexes and the clinicopathological characteristics and prognosis of PTC patients was analyzed.Results The positive rates of expression of NF-kappa Bp65 and p62 in PTC tissues and metastatic lymph node tissues were higher than those in paracancerous tissues (P<0.05). The expression rate of Beclin1 in PTC tissues and metastatic lymph node tissues was lower than that in paracancerous tissues (P<0.05). The positive rate of NF-κBp65 expression in PTC tissues was not related to the clinicopathological characteristics of patients (P>0.05). The expression of p62 decreased with the increase of tumor differentiation (P<0.05). The expression of Beclin1 in patients with stage Ⅲ+Ⅳ and lymph node metastasis were lower than those in patients with stage Ⅰ+Ⅱ and without lymph node metastasis (P<0.05), while the expression of p62 was opposite. Spearman correlation analysis showed that the expression of Beclin1 and p62 in PTC tissues was negatively correlated (r=–0.656, P<0.01). In metastatic lymph node tissues, the expression of Beclin1 and p62 was also negatively correlated (r=–0.562, P<0.01). The 3-year survival rates of patients with positive expression of p62 and NF-κBp65 in PTC tissues were lower than that of patients with negative expression (P<0.05). The 3-year survival rate of patients with positive expression of Becrin1 was higher than that of negative expression (P<0.05). TNM stage, lymph node metastasis, NF-κBp65 and p62 were independent risk factors for PTC prognosis, and Beclin1 was protective factor.Conclusions NF-κBp65 and p62 are highly expressed in PTC tissues and lymph node metastasis tissues, while Beclin1 is poorly expressed, which could be used as independent prognostic factors for PTC patients. In addition, Beclin1 and p62 are related to PTC biological behavior and may become potential indicators for PTC diagnosis.

Citation： LU Bin, ZHANG Peng, ZHANG Yantao, ZHANG Qiwen. The expressions and clinical significance of NF-κBp65 and autophagy related proteins Beclin1 and p62 in thyroid papillary carcinoma. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2019, 26(11): 1304-1310. doi: 10.7507/1007-9424.201906051 Copy

1.	刘文, 程若川. 甲状腺乳头状癌中央区淋巴结转移的预测因素. 中国普外基础与临床杂志, 2018, 25(1): 99-103.
2.	范晓峰, 李晓东, 周冬. 低位弧形切口法与" L”型切口法在甲状腺癌合并颈部淋巴结转移患者手术中的应用. 医学临床研究, 2016, 33(5): 916-918.
3.	Vegliante R, Desideri E, Di Leo L, et al. Dehydroepiandrosterone triggers autophagic cell death in human hepatoma cell line HepG2 via JNK-mediated p62/SQSTM1 expression. Carcinogenesis, 2016, 37(3): 233-244.
4.	刘美快, 袁哲英, 黄凯西, 等. 低强度超声联合微泡通过提高 ROS 水平促进甲状腺癌细胞自噬性死亡. 中国病理生理杂志, 2017, 33(11): 2000-2008.
5.	Taniguchi K, Karin M. NF-κB, inflammation, immunity and cancer: coming of age. Nat Rev Immunol, 2018, 18(5): 309-324.
6.	Liu B, Sun L, Liu Q, et al. A cytoplasmic NF-κB interacting long noncoding RNA blocks IκB phosphorylation and suppresses breast cancer metastasis. Cancer cell, 2015, 27(3): 370-381.
7.	郑向前, 林琳, 夏婷婷, 等. 美国甲状腺学会 2009 年分化型甲状腺癌诊治指南解读. 中华普通外科杂志, 2011, 26(4): 349-352.
8.	王晓蔚, 康凯夫. Beclin1 和 NF-κBp65 表达与肝癌发生发展的相关性研究. 中华肿瘤防治杂志, 2009, 16(13): 974-977.
9.	李田军, 林岩松, 梁军, 等. 分化型甲状腺癌相关基因的研究进展. 中华肿瘤防治杂志, 2011, 18(11): 896-900.
10.	Ahn S, Kim TH, Kim SW, et al. Comprehensive screening for PD-L1 expression in thyroid cancer. Endocr-Relat Cancer, 2017, 24(2): 97-106.
11.	TKim EJ, Jeong JH, Bae S, et al. mTOR inhibitors radiosensitize PTEN-deficient non-small-cell lung cancer cells harboring an EGFR activating mutation by inducing autophagy. J Cell Biochem, 2013, 114(6): 1248-1256.
12.	韩颖, 吴正升, 吴强. 乳腺癌新辅助化疗前后 p62 和XIAP表达的变化及其意义. 临床与实验病理学杂志, 2017, 33(5): 481-484.
13.	Dong S, Qu X, Li W, et al. The long non-coding RNA, GAS5, enhances gefitinib-induced cell death in innate EGFR tyrosine kinase inhibitor-resistant lung adenocarcinoma cells with wide-type EGFR via downregulation of the IGF-1R expression. J Hematol Oncol, 2015, 8(1): 43.
14.	Rohatgi RA, Janusis J, Leonard D, et al. Beclin 1 regulates growth factor receptor signaling in breast cancer. Oncogene, 2015, 34(42): 5352-5362.
15.	Chang MA, Morgado M, Warren CR, et al. p62/SQSTM1 is required for cell survival of apoptosis-resistant bone metastatic prostate cancer cell lines. Prostate, 2014, 74(2): 149-163.
16.	谢昆, 李密杰, 蒋成砚, 等. 自噬相关蛋白 ATG5/BECLIN-1 调控细胞自噬和凋亡的分子机理研究进展. 中国人兽共患病学报, 2018, 34(3): 272-275.
17.	侯德法. 胃癌组织中 LC3B 和 p62 的表达和临床意义. 安徽医药, 2017, 21(9): 1647-1650.
18.	Yu T, Guo F, Yu Y, et al. Fusobacterium nucleatum promotes chemoresistance to colorectal cancer by modulating autophagy. Cell, 2017, 170(3): 548-563.
19.	王幼辉, 王锡恩, 彭敏, 等. 自噬相关蛋白 Beclin1、p62、Atg5 在浆液性卵巢癌组织中的表达及临床意义. 中华内分泌外科杂志, 2016, 10(4): 329-332.
20.	Camargo R, Riccardi D, Ribeiro H, et al. NF-κBp65 and expression of its pro-inflammatory target genes are upregulated in the subcutaneous adipose tissue of cachectic cancer patients. Nutrients, 2015, 7(6): 4465-4479.
21.	Kang K F, Wang X W, Chen X W, et al. Beclin 1 and nuclear factor-κBp65 are upregulated in hepatocellular carcinoma. Oncology Letters, 2013, 5(6): 1813-1818.
22.	廖桂周. NIBP 和 NF-κBp65 在胃癌中的表达及其临床意义. 中国医药科学, 2016, 6(14): 27-30.
23.	Guo YZ, He P, Feng AM. Effect of curcumin on expressions of NF-κ Bp65,TNF-α and IL-8 in placental tissue of premature birth of infected mice. Asian Pac J Trop Med, 2017, 10(2): 168-171.
24.	Zhu J, Yang L, Chen C, et al. NF-κB p65 overexpression promotes bladder cancer cell migration via FBW7-mediated degradation of RhoGDIα protein. Neoplasia, 2017, 19(9): 672-683.

1. 刘文, 程若川. 甲状腺乳头状癌中央区淋巴结转移的预测因素. 中国普外基础与临床杂志, 2018, 25(1): 99-103.
2. 范晓峰, 李晓东, 周冬. 低位弧形切口法与" L”型切口法在甲状腺癌合并颈部淋巴结转移患者手术中的应用. 医学临床研究, 2016, 33(5): 916-918.
3. Vegliante R, Desideri E, Di Leo L, et al. Dehydroepiandrosterone triggers autophagic cell death in human hepatoma cell line HepG2 via JNK-mediated p62/SQSTM1 expression. Carcinogenesis, 2016, 37(3): 233-244.
4. 刘美快, 袁哲英, 黄凯西, 等. 低强度超声联合微泡通过提高 ROS 水平促进甲状腺癌细胞自噬性死亡. 中国病理生理杂志, 2017, 33(11): 2000-2008.
5. Taniguchi K, Karin M. NF-κB, inflammation, immunity and cancer: coming of age. Nat Rev Immunol, 2018, 18(5): 309-324.
6. Liu B, Sun L, Liu Q, et al. A cytoplasmic NF-κB interacting long noncoding RNA blocks IκB phosphorylation and suppresses breast cancer metastasis. Cancer cell, 2015, 27(3): 370-381.
7. 郑向前, 林琳, 夏婷婷, 等. 美国甲状腺学会 2009 年分化型甲状腺癌诊治指南解读. 中华普通外科杂志, 2011, 26(4): 349-352.
8. 王晓蔚, 康凯夫. Beclin1 和 NF-κBp65 表达与肝癌发生发展的相关性研究. 中华肿瘤防治杂志, 2009, 16(13): 974-977.
9. 李田军, 林岩松, 梁军, 等. 分化型甲状腺癌相关基因的研究进展. 中华肿瘤防治杂志, 2011, 18(11): 896-900.
10. Ahn S, Kim TH, Kim SW, et al. Comprehensive screening for PD-L1 expression in thyroid cancer. Endocr-Relat Cancer, 2017, 24(2): 97-106.
11. TKim EJ, Jeong JH, Bae S, et al. mTOR inhibitors radiosensitize PTEN-deficient non-small-cell lung cancer cells harboring an EGFR activating mutation by inducing autophagy. J Cell Biochem, 2013, 114(6): 1248-1256.
12. 韩颖, 吴正升, 吴强. 乳腺癌新辅助化疗前后 p62 和XIAP表达的变化及其意义. 临床与实验病理学杂志, 2017, 33(5): 481-484.
13. Dong S, Qu X, Li W, et al. The long non-coding RNA, GAS5, enhances gefitinib-induced cell death in innate EGFR tyrosine kinase inhibitor-resistant lung adenocarcinoma cells with wide-type EGFR via downregulation of the IGF-1R expression. J Hematol Oncol, 2015, 8(1): 43.
14. Rohatgi RA, Janusis J, Leonard D, et al. Beclin 1 regulates growth factor receptor signaling in breast cancer. Oncogene, 2015, 34(42): 5352-5362.
15. Chang MA, Morgado M, Warren CR, et al. p62/SQSTM1 is required for cell survival of apoptosis-resistant bone metastatic prostate cancer cell lines. Prostate, 2014, 74(2): 149-163.
16. 谢昆, 李密杰, 蒋成砚, 等. 自噬相关蛋白 ATG5/BECLIN-1 调控细胞自噬和凋亡的分子机理研究进展. 中国人兽共患病学报, 2018, 34(3): 272-275.
17. 侯德法. 胃癌组织中 LC3B 和 p62 的表达和临床意义. 安徽医药, 2017, 21(9): 1647-1650.
18. Yu T, Guo F, Yu Y, et al. Fusobacterium nucleatum promotes chemoresistance to colorectal cancer by modulating autophagy. Cell, 2017, 170(3): 548-563.
19. 王幼辉, 王锡恩, 彭敏, 等. 自噬相关蛋白 Beclin1、p62、Atg5 在浆液性卵巢癌组织中的表达及临床意义. 中华内分泌外科杂志, 2016, 10(4): 329-332.
20. Camargo R, Riccardi D, Ribeiro H, et al. NF-κBp65 and expression of its pro-inflammatory target genes are upregulated in the subcutaneous adipose tissue of cachectic cancer patients. Nutrients, 2015, 7(6): 4465-4479.
21. Kang K F, Wang X W, Chen X W, et al. Beclin 1 and nuclear factor-κBp65 are upregulated in hepatocellular carcinoma. Oncology Letters, 2013, 5(6): 1813-1818.
22. 廖桂周. NIBP 和 NF-κBp65 在胃癌中的表达及其临床意义. 中国医药科学, 2016, 6(14): 27-30.
23. Guo YZ, He P, Feng AM. Effect of curcumin on expressions of NF-κ Bp65,TNF-α and IL-8 in placental tissue of premature birth of infected mice. Asian Pac J Trop Med, 2017, 10(2): 168-171.
24. Zhu J, Yang L, Chen C, et al. NF-κB p65 overexpression promotes bladder cancer cell migration via FBW7-mediated degradation of RhoGDIα protein. Neoplasia, 2017, 19(9): 672-683.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

The expressions and clinical significance of NF-κBp65 and autophagy related proteins Beclin1 and p62 in thyroid papillary carcinoma

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content