Expressions and clinical significance of SDF-1 and CXCR4 in local tissues of perianal abscess_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 HAO Zhi ¹ , ZHEN Hongbo ² , GONG Haifeng ¹

1. Department of General Surgery, Beijing First Hospital of Integrated Chinese and Western Medicine , Beijing 100026, P. R. China;
2. Department of Eye, Beijing Sixth Hospital, Beijing 100007, P. R. China;

Corresponding author：

HAO Zhi, Email: long.2659@163.com

Keywords：

perianal abscess; stromal cell derived factor 1; chemokine receptor-4; clinicopathological feature; recurrence

DOI：

10.7507/1007-9424.201906085

Video：

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Objective To investigate the expressions of stromal cell-derived factor-1 (SDF-1) and chemokine receptor-4 (CXCR4) in local tissues of perianal abscess and their relationships with clinicopathological features and prognosis of patients.Methods A total of 47 patients with perianal abscess (perianal abscess group) and 58 patients with mixed hemorrhoids (mixed hemorrhoids group) were selected for the study. The tissues were collected during the operation. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect the expressions of SDF-1 mRNA and CXCR4 mRNA in local tissues of the two groups, the positive expressions of SDF-1 protein and CXCR4 protein in local tissues were detected by immunohistochemistry, and the relationships between the expressions of SDF-1 and CXCR4 protein and the clinical characteristics, prognosis of patients were analyzed.Results The expression levels of SDF-1 mRNA and CXCR4 mRNA in the perianal abscess group were higher than those in the mixed hemorrhoids group, and the positive rates of SDF-1 protein and CXCR4 protein in the perianal abscess group were higher than those in the mixed hemorrhoids group too (P<0.05). The expressions of SDF-1 protein and CXCR4 protein in perianal abscess tissues were both not related to sex, age, location of abscess, and course of disease (P>0.05), but was related to abscess diameter, healing time, and anal fistula (P<0.05). The non-recurrence rates of SDF-1 protein-negative group and CXCR4 protein-negative group were lower than those of SDF-1 protein-positive group and CXCR4 protein-positive group respectively (P<0.05).Conclusion SDF-1 and CXCR4 molecular are up-regulated in the local tissues of perianal abscess, which are related to the size of abscess, healing time, anal fistula, and recurrence of patients.

Citation： HAO Zhi, ZHEN Hongbo, GONG Haifeng. Expressions and clinical significance of SDF-1 and CXCR4 in local tissues of perianal abscess. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2019, 26(12): 1434-1439. doi: 10.7507/1007-9424.201906085 Copy

1.	陈永乐, 王世霞. 1 330 例肛肠疾病患者发病特征分析. 临床医学研究与实践, 2018, 3(20): 119-120.
2.	焦峰, 仲艳阳, 何秀芝, 等. 高位肛周脓肿切开术后不同引流方式的临床对照研究. 实用医学杂志, 2018, 34(13): 2275-2276, 2279.
3.	Zheng F, Zhang Z, Flamini V, et al. The axis of CXCR4/SDF-1 plays a role in colon cancer cell adhesion through regulation of the AKT and IGF1R signalling pathways. Anticancer Res, 2017, 37(8): 4361-4369.
4.	林旭红, 王丹丹, 王慧超, 等. CXCR4 在溃疡性结肠炎小鼠蛋白 C 系统变化中的作用. 中国病理生理杂志, 2016, 32(10): 1854-1862.
5.	刘瑞, 罗前程, 朱鸿玲, 等. IL-4 和 IL-13 在甲状腺乳头状癌中的表达及其与临床病理特征和预后的关系. 临床与病理杂志, 2018, 38(3): 490-497.
6.	郁俊文, 刘旭元. 一期手术治疗肛周脓肿 120 例. 中国肛肠病杂志, 2012, 32(12): 20.
7.	张旭标, 林涛, 曾永亲. 胶质瘤中间质细胞衍生因子-1 及神经生长因子的表达及临床意义. 河北医药, 2017, 39(15): 2250-2253.
8.	王建平, 杜金林, 蔡成, 等. 肛周脓肿局部组织及外周血中 IL-22 的表达及临床意义. 浙江医学, 2015, 37(11): 958-960, 963.
9.	曹影. 肛周脓肿检测中外周血 C 反应蛋白、纤维蛋白原含量对其诊治的临床意义. 中国肛肠病杂志, 2019, 39(1): 13-14.
10.	陶勇, 杨关根, 裘建明, 等. 人催乳素受体蛋白在肛周脓肿患者中的表达及其与预后的关系. 中华结直肠疾病电子杂志, 2017, 6(6): 488-493.
11.	Huang X, Mao W, Zhang T, et al. Baicalin promotes apoptosis and inhibits proliferation and migration of hypoxia-induced pulmonary artery smooth muscle cells by up-regulating A2a receptor via the SDF-1/CXCR4 signaling pathway. BMC Complement Altern Med, 2018, 18(1): 330-337.
12.	Ando N, Furuichi Y, Kasai S, et al. Chemosensitivity is differentially regulated by the SDF-1/CXCR4 and SDF-1/CXCR7 axes in acute lymphoblastic leukemia with MLL gene rearrangements. Leuk Res, 2018, 75(12): 36-44.
13.	陈艳昕, 刘庆滨, 周颖, 等. Vasohibin-1、PD-1、SDF-1 在结直肠癌患者中的表达及其与临床特征的关系. 癌症进展, 2018, 16(5): 643-646.
14.	周琦, 张宁, 卢芳, 等. 穿山龙总皂苷对痛风性关节炎大鼠关节炎滑膜 IL-1β 及其信号转导通路的影响. 中药药理与临床, 2013, 29(6): 52-57.
15.	Margaritopoulos GA, Antoniou KM, Lasithiotaki I, et al. Expression of SDF-1/CXCR4 axis in bone marrow mesenchymal stem cells derived from rheumatoid arthritis-usual interstitial pneumonia. Clin Exp Rheumatol, 2013, 31(4): 610-611.
16.	宋爽, 李虹, 殷少军, 等. 基质细胞衍生因子-1 在慢性阻塞性肺疾病患者诱导痰中的表达及临床意义. 国际呼吸杂志, 2018, 38(21): 1619-1623.
17.	顾园, 龚攀. SDF-1/CXCR4 轴在溃疡性结肠炎病理过程中的作用. 中国临床研究, 2018, 31(6): 757-759.
18.	张磊, 孙雪竹, 孙景洲. SDF-1/CXCR4 及 VEGF 在结直肠腺癌中的表达及其意义. 中国免疫学杂志, 2015, 31(5): 674-677, 682.
19.	陈志京, 廖山婴, 吴伍林, 等. 趋化因子 CCL20、SDF-1 在大肠癌中的表达及其与淋巴结及肝脏转移的关系. 广东医学, 2018, 39(17): 2598-2601, 2605.
20.	Ramonell KM, Zhang W, Hadley A, et al. CXCR4 blockade decreases CD4⁺ T cell exhaustion and improves survival in a murine model of polymicrobial sepsis. PLoS One, 2017, 12(12): e0188882.
21.	Patry C, Stamm D, Betzen C, et al. CXCR-4 expression by circulating endothelial progenitor cells and SDF-1 serum levels are elevated in septic patients. J Inflamm (Lond), 2018, 15(1): 10-18.
22.	李伟, 邢国辉, 董佃国. 原发性肝癌 SDF-1/CXCR4 轴与血管生成和病灶转移相关性研究. 中华肿瘤防治杂志, 2017, 24(9): 606-610.
23.	Baran M, Çağan Appak Y, Garipcin P, et al. The role of familial mediterranean fever gene mutation in treatment of infantile colitis with resistant perianal fistula. Arch Rheumatol, 2018, 33(4): 473-477.
24.	Weixler B, Renetseder F, Facile I, et al. Phosphorylated CXCR4 expression has a positive prognostic impact in colorectal cancer. Cell Oncol (Dordr), 2017, 40(6): 609-619.
25.	Cotoia A, Mirabella L, Altamura S, et al. Circulating stem cells, HIF-1, and SDF-1 in septic abdominal surgical patients: randomized controlled study protocol. Trials, 2018, 19(1): 179-186.

1. 陈永乐, 王世霞. 1 330 例肛肠疾病患者发病特征分析. 临床医学研究与实践, 2018, 3(20): 119-120.
2. 焦峰, 仲艳阳, 何秀芝, 等. 高位肛周脓肿切开术后不同引流方式的临床对照研究. 实用医学杂志, 2018, 34(13): 2275-2276, 2279.
3. Zheng F, Zhang Z, Flamini V, et al. The axis of CXCR4/SDF-1 plays a role in colon cancer cell adhesion through regulation of the AKT and IGF1R signalling pathways. Anticancer Res, 2017, 37(8): 4361-4369.
4. 林旭红, 王丹丹, 王慧超, 等. CXCR4 在溃疡性结肠炎小鼠蛋白 C 系统变化中的作用. 中国病理生理杂志, 2016, 32(10): 1854-1862.
5. 刘瑞, 罗前程, 朱鸿玲, 等. IL-4 和 IL-13 在甲状腺乳头状癌中的表达及其与临床病理特征和预后的关系. 临床与病理杂志, 2018, 38(3): 490-497.
6. 郁俊文, 刘旭元. 一期手术治疗肛周脓肿 120 例. 中国肛肠病杂志, 2012, 32(12): 20.
7. 张旭标, 林涛, 曾永亲. 胶质瘤中间质细胞衍生因子-1 及神经生长因子的表达及临床意义. 河北医药, 2017, 39(15): 2250-2253.
8. 王建平, 杜金林, 蔡成, 等. 肛周脓肿局部组织及外周血中 IL-22 的表达及临床意义. 浙江医学, 2015, 37(11): 958-960, 963.
9. 曹影. 肛周脓肿检测中外周血 C 反应蛋白、纤维蛋白原含量对其诊治的临床意义. 中国肛肠病杂志, 2019, 39(1): 13-14.
10. 陶勇, 杨关根, 裘建明, 等. 人催乳素受体蛋白在肛周脓肿患者中的表达及其与预后的关系. 中华结直肠疾病电子杂志, 2017, 6(6): 488-493.
11. Huang X, Mao W, Zhang T, et al. Baicalin promotes apoptosis and inhibits proliferation and migration of hypoxia-induced pulmonary artery smooth muscle cells by up-regulating A2a receptor via the SDF-1/CXCR4 signaling pathway. BMC Complement Altern Med, 2018, 18(1): 330-337.
12. Ando N, Furuichi Y, Kasai S, et al. Chemosensitivity is differentially regulated by the SDF-1/CXCR4 and SDF-1/CXCR7 axes in acute lymphoblastic leukemia with MLL gene rearrangements. Leuk Res, 2018, 75(12): 36-44.
13. 陈艳昕, 刘庆滨, 周颖, 等. Vasohibin-1、PD-1、SDF-1 在结直肠癌患者中的表达及其与临床特征的关系. 癌症进展, 2018, 16(5): 643-646.
14. 周琦, 张宁, 卢芳, 等. 穿山龙总皂苷对痛风性关节炎大鼠关节炎滑膜 IL-1β 及其信号转导通路的影响. 中药药理与临床, 2013, 29(6): 52-57.
15. Margaritopoulos GA, Antoniou KM, Lasithiotaki I, et al. Expression of SDF-1/CXCR4 axis in bone marrow mesenchymal stem cells derived from rheumatoid arthritis-usual interstitial pneumonia. Clin Exp Rheumatol, 2013, 31(4): 610-611.
16. 宋爽, 李虹, 殷少军, 等. 基质细胞衍生因子-1 在慢性阻塞性肺疾病患者诱导痰中的表达及临床意义. 国际呼吸杂志, 2018, 38(21): 1619-1623.
17. 顾园, 龚攀. SDF-1/CXCR4 轴在溃疡性结肠炎病理过程中的作用. 中国临床研究, 2018, 31(6): 757-759.
18. 张磊, 孙雪竹, 孙景洲. SDF-1/CXCR4 及 VEGF 在结直肠腺癌中的表达及其意义. 中国免疫学杂志, 2015, 31(5): 674-677, 682.
19. 陈志京, 廖山婴, 吴伍林, 等. 趋化因子 CCL20、SDF-1 在大肠癌中的表达及其与淋巴结及肝脏转移的关系. 广东医学, 2018, 39(17): 2598-2601, 2605.
20. Ramonell KM, Zhang W, Hadley A, et al. CXCR4 blockade decreases CD4⁺ T cell exhaustion and improves survival in a murine model of polymicrobial sepsis. PLoS One, 2017, 12(12): e0188882.
21. Patry C, Stamm D, Betzen C, et al. CXCR-4 expression by circulating endothelial progenitor cells and SDF-1 serum levels are elevated in septic patients. J Inflamm (Lond), 2018, 15(1): 10-18.
22. 李伟, 邢国辉, 董佃国. 原发性肝癌 SDF-1/CXCR4 轴与血管生成和病灶转移相关性研究. 中华肿瘤防治杂志, 2017, 24(9): 606-610.
23. Baran M, Çağan Appak Y, Garipcin P, et al. The role of familial mediterranean fever gene mutation in treatment of infantile colitis with resistant perianal fistula. Arch Rheumatol, 2018, 33(4): 473-477.
24. Weixler B, Renetseder F, Facile I, et al. Phosphorylated CXCR4 expression has a positive prognostic impact in colorectal cancer. Cell Oncol (Dordr), 2017, 40(6): 609-619.
25. Cotoia A, Mirabella L, Altamura S, et al. Circulating stem cells, HIF-1, and SDF-1 in septic abdominal surgical patients: randomized controlled study protocol. Trials, 2018, 19(1): 179-186.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expressions and clinical significance of SDF-1 and CXCR4 in local tissues of perianal abscess

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