Therapeutic Effect on Radioative Lung Injury by Different Doses of Tanshinone ⅡA Microemulsion_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

SunYunhui ¹ , YangXinlei ¹ , MaXuemei ¹ , YangXiaodong ¹ , PingYang ² , YuLian ² ,  BaoWenhua ¹

1. Department of Respiratory Medicine, School of Jiamusi University, Jiamusi, Heilongjiang, 154002, China;
2. ;

Corresponding author：

BaoWenhua, Email: baowenhua3@sina.com

Keywords：

Radiation-induced lung injury; Tanshinone ⅡA microemulsion; Dose; Smad

DOI：

10.7507/1671-6205.2016088

Video：

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Objective To investigate the therapeutic effects of different doses of tanshinone ⅡA microemulsion on radioactive lung injury. Methods Seventy-two Wistar rats were randomly divided into a healthy control group,a model group,a liposome microemulsion treatment group,a tanshinone ⅡA microemulsion high-dose group,a tanshinone ⅡA microemulsion middle-dose group,and a tanshinone ⅡA microemulsion low-dose group.Radiation-induced lung injury model was established by irradiation of radiotherapy instrument.In addition to the control group,other groups received 6MV X radiation with one dosage of 22Gy.Four rats in each group were sacrificed on 7^th,14^th,and 28^th day,respectively.Lung tissues were sampled to analyze the pathological changes by HE staining and the Smad7 mRNA expression by RT-PCR.The level of glutathione(GSH)in peripheral blood was determined by ultraviolet spectrophotometric method. Results In the model group and four treatment groups,lung tissue biopsy showed the pathological changes gradually from pulmonary alveolitis to fibrosis.The level of Smad7 mRNA in lung tissue and GSH in peripheral blood were higher in the high-dose group,the middle-dose group and the low-dose group than those in the model group at all time points(P<0.05),and were highest in the high-dose group.There was no significant differences in the level of Smad7 mRNA in lung tissue and GSH in peripheral blood between the liposome microemulsion treatment group and the middle-dose group. Conclusion Tanshinone ⅡA microemulsion has treatment effect on lung injury in a dose dependent manner.

Citation： SunYunhui, YangXinlei, MaXuemei, YangXiaodong, PingYang, YuLian, BaoWenhua. Therapeutic Effect on Radioative Lung Injury by Different Doses of Tanshinone ⅡA Microemulsion. Chinese Journal of Respiratory and Critical Care Medicine, 2016, 15(4): 374-378. doi: 10.7507/1671-6205.2016088 Copy

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17.	雷章, 卢宏达,卢驰,等.还原型谷胱甘肽对大鼠急性放射性肺损伤炎症的影响.中国医院药学杂志,2014,34:1449-1453.
18.	徐婷贞, 杨起初,安娇娇,等.虎杖对放射性肺损伤大鼠TGF-β1/Smad蛋白的影响.中华中医药学刊,2015,33:1421-1425.

1. 毛艳, 刘文其.放射性肺损伤防护的研究进展.肿瘤,2012,32:389-394.
2. 史毓杰, 曾抗,李国锋,等.鬼臼毒素固体脂质纳米粒的制备及对人表皮细胞体外增殖的影响.解放军医学杂志,2008,33:42-44.
3. 毕茹. 胰岛素固体脂质纳米粒粉雾剂的制备.西北药学杂志,2011,26:204-206.
4. 王新春, 侯世祥,阳长明,等.大黄复方液体喷雾剂中挥发油微乳与相图的应用.中成药,2004,26:8-11.
5. 串俊兰, 龚涛,孙逊,等.利福平固体脂质纳米粒的体外细胞摄取研究.华西药学杂志,2011,26:223-225.
6. 冯欣, 张须学,姬诚.丹参酮亚微乳制剂的制备工艺和质量评价.中国医药指南,2012,10:51-53.
7. 丛军兹, 王丹,周杨,等.丹参酮ⅡA纳米结构脂质载体的制备及体外评价.牡丹江医学院学报,2015,36:27-29.
8. 吴丽明, 黄国红,李庆国.高效液相色谱法测定吉非替尼的含量.当代医学,2011,17:23-25.
9. Nakao A,Fujii M,Matsumura R,et al.Transient gene transfer and expression of Smad7 prevents bleomycin induced lung fibrosis in mice.J Clin Invest,1999,104:5-11.
10. 刘双秀, 郭和宗,冯书凤,等.丹参酮治疗放射性肺炎临床观察.中医学报,2014,29:1411-1412.
11. 李广虎, 李志平,徐泳,等.丹参酮ⅡA对放射性肺纤维化防治作用的实验研究.中华放射肿瘤学杂志,2006,15:50-54.
12. 周雨田, 徐军.反义内皮素转换酶核酸表达质粒纳米粒所致内皮素的减少对哮喘小鼠气道高反应性的影响.实用医院临床杂志,2013,10:28-31.
13. 刘佳. 壳聚糖纳米粒包裹反义内皮素转换酶核酸表达质粒与哮喘气道高反应性.中国组织工程研究,2015,19:6913-6917.
14. 赵斐. 丹参酮IIA对TGF-β1诱导的HK-2细胞TGF-β1/Smads信号通路影响的实验研究[学位论文].北京中医药大学,2014.
15. 李瑞珊. 卵磷脂注射溶脂的实验研究[学位论文].南方医科大学,2010.
16. 陈志霄, 周道安,赵国旗.放射性肺损伤的研究进展.中国肿瘤,2010,19:53-58.
17. 雷章, 卢宏达,卢驰,等.还原型谷胱甘肽对大鼠急性放射性肺损伤炎症的影响.中国医院药学杂志,2014,34:1449-1453.
18. 徐婷贞, 杨起初,安娇娇,等.虎杖对放射性肺损伤大鼠TGF-β1/Smad蛋白的影响.中华中医药学刊,2015,33:1421-1425.

Chinese Journal of Respiratory and Critical Care Medicine

Therapeutic Effect on Radioative Lung Injury by Different Doses of Tanshinone ⅡA Microemulsion

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