Expression of Cytokine-Induced Neutrophil Chemoattractant-1 in Rats with Transfusion-Related Acute Lung Injury_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

LiangYinghong , GongYanjie , LiuJia ,  WeiMing , TuLing , ZhangYihua , YangLu

Department of Transfusion, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052, China;

Corresponding author：

WeiMing, Email: gushiweiming@126.com

Keywords：

Transfusion-related acute lung injury; Cytokine-induced neutrophil chemoattractant; Sprague-Dawley rats

DOI：

10.7507/1671-6205.2016089

Video：

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Objective To study the expression of cytokine-induced neutrophil chemoattractant-1(CINC-1)in rats with transfusion-related acute lung injury(TRALI),explore its possible role in the pathogenesis of TRALI. Methods Sixty Sprague-Dawley rats were randomly divided into a normal control group with sham operation,a positive control group with ALI induced by intravenous infusion of lipopolysaccharide(5 mg/kg),and a TRALI group treated by intraperitoneal injection of LPS 2h before the transfusion of human plasma (1mL),a LPS control group treated by intraperitoneal injection of LPS 2h before the transfusion of normal saline(1mL).The reverse transcription-polymerase chain (RT-PCR)was used to detect CINC-1 mRNA.The level of CINC-1 in lung tissue homogenate was measured by ELISA.Morphological changes of the lung tissue were observed under light microscope.Myeloperoxidase (MPO)in lung homogenate and wet lung weight to dry lung weight ratio (W/D)were observed.The number of cells and the percentage of polymorphonuclear neutrophil (PMN)in Bronchoalveolar lavage fluid (BALF)were also compared. Results Compared with the normal control group and the LPS control group,the expression of CINC-1 protein and CINC-1 mRNA were increased significantly in lung of the positive control group and the TRALI group(P<0.05).The number of cells and the percentage of PMN in BALF of the TRALI group [(310.63±76.67)×10⁶/L and (33.57±11.51)%] were significantly higher than those in BALF of the normal control group [(101.36±63.83)×10⁶/L and (9.87±3.56)%](P<0.05).Tissue water content and MPO activity in the TRALI group were significantly higher than those in the normal control group (P<0.05). Conclusion Expression of CINC-1 protein and CINC-1 mRNA are increased in the rat lung with TRALI and PMN infiltration in lung tissue,which suggests CINC-1 participate in the process of the PMN and endothelial cell adhesion and may play an important role in the pathogeneses of TRALI.

Citation： LiangYinghong, GongYanjie, LiuJia, WeiMing, TuLing, ZhangYihua, YangLu. Expression of Cytokine-Induced Neutrophil Chemoattractant-1 in Rats with Transfusion-Related Acute Lung Injury. Chinese Journal of Respiratory and Critical Care Medicine, 2016, 15(4): 379-383. doi: 10.7507/1671-6205.2016089 Copy

1.	王丽, 张春民,张金波.检测输血前4项传染性指标对受血者临床价值与应用.国际检验医学杂志,2010,31:104.
2.	Anne F,Eder-Beth A,Dy-Jaime M,et al.The residual risk of transfusion-related acute lung injury at the American Red Cross (2008-2011):limitations of a predominantly male-donor plasma mitigation strategy.Transfusion,2013,53:1442-1449.
3.	廖刃, 刘进.围手术期血液管理:由限制性输血走向个体化输血.中华医学杂志,2014,94:481-482.
4.	Hanane EL,Kenz P,Vander L.Transfusion-related acute lung injury.Euro J Anaesth,2014,31:345-350.
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7.	李玮, 谢如锋,范华骅.中性粒细胞在输血相关急性肺损伤中的作用.中国输血杂志,2012,25:1335-1338.
8.	曹瑞旗, 吴文浩,李晓松,等.罗哌卡因胸段硬膜外阻滞对油酸致猪急性肺损伤时PMN及ICAM-1的影响.河北医药,2009,25:2709-2711.
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11.	Nicholson SE,Johnson RA,John G,et al.Transfusion-related acute lung injury in a rat model of trauma-hemorrhage.J Trau Inj,2011,70:466-471.
12.	Mikawa K,Nishina K,Takaa Y,et al.ONO-1714,a nitric oxide synthase inhibitor,attenuated endotoxin-induced acute lung injury in rabbits.Anesth Anolg,2003,97:1751-1755.
13.	Mark R,Looney N,Roubinian O,et al.Prospective study on the clinical course and outcomes in transfusion-related acute lung injury.Crit Care Med,2014,42:1676-1687.
14.	Silliman CC.The two-event model of transfusion-related acute lung injury.Crit Care Med,2006,34(5 Suppl):S124-S131.
15.	Looney MR,Su X,Van Ziffle JA,et al.Neutrophils and their Fcγ receptors are essential in a mouse model of transfusion-related acute lung injury.J Clin Invest,2006,116:1615-1623.
16.	Takahashi D,Fujihara M,Azuma H,et al.Stimulation of human neutrophils with sera containing HLA Class I alloantibody causes preferential degranulation of azurophilic granules and secretory vesicles.Vox Sang,2010,98:560-566.
17.	Bernadette F,West-Christopher C,Silliman CC.Transfusion-related acute lung injury:advances in understanding the role of proinflammatory mediators in its genesis.Expert Rev Hematol,2013,6:265-276.
18.	Gottschall JL,Triulzi DJ,Curtis B,et al.The frequency and specificity of human neutrophil antigen antibodies in a blood donor population.Transfusion,2011,51:820-827.
19.	Sayah DM,Looney MR, Toy P,et al.Transfusion reactions:newer concepts on the pathophysiology,incidence,treatment,and prevention of transfusion-related acute lung injury.Crit Care Clin,2012,28:363-372.
20.	Silliman CC,Bercovitz RS,Khan SY,et al.Antibodies to the HLA-A2 antigen prime neutrophils and serve as the second event in an in vitro model of transfusion-related acute lung injury.Vox Sanguinis,2014,107:76-82.
21.	Leanne C,Amandeep S,Gregory A,et al.Electronic health record surveillance algorithms facilitate the detection of transfusion-related pulmonary complications.Transfusion,2013,53:1205-1216.
22.	夏长江, 孟革,赵建,等.中性粒细胞在急性肺损伤中的作用机制研究进展.国际药学研究杂志,2009,36:418-422.
23.	Takaishi K,Ohtsuka T,Tsuneyoshi S,et al.Inhibition of the production of rat cytokine-induced neutrophil chemoattractant (CINC)-1,a member of the interleukin-8 family,by adenovirus-mediated overexpression of IκBα.J Biochem,2000,127:511-516.

1. 王丽, 张春民,张金波.检测输血前4项传染性指标对受血者临床价值与应用.国际检验医学杂志,2010,31:104.
2. Anne F,Eder-Beth A,Dy-Jaime M,et al.The residual risk of transfusion-related acute lung injury at the American Red Cross (2008-2011):limitations of a predominantly male-donor plasma mitigation strategy.Transfusion,2013,53:1442-1449.
3. 廖刃, 刘进.围手术期血液管理:由限制性输血走向个体化输血.中华医学杂志,2014,94:481-482.
4. Hanane EL,Kenz P,Vander L.Transfusion-related acute lung injury.Euro J Anaesth,2014,31:345-350.
5. Flesch BK,Petershofen EK,Bux J.TRALI-new challenges for histocompatibility and immunogenetics in transfusion medicine.Tissue Antigens,2011,78:1-7.
6. Muschtcr S,Bcrthold T,Urcinachcr A.Developments in the definition and clinical impact of human neutrophil antigens.Curr Opin Hematol,2011,18:452-160.
7. 李玮, 谢如锋,范华骅.中性粒细胞在输血相关急性肺损伤中的作用.中国输血杂志,2012,25:1335-1338.
8. 曹瑞旗, 吴文浩,李晓松,等.罗哌卡因胸段硬膜外阻滞对油酸致猪急性肺损伤时PMN及ICAM-1的影响.河北医药,2009,25:2709-2711.
9. 李丽玮, 花紫菱,张慧箴,等.输血相关急性肺损伤大鼠模型建立及肺组织病理研究.中国输血杂志,2012,25:1121-1124.
10. Gilson CR,Kraus TS,Hod EA,et al.A novel mouse model of red blood cell storage and post-transfusion in vivo survival.Transfusion,2009,49:1546-1553.
11. Nicholson SE,Johnson RA,John G,et al.Transfusion-related acute lung injury in a rat model of trauma-hemorrhage.J Trau Inj,2011,70:466-471.
12. Mikawa K,Nishina K,Takaa Y,et al.ONO-1714,a nitric oxide synthase inhibitor,attenuated endotoxin-induced acute lung injury in rabbits.Anesth Anolg,2003,97:1751-1755.
13. Mark R,Looney N,Roubinian O,et al.Prospective study on the clinical course and outcomes in transfusion-related acute lung injury.Crit Care Med,2014,42:1676-1687.
14. Silliman CC.The two-event model of transfusion-related acute lung injury.Crit Care Med,2006,34(5 Suppl):S124-S131.
15. Looney MR,Su X,Van Ziffle JA,et al.Neutrophils and their Fcγ receptors are essential in a mouse model of transfusion-related acute lung injury.J Clin Invest,2006,116:1615-1623.
16. Takahashi D,Fujihara M,Azuma H,et al.Stimulation of human neutrophils with sera containing HLA Class I alloantibody causes preferential degranulation of azurophilic granules and secretory vesicles.Vox Sang,2010,98:560-566.
17. Bernadette F,West-Christopher C,Silliman CC.Transfusion-related acute lung injury:advances in understanding the role of proinflammatory mediators in its genesis.Expert Rev Hematol,2013,6:265-276.
18. Gottschall JL,Triulzi DJ,Curtis B,et al.The frequency and specificity of human neutrophil antigen antibodies in a blood donor population.Transfusion,2011,51:820-827.
19. Sayah DM,Looney MR, Toy P,et al.Transfusion reactions:newer concepts on the pathophysiology,incidence,treatment,and prevention of transfusion-related acute lung injury.Crit Care Clin,2012,28:363-372.
20. Silliman CC,Bercovitz RS,Khan SY,et al.Antibodies to the HLA-A2 antigen prime neutrophils and serve as the second event in an in vitro model of transfusion-related acute lung injury.Vox Sanguinis,2014,107:76-82.
21. Leanne C,Amandeep S,Gregory A,et al.Electronic health record surveillance algorithms facilitate the detection of transfusion-related pulmonary complications.Transfusion,2013,53:1205-1216.
22. 夏长江, 孟革,赵建,等.中性粒细胞在急性肺损伤中的作用机制研究进展.国际药学研究杂志,2009,36:418-422.
23. Takaishi K,Ohtsuka T,Tsuneyoshi S,et al.Inhibition of the production of rat cytokine-induced neutrophil chemoattractant (CINC)-1,a member of the interleukin-8 family,by adenovirus-mediated overexpression of IκBα.J Biochem,2000,127:511-516.

Chinese Journal of Respiratory and Critical Care Medicine

Expression of Cytokine-Induced Neutrophil Chemoattractant-1 in Rats with Transfusion-Related Acute Lung Injury

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