Expression of Pokemon Protein Predicts The Surgical Molecular Margin in Upper Gastric Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YANG Wei ¹ , CAI Xinxin ² ,  QU Hongyan ¹ , LI Yanfeng ¹ , ZHANG Yongle ¹

1.Department of Gastrointestinal Surgery， The Third Affiliated Hospital， Harbin Medical University， Harbin 150081， Heilongjiang Province， China;;
2.Department of Anes-thesiology， Graduate School， Guilin Medical College， Guilin 514000， Guangxi Zhuang Autonomous Region， China;

Corresponding author：

QU Hongyan, Email: quhongyan69@163.com

Keywords：

POK erythroid myeloid ontogenic factor; Upper gastric cancer; Molecular margin

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Abstract Full text Figures/Tables Video References Cited by

Objective 　To investigate the safe distance of upper gastric cancer by pokemon protein.
Methods 　In 60 cases of upper gastric cancer， specimens were get from tumors’ tissue and every 0.5 cm from the proximal of tumor margin beginning at the 1.0 cm to distal. Western blot method was used to test the expression of pokemon protein.
Results 　There were 38 cases （63.3%） positively expressed pokemon protein. Compared with the center of tumor， the positive expression rates of pokemon protein at every sites of 2.5-5.0 cm from margin were lower （P＜0.003）， but didn’t signi-
ficantly differed at every sites of 1.0-2.0 cm from margin （P≥0.003）. When the incisal margin distance of more than 3.5 cm of both sides， the positive expression rate of pokemon protein were 0. There were no significant difference between the corresponding sites of the upper resection margin and lower resection margin （P＞0.05）.
Conclusions 　The results show that 3.5 cm may be the molecular margin basing on the positive expression of pokemon protein in the surgical margin of upper gastric cancer， which may have guiding significance to the surgical margin.

Citation： YANG Wei,CAI Xinxin,QU Hongyan,LI Yanfeng,ZHANG Yongle. Expression of Pokemon Protein Predicts The Surgical Molecular Margin in Upper Gastric Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2013, 20(12): 1386-1390. doi: Copy

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1. Kim JH， Park SS， Kim J， et al. Surgical outcomes for gastric cancer in the upper third of the stomach[J]. World J Surg， 2006， 30(10)：1870-1876.
2. Li N， Wang J， Shen S， et al. Expression of p53， Ki-67 and c-Mycproteins is predictive of the surgical molecular margin in colorectalcarcinoma[J]. Pathol Oncol Res， 2011， 17(3)：479-487.
3. 曹国春，王会，黄亚玲，等. Pokemon在人贲门癌中的表达及其意义[J]. 南京大学学报：自然科学版， 2010， 46(1)：108-113.
4. Maeda T， Hobbs RM， Pandolfi PP. The transcription factor Pokemon：a new key player in cancer pathogenesis[J]. Cancer Res， 2005， 65(19)：8575-8578.
5. Agrawal A， Yang J， Murphy RF， et al. Regulation of the p14ARF-Mdm2-p53 pathway：an overview in breast cancer[J]. Exp Mol Pathol， 2006， 81(2)：115-122.
6. Apostolopoulou K， Pateras IS， Evangelou K， et al. Gene amplification is a relatively frequent event leading to ZBTB7A (Pokemon) overexpression in non-small cell lung cancer[J]. J Pathol， 2007， 213(3)：294-302.
7. 韩晓峰，李月锋，曲宏岩. Pokemon、Ki-67蛋白在胃癌中的表达和临床意义[J]. 实用肿瘤学杂志， 2011， 25(3)：219-222.
8. 李月锋，曲宏岩，张东欣. Pokemon、Ki-67及Livin蛋白在胃癌中的表达及意义[J]. 中国现代普通外科进展， 2012， 15(3)：179-182.
9. 王洁贞. 医学统计学[J]. 第3版. 人民卫生出版社， 2009：111-130.
10. Ji J， Hemminki K. Second gastric cancers among patients with primary sporadic and familial cancers in Sweden[J]. Gut， 2006， 55(6)：896-898.
11. Hotta K， Oyama T， Akamatsu T， et al. A comparison of outcomesof endoscopic submucosal dissection (ESD) for early gastric neoplasms between high-volume and low-volume centers：multi-center retrospective questionnaire study conducted by the Nagano ESD Study Group[J]. Intern Med， 2010， 49(4)：253-259.
12. Ferlay J， Shin HR， Bray F， et al. Estimates of worldwide burdenof cancer in 2008：GLOBOCAN 2008[J]. Int J Cancer， 2010， 127(12)：2893-2917.
13. 田洪鹏，王继见. 新辅助化疗对胃癌5年生存率影响的Meta分析[J]. 重庆医学， 2012， 41(2)：142-144.
14. Bateman AC， Carr NJ， Warren BF. The retroperitoneal surface in distal caecal and proximal ascending colon carcinoma：thecinderella surgical margin?[J]. J Clin Pathol， 2005， 58(4)：426-428.
15. Braakhuis BJ， Bloemena E， Leemans CR， et al. Molecular analysis of surgical margins in head and neck cancer：more than a marginal issue[J]. Oral Oncol， 2010， 46(7)：485-491.
16. Maeda T， Hobbs RM， Merghoub T， et al. Role of the proto-oncogene Pokemon in cellular transformation and ARF repression[J]. Nature， 2005， 433(7023)：278-285.
17. Calabrò V， Mansueto G， Santoro R， et al. Inhibition of p63 transcriptional activity by p14ARF：functional and physical link between human ARF tumor suppressor and a member of the p53 family[J]. Mol Cell Biol， 2004， 24(19)：8529-8540.
18. Jeon BN， Yoo JY， Choi WI， et al. Proto-oncogene FBI-1 (Pokemon/ZBTB7A) represses transcription of the tumor suppressor Rbgene via binding competition with Sp1 and recruitment of corepre-ssors[J]. J Biol Chem， 2008， 283(48)：33199-33210.
19. Yoon HG， Chan DW， Reynolds AB， et al. N-CoR mediates DNA methylation-dependent repression through a methyl CpG binding protein Kaiso[J]. Mol Cell， 2003， 12(3)：723-734.
20. Goldenberg D， Harden S， Masayesva BG， et al. Intraoperative molecular margin analysis in head and neck cancer[J]. Arch Otolaryngol Head Neck Surg， 2004， 130(1)：39-44.
21. van Houten VM， Leemans CR， Kummer JA， et al. Molecular diagnosis of surgical margins and local recurrence in head and neck cancer patients：a prospective study[J]. Clin Cancer Res， 2004， 10(11)：3614-3620.
22. Masasyesva BG， Tong BC， Brock MV， et al. Molecular margin analysis predicts local recurrence after sublobar resection of lung cancer[J]. Int J Cancer， 2005， 113(6)：1022-1025.
23. Yang B， Gao YT， Du Z， et al. Methylation-based molecular margin analysis in hepatocellular carcinoma[J]. Biochem Biophys Res Commun， 2005， 338(3)：1353-1358.
24. 李乃卿. 胃癌外科治疗进展[J]. 医学理论与实践， 1999， 12(10)：563-564.
25. 吉巍巍. 贲门癌手术径路与疗效分析[J]. 肿瘤基础与临床， 2010， 23(3)：244-245.
26. 方倩，李威. 胃上部癌的临床病理特点及术式比较[J]. 岭南现代临床外科， 2010， 10(5)：326-328.
27. 徐惠绵，张凡. 进展期胃上部癌淋巴结清除及胃切除范围[J]. 中国实用外科杂志， 2001， 67(7)：697-703.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of Pokemon Protein Predicts The Surgical Molecular Margin in Upper Gastric Cancer

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