Up-Regulation of Collagen Expression in Hepatic Stellate Cells by F Protein of Hepatitis C Virus_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WANG Ganlu ¹ ,  SHAO Shengwen ² , XU Baiying ² , LU Tianbao ² , DUAN Jin ² , GU Fuping ²

1.Department of General Surgery, Baoan District People’s Hospital of Shenzhen, Shenzhen 518101, Guangdong Province, China;;
2.Medical School Innovation Laboratory, Huzhou Teachers College, Huzhou 313000, Zhejiang Province, China;

Corresponding author：

SHAO Shengwen, Email: shashe98@163.com

Keywords：

Hepatitis C virus; F protein; Hepatic stellate cells; Collagen

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Objective To investigate the effect of hepatitis C virus (HCV) F protein on proliferation and collagen expression of hepatic stellate cells. Methods After pcDNA3.1-f plasmid containing HCV f gene or empty pcDNA3.1 plasmid was transfected hepatic stellate cells LX2 by liposome, LX-f or LX-p cells were obtained by G418 screening. The proliferation of LX-f or LX-p cells was analyzed by MTT, and the contents of collagen type Ⅰand Ⅲ secreted by LX-f or LX-p cells were detected by ELISA. Results After 24 h cultivation, the proliferation rate of LX-f cells was higher than that of LX-p cells at each time point （P lt;0.01）. After 48 h cultivation, the contents of collagen typeⅠand Ⅲ secreted by LX-f were (25.89±0.42) ng/ml and (18.21±0.49) ng/ml, which was significantly higher than those of LX-p cells 〔(22.65±0.49) ng/ml and (15.29±0.62) ng/ml〕, P lt;0.01. Conclusion HCV F protein is able to promote proliferation of hepatic stellate cells, and up-regulate the excretion of collagen type Ⅰand Ⅲ in those cells, which induces hepatic fibrosis.

Citation： WANG Ganlu ,SHAO Shengwen,XU Baiying,LU Tianbao,DUAN Jin,GU Fuping. Up-Regulation of Collagen Expression in Hepatic Stellate Cells by F Protein of Hepatitis C Virus. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2010, 17(12): 1258-1260. doi: Copy

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18.	Svegliati-Baroni G, De Minicis S, Marzioni M. Hepatic fibrogenesis in response to chronic liver injury: novel insights on the role of cell-to-cell interaction and transition [J]. Liver Int, 2008; 28(8): 1052-1064.
19.	Hatok J, Babusikova E, Matakova T, et al. In vitro assays for the evaluation of drug resistance in tumor cells [J]. Clin Exp Med, 2009; 9(1): 1-7.
20.	刘颖, 梁惠, 苏爱, 等. 海兔素对人胃癌细胞SGC-7901增殖和凋亡的影响 [J]. 中国药理学通报, 2010; 26(3): 333-337.

1. Choo QL, Kuo G, Weiner AJ, et al. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome [J]. Science, 1989; 244(4902): 359-362.
2. Sklan EH, Charuworn P, Pang PS, et al. Mechanisms of HCV survival in the host [J]. Nat Rev Gastroenterol Hepatol, 2009; 6(4): 217-227.
3. Lonardo A, Loria P, Adinolfi LE, et al. Hepatitis C and steatosis: a reappraisal [J]. J Viral Hepat, 2006; 13(2): 73-80.
4. 中华医学会肝病学分会、中华医学会传染病与寄生虫病学分会. 丙型肝炎防治指南 [J]. 中华肝脏病杂志, 2004; 12(4): 194-198.
5. Rosenberg S. Recent advances in the molecular biology of hepatitis C virus [J]. J Mol Biol, 2001; 313(3): 451-464.
6. Castello G, Scala S, Palmieri G, et al. HCV-related hepatocellular carcinoma: From chronic inflammation to cancer [J]. Clin Immunol, 2010; 134(3): 237-250.
7. Zhu Z, Wilson AT, Gopalakrishna K, et al. Hepatitis C virus core protein enhances Telomerase activity in Huh7 cells [J]. J Med Virol, 2010; 82(2): 239-248.
8. Xu Z, Choi J, Yen TS, et al. Synthesis of a novel hepatitis C virus protein by ribosomal frameshift [J]. EMBO J, 2001; 20(14): 3840-3848.
9. Branch AD, Stump DD, Gutierrez JA, et al. The hepatitis C virus alternate reading frame (ARF) and its family of novel products: the alternate reading frame protein/F-protein, the double-frameshift protein, and others [J]. Semin Liver Dis, 2005; 25(1): 105-117.
10. Vassilaki N, Mavromara P. Two alternative translation mechanisms are responsible for the expression of the HCV ARFP/F/core+1 coding open reading frame [J]. J Biol Chem, 2003; 278(42): 40503-40513.
11. Boulant S, Becchi M, Penin F, et al. Unusual multiple recoding events leading to alternative forms of hepatitis C virus core protein from genotype 1b [J]. J Biol Chem, 2003; 278(46): 45785-45792.
12. Gao JH, He ZJ, Wang Q, et al. Low expression of S100P associated with paclitaxel resistance in ovarian cancer cell line [J]. Chin Med J (Engl), 2008; 121(16): 1563-1568.
13. Jiao J, Friedman SL, Aloman C. Hepatic fibrosis [J]. Curr Opin Gastroenterol, 2009; 25(3): 223-229.
14. Marra F, Aleffi S, Galastri S, et al. Mononuclear cells in liver fibrosis [J]. Semin Immunopathol, 2009; 31(3): 345-358.
15. Ghiassi-Nejad Z, Friedman SL. Advances in antifibrotic therapy [J]. Expert Rev Gastroenterol Hepatol, 2008; 2(6): 803-816.
16. Friedman SL. Hepatic stellate cells: protean, multifunctional, and enigmatic cells of the liver [J]. Physiol Rev, 2008; 88(1): 125-172.
17. Kordes C, Sawitza I, Hussinger D. Hepatic and pancreatic stellate cells in focus [J]. Biol Chem, 2009; 390(10): 1003-1012.
18. Svegliati-Baroni G, De Minicis S, Marzioni M. Hepatic fibrogenesis in response to chronic liver injury: novel insights on the role of cell-to-cell interaction and transition [J]. Liver Int, 2008; 28(8): 1052-1064.
19. Hatok J, Babusikova E, Matakova T, et al. In vitro assays for the evaluation of drug resistance in tumor cells [J]. Clin Exp Med, 2009; 9(1): 1-7.
20. 刘颖, 梁惠, 苏爱, 等. 海兔素对人胃癌细胞SGC-7901增殖和凋亡的影响 [J]. 中国药理学通报, 2010; 26(3): 333-337.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Up-Regulation of Collagen Expression in Hepatic Stellate Cells by F Protein of Hepatitis C Virus

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