Objective To investigate the expression of caspase-3 in xenograft that was treated with targeted therapy with magnetic nanoparticles in nude mice. Methods QBC939 cell lines were injected into nude mice subcutaneously to establish the model of human cholangiocarcinoma xenograft. After two weeks of tumor inoculation, the animal models were divided randomly into 4 groups: group A received placebo (sodium chloride), group B were treated with magnetic nanoparticles (250 mg/kg), group C were treated with magnetic nanoparticles (150 mg /kg) combined with inner-stent, group D with magnetic nanoparticles (250 mg /kg) combined with inner-stent (the inner-stent was used to generate the magnetic targeting effect). The 21th day after treatment, expression of caspase-3 in tumor cells of each groups were measured with histochemical method and RT-PCR. Results The quantity of caspase-3 in tumor cells that were treated with magnetic nanoparticles (250 mg/kg) combined with inner-stent was the most (P＜0.05), and the quantity of caspase-3 in cells of group C was significantly more than that of the other two groups (P＜0.05). While the quantity of caspase-3 in group B was more than that of the control group(P＜0.05). Conclusion The use of magnetic nanoparticles combined with inner-stent may increase the expression of caspase-3, and the expression is dose-dependent with magnetic nanoparticles.
Citation：
LI Hong,ZHENG Jianwei,TANG Tao,ZOU Shengquan.. Expression of Caspase-3 in Targeted Therapy with Magnetic Nanoparticle in Cholangiocarcinoma Xenograft of Nude Mice. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(6): 685-688. doi:
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- 2. Aoyagi T, Okano T. Targeting of anticancer drug using intelligent polymers [J]. Nippon Rinsho, 1998; 56(3)∶644.
- 3. Senyei AE, Widder KJ. Drug targeting: magnetically responsive albumin microspheres——a review of the system to date [J]. Gynecol Oncol, 1981; 12(1)∶1.
- 4. 吕 军, 杨连粤, 杨建青. 5-氟尿嘧啶诱导肝癌细胞凋亡过程中 caspase-3活性变化的研究 [J]. 中华实验外科杂志， 2000； 17（5）∶401.
- 5. Widder KJ, Marino PA, Morris RM, et al. Selective targeting of magnetic albumin microspheres to the Yoshida sarcoma: ultrastructural evaluation of microsphere disposition [J]. Eur J Cancer Clin Oncol, 1983; 19(1)∶141.
- 6. Zou W, Zeng J, Zhou M, et al. Involvement of caspase-3 and p38 mitogen-activated protein kinase in cobalt chloride-induced apoptosis in PC12 cells [J]. J Neurosci Res, 2002; 67(6)∶837.
- 7. Fernandes-Alnemri T, Litwack G, Alnemri ES. CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme [J].J Biol Chem, 1994; 269(49)∶30761.
- 8. Cosulich SC, Savory PJ, Clarke PR. Bcl-2 regulates amplification of caspase activation by cytochrome c[J]. Curr Biol, 1999; 9(3)∶147.
- 9. Yeh JH, Hsu SC, Han SH, et al. Mitogen-activated protein kinase kinase antagonized fas-associated death domain protein-mediated apoptosis by induced FLICE-inhibitory protein expression [J]. J Exp Med, 1998; 188(10)∶1795.
