Effect of Hepatocyte Growth Factor/Scatter Factor on Apoptosis of Colorectal Cancer Cells Induced with Curcumin_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LI Hongwu , SHAN Jixian.

Department of Oncology, The First Affiliated Hospital of China Medical University, Shenyang 110001, China;

Corresponding author：

Keywords：

Hepatocyte growth factor Curcumin Colorectal cancer cell Apoptosis

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【Abstract】ObjectiveTo explore the effect of hepatocyte growth factor/scatter factor (HGF/SF) on apoptosis of colorectal cancer cells induced with curcumin.
MethodsMTT assay was used to evaluate the cytotoxicity of curcumin to colorectal cancer cells. Flow cytometry was used to detect the antiapoptosis effect of HGF.
ResultsFlow cytometry showed only 64 μg/ml curcumin could play the proliferationinhibiting role in Caco-2 cells leading to their apoptosis; at the same time, different concentrations of HGF could antagonize this inhibitory effect resulting in the decrease of apoptosis, but HGF worked without a concentration-dependent manner. The study on MAPK pathway showed that the protective effect of HGF on the apoptosis of Caco-2 cells was not influenced by inhibiting p42/p44 MAPK and p38 MAPK pathway.
ConclusionHGF/SF antagonizes the apoptosis of Caco-2 cells induced with curcumin, but MAPK signaling pathway might not participate in this process.

Citation： LI Hongwu,SHAN Jixian.. Effect of Hepatocyte Growth Factor/Scatter Factor on Apoptosis of Colorectal Cancer Cells Induced with Curcumin. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(1): 74-76. doi: Copy

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6.	Weidner KM, Di Cesare S, Sachs M, et al. Interaction between Gab1 and the cMet receptor tyrosine kinase is responsible for epithelial morphogenesis [Ｊ]. Nature， 1996; 384(6605)∶173.
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8.	Huang TS, Lee SC, Lin JK. Suppression of cJun/AP1 activation by an inhibitor of tumor promotion in mouse fibroblast cells [Ｊ]. Proc Natl Acad Sci USA， 1991; 88(12)∶5292.
9.	Samaha HS, Kelloff GJ, Steele V, et al. Modulation of apoptosis by sulindac, curcumin, phenylethyl3methylcaffeate, and 6phenylhexyl isothiocyanate: apoptotic index as a biomarker in colon cancer chemoprevention and promotion [Ｊ]. Cancer Res， 1997; 57(7)∶1301.
10.	Huang MT, Lou YR, Xie JG, et al. Effect of dietary curcumin and dibenzoylmethane on formation of 7,12dimethylbenz [a] anthraceneinduced mammary tumors and lymphomas/leukemias in Sencar mice [Ｊ]. Carcinogenesis， 1998; 19(9)∶1697.
11.	Chen H, Zhang ZS, Zhang YL, et al. Curcumin inhibits cell proliferation by interfering with the cell cycle and inducing apoptosis in colon carcinoma cells [Ｊ]. Anticancer Res， 1999; 19(5A)∶3675.
12.	Moragoda L, Jaszewski R, Majumdar AP. Curcumin induced modulation of cell cycle and apoptosis in gastric and colon cancer cells [Ｊ]. Anticancer Res， 2001; 21(2A)∶873.
13.	Ishiki Y, Ohnishi H, Muto Y, et al. Direct evidence that hepatocyte growth factor is a hepatotrophic factor for liver regeneration and has a potent antihepatitis effect in vivo [Ｊ]. Hepatology， 1992; 16(5)∶1227.
14.	Xiao GH, Jeffers M, Bellacosa A, et al. Antiapoptotic signaling by hepatocyte growth factor/Met via the phosphatidylinositol 3kinase/Akt and mitogenactivated protein kinase pathways [Ｊ]. Proc Natl Acad Sci USA， 2001; 98(1)∶247.

1. Montesano R, Matsumoto K, Nakamura T, et al. Identification of a fibroblastderived epithelial morphogen as hepatocyte growth factor [Ｊ]. Cell， 1991; 67(5)∶901.
2. Nakamura T, Nishizawa T, Hagiya M, et al. Molecular cloning and expression of human hepatocyte growth factor [Ｊ]. Nature， 1989; 342(6248)∶440.
3. Rubin JS, Bottaro DP, Aaronson SA. Hepatocyte growth factor/scatter factor and its receptor, the cmet protooncogene product [Ｊ]. Biochim Biophys Acta， 1993; 1155(3)∶357.
4. Stoker M, Gherardi E, Perryman M, et al. Scatter factor is a fibroblastderived modulator of epithelial cell mobility [Ｊ]. Nature， 1987; 327(6119)∶239.
5. Jeffers M, Rong S, Vande Woude GF. Enhanced tumorigenicity and invasionmetastasis by hepatocyte growth factor/scatter factormet signalling in human cells concomitant with induction of the urokinase proteolysis network [Ｊ]. Mol Cell Biol， 1996; 16(3)∶1115.
6. Weidner KM, Di Cesare S, Sachs M, et al. Interaction between Gab1 and the cMet receptor tyrosine kinase is responsible for epithelial morphogenesis [Ｊ]. Nature， 1996; 384(6605)∶173.
7. Kosai K, Matsumoto K, Nagata S, et al. Abrogation of Fasinduced fulminant hepatic failure in mice by hepatocyte growth factor [Ｊ]. Biochem Biophys Res Commun， 1998; 244(3)∶683.
8. Huang TS, Lee SC, Lin JK. Suppression of cJun/AP1 activation by an inhibitor of tumor promotion in mouse fibroblast cells [Ｊ]. Proc Natl Acad Sci USA， 1991; 88(12)∶5292.
9. Samaha HS, Kelloff GJ, Steele V, et al. Modulation of apoptosis by sulindac, curcumin, phenylethyl3methylcaffeate, and 6phenylhexyl isothiocyanate: apoptotic index as a biomarker in colon cancer chemoprevention and promotion [Ｊ]. Cancer Res， 1997; 57(7)∶1301.
10. Huang MT, Lou YR, Xie JG, et al. Effect of dietary curcumin and dibenzoylmethane on formation of 7,12dimethylbenz [a] anthraceneinduced mammary tumors and lymphomas/leukemias in Sencar mice [Ｊ]. Carcinogenesis， 1998; 19(9)∶1697.
11. Chen H, Zhang ZS, Zhang YL, et al. Curcumin inhibits cell proliferation by interfering with the cell cycle and inducing apoptosis in colon carcinoma cells [Ｊ]. Anticancer Res， 1999; 19(5A)∶3675.
12. Moragoda L, Jaszewski R, Majumdar AP. Curcumin induced modulation of cell cycle and apoptosis in gastric and colon cancer cells [Ｊ]. Anticancer Res， 2001; 21(2A)∶873.
13. Ishiki Y, Ohnishi H, Muto Y, et al. Direct evidence that hepatocyte growth factor is a hepatotrophic factor for liver regeneration and has a potent antihepatitis effect in vivo [Ｊ]. Hepatology， 1992; 16(5)∶1227.
14. Xiao GH, Jeffers M, Bellacosa A, et al. Antiapoptotic signaling by hepatocyte growth factor/Met via the phosphatidylinositol 3kinase/Akt and mitogenactivated protein kinase pathways [Ｊ]. Proc Natl Acad Sci USA， 2001; 98(1)∶247.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Hepatocyte Growth Factor/Scatter Factor on Apoptosis of Colorectal Cancer Cells Induced with Curcumin

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