Objective To study whether carbon dioxide used to establish pneumoperitoneum has an influence on port-site and intraperitoneal implantation and metastasis of tumor cells.
Methods R15 hepatic cancer cells were injected into 30 Wistar rats’ peritoneal cavities 1 hour before operation, then the 30 Wistar rats were randomly divided into 3 groups: gasless group, helium group and carbon dioxide group. The suspension was exposed to the gas environment for 2 hours, all animals were killed after 28 days and the port-site and intraperitoneal implantation and metastasis of tumor cells were examined.
Results On port-site, intestinal serous coat, mesentery, greater omentum and diaphragm, the weights of tumor cells, in carbon dioxide group were (326.7±230.3) mg， （626.2±215.9） mg，（476.2±204.8） mg，（2 536.5±906.7） mg and （384.5±149.9） mg respectively； in helium group were (235.6±107.3) mg， （414.2±148.4） mg, （261.8±92.6） mg, （1 633.4±247.3） mg and（220.0±57.9） mg； in gasless group were (145.0±42.4) mg， （221.5±108.2） mg， （212.5±109.6） mg, （797.5±335.9） mg and 113.0 mg.The weights of carbon dioxide group showed a significant increase, compared with helium group and gasless group (P＜0.05). The weights of helium group were greater than gasless group，but there was no significance in statistics (P gt;0.05).
Conclusion The insufflation of carbon dioxide promotes intraperitoneal tumor implantation and growth compared with helium and gaslessness in a rat model.
Citation：
WANG Guangyi,MENG Xianying,GU Jianhua,Lv Guoyue. Effects of Pneumoperitoneum with Carbon Dioxide on Implantation and Growth of Tumor Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(2): 133-135. doi:
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- 2. Nduka CC, Monson JR, MenziesGow N, et al. Abdominal wall metastases following laparoscopy [Ｊ]. Br J Surg, 1994; 81(5)∶648.
- 3. Jacobi CA, Sabat R, Bohm B, et al. Pneumoperitoneum with carbon dioxide stimulates growth of malignant colonic cells [Ｊ]. Surgery, 1997; 121(1)∶72.
- 4. Bouvy ND, Marquet RL, Hamming JF, et al. Laparoscopic surgery in the rat. Beneficial effect on body weight and tumor take [Ｊ]. Surg Endosc, 1996; 10(5)∶490.
- 5. McMahon AJ, Baxter JN, Murray W, et al. Helium pneumoperitoneum for laparoscopic cholecystectomy：ventilatory and blood gas changes [Ｊ]. Br J Surg, 1994; 81(7)∶1033.
- 6. Bongard FS, Pianim NA, Leighton TA, et al. Helium insufflation for laparoscopic operation [Ｊ]. Surg Gynecol Obstet, 1993; 177(2)∶140.
- 7. Leighton TA, Liu SY, Bongard FS. Comparative cardiopulmonary effects of carbon dioxide versus helium pneumoperitoneum [Ｊ]. Surgery, 1993; 113(5)∶527.
