ObjectiveTo study the changes of lipopolysaccharide binding protein (LBP) in the serum of Wistar rats with obstructive jaundice and to investigate its potential mechanism.MethodsEighty male Wistar rats were randomly divided into obstructive jaundice group (OJ group, n=40） and sham operation group (SO group, n=40）. Before operation and the 5th, 10th, 15th, 20th day after common bile duct ligation, the levels of LBP, endotoxin, tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) in plasma were detected in all the rats. ResultsLBP levels in serum increased significantly in OJ group on the 10th day after operation compared with those of SO group. Moreover, LBP levels gradually increased in OJ group with the prolongation of obstructive time. A positive correlation existed between serum LBP and plasma endotoxin, TNF-α and IL-6.ConclusionThe study demonstrates that LBP in serum is high and plays an important role in the pathogenesis of multiple organ injury secondary to obstructive jaundice. It may be an appropriate way to treat patients with obstructive jaundice by decreasing LBP levels in serum.
Citation：
FANG Wencheng,KOU Zhimin,ZHANG Youcheng.. Change of Lipopolysaccharide Binding Protein and Its Significance in Rats with Obstructive Jaundice. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(4): 328-330. doi:
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- 2. Dawiskiba J. The role of endotoxaemia in the development of renal disorders in experimental obstructive jaundice in rats [Ｊ]. HPB Surg, 1996; 10(1)∶7.
- 3. 康焰，金晓东，吴淑红，等. 不同抗生素在G-细菌感染中诱导内毒素释放的研究 [Ｊ]. 中国普外基础与临床杂志， 2002； 9（1）∶13.
- 4. Opal SM, Scannon PJ, Vincent JL, et al. Relationship between plasma levels of lipopolysaccharide (LPS) and LPSbinding protein in patients with severe sepsis and septic shock [Ｊ]. J Infect Dis, 1999; 180(5)∶1584.
- 5. 姚咏明，于燕，方文慧，等. 创伤后内毒素增敏效应在多器官损害中的作用 [Ｊ]. 中国危重病急救医学， 1998； 10（12）∶712.
- 6. Schumann RR, Leong SR, Flaggs GW, et al. Structure and function of lipopolysaccharide binding protein [Ｊ]. Science, 1990; 249(4975)∶1429.
- 7. Iovine NM, Elsbach P, Weiss J. An opsonic function of the neutrophil bactericidal/permeabilityincreasing protein depends on both its Nand Cterminal domains [Ｊ]. Proc Natl Acad Sci USA, 1997; 94(20)∶10973.
- 8. 姚咏明，杨英祥，陆家齐，等. 脾切除对内毒素诱发大鼠急性肺损伤的影响及机制 [Ｊ]. 中华创伤杂志， 2001； 17（9）∶540.
