【Abstract】Objective To explore the distribution and migration of oval cells in progressive hepatic injury.
Methods Sixty SD rats were divided into the control group (n=20) and experimental group (n=40). After the establishment of hepatic carcinoma models, C-kit was continuously detected by immunohistochemistry and the liver pathologic changes were regularly observed by optical microscopy.
Results The hepatic surface was smooth with eumorphism in histology in the control group. The C-kit positive cells were occasionally found. In the experimental group, the oval cells with C-kit positive were initially discovered in the portal regions in the second week, and these cells proliferated along the bile duct epithelia. With the hepatic injury becoming more serious, the oval cells extended into the hepatic lobular regions from the portal regions. When hepatocellular carcinoma occurred，the majority were mixed carcinomas, and the oval cells were found inside and outside the carcinoma nodes. In this period, the most of C-kit positive cells still located in the portal regions.
Conclusion ①The oval cells are the most sensitive cells for the hepatic injury. ②The oval cells which migrate unruly participate in the formation of hepatic pseudolobules. ③The oval cells play an important role in hepatocarcinogenesis.
Citation：
GONG Jiaqing,FANG Chihua,LI Ya.. Experimental Research of Distribution and Migration of Oval Cells in Progressive Hepatic Injury. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(5): 389-392. doi:
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- 4. Baumann U, Crosby HA, Ramani P, et al. Expression of the stem cell factor receptor ckit in normal and diseased pediatric liver: identification of a human hepatic progenitor cell? [Ｊ]. Hepatology, 1999; 30(1)∶112.
- 5. 龚加庆，方驰华.大鼠卵圆细胞与原发性肝细胞癌发生的关系 [Ｊ]. 世界华人消化杂志， 2002； 10（10）∶1133.
- 6. Hellerbrand C, Jobin C, Licato LL, et al. Cytokines induce NFkappaB in activated but not in quiescent rat hepatic stellate cells [Ｊ]. Am J Physiol, 1998; 275(2 Pt 1)∶G269.
- 7. McCrudden R, Iredale JP. Liver fibrosis, the hepatic stellate cell and tissue inhibitors of metalloproteinases [Ｊ]. Histol Histopathol, 2000; 15(4)∶1159.
- 8. Gelderblom WC, Marasas WF, LebepeMazur S, et al. Interaction of fumonisin B(1) and aflatoxin B(1) in a shortterm carcinogenesis model in rat liver [Ｊ]. Toxicology, 2002; 171(2-3)∶161.
- 9. Ahn B, Han BS, Kim DJ, et al. Immunohistochemical localization of inducible nitric oxide synthase and 3nitrotyrosine in rat liver tumors induced by Nnitrosodiethylamine [Ｊ]. Carcinogenesis, 1999; 20(7)∶1337.
