Inducible Nitric Oxide Synthase and p53 Expression in Hepatocellular Carcinoma and Their Relationship with Angiogenesis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHANG Jian , HE Sheng , LI Maode.

Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China;

Corresponding author：

Keywords：

Hepatocellular carcinoma Inducible nitric oxide synthase p53 protein Angiogenesis

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【Abstract】Objective To investigate the expression of inducible nitric oxide synthase (iNOS) and p53 protein in hepatocellular carcinoma (HCC) and their relationship with angiogenesis.
Methods Immunohistochemical method and image analysis technique were used to detect the expression of iNOS and p53 protein in tumor tissue sections of 59 HCC patients. Microvessel density (MVD) was counted by immunohistochemical staining with anti-CD34 antibody.
Results ①The expression rates of iNOS and p53 were 81.4%(48/59), 64.4%(38/59) in HCC patients, respectively. The expression intensities of iNOS and p53 were 5 635±1 287, 3 352±873 in HCC patients, respectively. ②MVD was 32.5±2.73 in the tumor tissue of HCC patients. ③The expression of iNOS was correlated with the expression of p53 and MVD in HCC patients (P＜0.05); The expression of p53 was also correlated with the MVD in HCC patients (P＜0.05).
Conclusion iNOS and p53 are highly expressed in HCC and may play a key role in angiogenesis of HCC.

Citation： ZHANG Jian,HE Sheng,LI Maode.. Inducible Nitric Oxide Synthase and p53 Expression in Hepatocellular Carcinoma and Their Relationship with Angiogenesis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(5): 396-398. doi: Copy

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1. Papapetropoulos A, GarciaCardena G, Madri JA, et al. Nitric oxide production contributes to the angiogenic properties of vascular endothelial growth factor in human endothelial cells [Ｊ]. J Clin Invest, 1997; 100(12)∶3131.
2. Mukhopadhyay D, Tsiokas L, Sukhatme VP. Wildtype p53 and vSrc exert opposing influences on human vascular endothelial growth factor gene expression [Ｊ]. Cancer Res, 1995; 55(24)∶6161.
3. Mattern J, Koomagi R, Volm M. Biological characterization of subgroups of squamous cell lung carcinomas [Ｊ]. Clin Cancer Res, 1999; 5(6)∶1459.
4. Weidner N, Folkman J, Pozza F, et al. Tumor angiogenesis: a new significant and independent prognostic indicator in earlystage breast carcinoma [Ｊ]. J Natl Cancer Inst, 1992; 84(24)∶1875.
5. Rosbe KW, Prazma J, Petrusz P, et al. Immunohistochemical characterization of nitric oxide synthase activity in squamous cell carcinoma of the head and neck [Ｊ]. Otolaryngol Head Neck Surg, 1995; 113(5)∶541.
6. Thomsen LL, Lawton FG, Knowles RG, et al. Nitric oxide synthase activity in human gynecological cancer [Ｊ]. Cancer Res, 1994; 54(5)∶1352.
7. Fujimoto H, Ando Y, Yamashita T, et al. Nitric oxide synthase activity in human lung cancer [Ｊ]. Jpn J Cancer Res, 1997; 88(12)∶1190.
8. Parenti A, Morbidelli L, Cui XL, et al. Nitric oxide is an upstream signal of vascular endothelial growth factorinduced extracellular signalregulated kinase1/2 activation in postcapillary endothelium [Ｊ]. J Biol Chem, 1998; 273(7)∶4220.
9. Chin K, Kurashima Y, Ogura T, et al. Induction of vascular endothelial growth factor by nitric oxide in human glioblastoma and hepatocellular carcinoma cells [Ｊ]. Oncogene, 1997; 15(4)∶437.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Inducible Nitric Oxide Synthase and p53 Expression in Hepatocellular Carcinoma and Their Relationship with Angiogenesis

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