ObjectiveTo investigate the effects of somatostatin8 (SS8) on the apoptosis and the expression of cmyc protein of hepatocellular carcinoma cell SMMC7721. MethodsCultured in vitro, hepatocellular carcinoma cells SMMC7721 were incubated with SS8 (10 μg/ml). The apoptosis rate and expression of cmyc protein were detected by flow cytometry (FCM). ResultsSS8 can cause the spanonumber in S and G2/M phase and the auxonumber in G0/G1 phase of SMMC7721 cells . The apoptosis rate was 14.2% in the study group and 6.1% in the control group, and there was significant difference (P＜0.05); The level of expressions of cmyc protein was 0.833±0.035 after action by SS8 for 24 h. Compared with control group, there was no significant difference in the study group（P＞0.10）.But after the cells were incubated with SS8 for 48，72，96，120，144 h, the level of expressions of cmyc protein was 0.818±0.04，0.721±0.029，0.669±0.026，0.648±0.045，0.642±0.028 respectively in the study group, and there was significant difference as compared with the control group (P＜0.05). Conclusion The SS8 can induce the apoptosis and lower expression of cmyc protein of hepatocellular carcinoma cell SMMC7721.
Citation：
ZHANG Yanling,ZHANG Zheng. The Effects of Somatostatin Analog on the Apoptosis and the Expression of cmyc Protein of Hepatocellular Carcinoma Cell. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(1): 25-27. doi:
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- 2. Srikant CB.Cell cycle dependent induction of apoptosis by somatostatin analog SMS201995 in AtT20 mouse pituitary cells [Ｊ]. Biochem Biophys Res Commun，1995; 209(2)∶400.
- 3. Reubi JC. Clinical relevance of somatostatin receptor imaging [Ｊ]. Eur J Endocrinol，1994; 131(6)∶575.
- 4. Reubi JC, Laissue JA. Multiple actions of somatostatin in neoplastic disease [Ｊ]. Trends Pharmacol Sci，1995; 16(3)∶110.
- 5. Sharma K, Patel YC, Srikant CB. Subtypeselective induction of wildtype p53 and apoptosis, but not cell cycle arrest, by human somatostatin receptor 3 [Ｊ]. Mol Endocrinol，1996; 10(12)∶1688.
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- 7. Weinstein IB. The origins of human cancer: molecular mechanisms of carcinogenesis and their implications for cancer prevention and treatmenttwentyseventh G.H.A. Clowes memorial award lecture [Ｊ]. Cancer Res，1988; 48(15)∶4135.
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