Objective To investigate the mechanism of the resistance of pancreatic cancer cells to tumor necrosis factor related apoptosis inducing ligand (TRAIL)mediated apoptosis. MethodsThe expression of TRAIL receptor-4 (TRAIL-R4) in normal pancreas tissue and pancreatic cancer was analyzed by using Northern blotting, Western blotting and immunohistochemistry.ResultsTRAIL-R4 mRNA and protein were expressed at moderate to high levels in human pancreatic cancer, but demonstrated weak to negative in the normal pancreas. Moreover, pancreatic cancer cells showed b TRAIL-R4 immunostaining throughout the tumor mass. Conclusion TRAIL-R4 levels are significantly different in pancreatic cancer in comparison to the normal pancreas. These findings give new insights into the resistance mechanisms of pancreatic cancer cells towards TRAILmediated apoptosis.
Citation：
LIAO Quan,GUO Junchao,ZHAO Yupei. Expression of Tumor Necrosis Factor Related Apoptosis Inducing Ligand Receptor-4 in Human Pancreatic Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(6): 535-538. doi:
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- 1. Brammer R, Buckels J, Bramhall S. Advances in nonoperative therapy in pancreatic cancer [Ｊ]. Int J Clin Pract, 2000; 54(6)∶373.
- 2. Ozawa F, Friess H, Kleeff J, et al. Effects and expression of TRAIL and its apoptosispromoting receptors in human pancreatic cancer [Ｊ]. Cancer Lett, 2001; 163(1)∶71.
- 3. Walczak H, Miller RE, Ariail K, et al. Tumoricidal activity of tumor necrosis factorrelated apoptosisinducing ligand in vivo [Ｊ]. Nat Med, 1999; 5(2)∶157.
- 4. Pan G, Ni J, Yu GL, et al. TRUNDD, a new member of the TRAIL receptor family that antagonizes TRAIL signaling [Ｊ]. FEBS Letter, 1998; 424(1)∶41.
- 5. DegliEsposti MA, Dougall WC, Smolak PJ, et al. The novel receptor TRAILR4 induces NFκB and protects against TRAILmediated apoptosis, yet retains an incomplete death domain [Ｊ]. Immunity, 1997; 7(4)∶813.
- 6. Zhang XD, Franco AV, Nguyen T, et al. Differential localization and regulation of death and decoy receptors for TNFrelated apoptosisinducing ligand (TRAIL) in human melanoma cells [Ｊ]. J Immunol, 2000; 164(8)∶3961.
