Advances in Experimental Models of Chronic Pancreatitis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

CHEN Qilong ¹ , WEN Hao ² , LI Guowei ¹

Department of General Surgery, The Second Affiliated Hospital, Xi’an Jiaotong University, Xi’an 710004, China;

Corresponding author：

Keywords：

Chronic pancreatitis; Experimental model

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Objective To discuss the methods of producing experimental models of chronic pancreatitis and their individual properties. Methods The recent literatures about experimental models of chronic pancreatitis were reviewed and analyzed. Methods of producing experimental models and their individual properties were summarized, and best models suitable for varied chronic pancreatitis were afforded. Results Diet, ligation of pancreatic duct, caerulein, dibutyltin dichloride (DBTC), arterial ligation, injecting microspheres into artery, and injection of pancreatic duct could induce different experimental models of chronic pancreatitis. Spontaneous chronic pancreatitis was induced by diet, chronic obstructive pancreatitis produced by ligation and injection of pancreatic duct, chronic relapsing pancreatitis evoked by caerulein, and chronic active pancreatitis made by arterial ligation and injecting microspheres into artery.Conclusion Different methods could induce models of chronic pancreatitis, which had their individual properties.

Citation： CHEN Qilong,WEN Hao,LI Guowei. Advances in Experimental Models of Chronic Pancreatitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(6): 560-563. doi: Copy

1.	Etemad B, Whitcomb DC. Chronic pancreatitis: diagnosis, classification, and new genetic developments [J]. Gastroenterology, 2001; 120(3)∶682.
2.	佟卫刚，卢实春，胡骥，等. 慢性胰腺炎的外科治疗及远期疗效 [J]. 中国普外基础与临床杂志, 1999; 6(6)∶347.
3.	Ohashi K, Kim JH, Hara H, et al. WBN/Kob rats. A new spontaneously occurring model of chronic pancreatitis [J]. Int J pancreatol, 1990; 6(4)∶231.
4.	Su SB, Motoo Y, Xie MJ, et al. Expression of transforming growth factorbeta in spontaneous chronic pancreatitis in the WBN/Kob rat [J]. Dig Dis Sci, 2000; 45(1)∶151.
5.	耿智敏，向国安，韩庆，等. 转化生长因子β1在胆管愈合过程中的表达及意义 [J]. 中国普外基础与临床杂志, 2000; 7(6)∶362.
6.	Su SB, Motoo Y, Iovanna JL, et al. Effect of camostat mesilate on the expression of pancreatitisassociated protein (PAP), p8, and cytokines in rat spontaneous chronic pancreatitis [J]. Pancreas, 2001; 23(2)∶134.
7.	Su SB, Motoo Y, Xie MJ, et al. Apoptosis in rat spontaneous chronic pancreatis: role of the Fas and Fas ligand system [J]. Dig Dis Sci, 2001; 46(1)∶166.
8.	Widdison AL, Alvarez C, Schwarz M, et al. The influence of ethanol on pancreatic blood flow in cats with chronic pancreatitis [J]. Surgery, 1992; 112(2)∶202.
9.	Karanjia ND, Widdison AL, Leung F, et al. Compartment syndrome in experimental chronic obstructive pancreatitis: effect of decompressing the main pancreatic duct [J]. Br J Surg, 1994; 81(2)∶ 259.
10.	Reber PU, Patel AG, Toyama MT, et al. Feline model of chronic obstructive pancreatitis: effects of acute pancreatic duct decompression on blood flow and interstitial pH [J]. Scand J Gastroenterol, 1999; 34(4)∶439.
11.	Toyama MT, Patel AG, Nguyen T, et al. Effect of ethanol on pancreatic interstitial pH and blood flow in cats with chronic pancreatitis [J]. Ann Surg, 1997; 225(2)∶223.
12.	Patel AG, Reber PU, Toyama MT, et al. Effect of pancreaticojejunostomy on fibrosis, pancreatic blood flow, and interstitial pH in chronic pancreatitis: a feline model [J]. Ann Surg, 1999; 230(5)∶672.
13.	Reber PU, Patel AG, Lewis MP, et al. Stenting does not decompress the pancreatic duct as effective as surgery in experimental chronic pancreatitis [J]. Surgery, 1998; 124(3)∶561.
14.	李春艳，崔美芝，成晓松，等. 急性胰腺炎动物模型概况 [J]. 哈尔滨医科大学学报, 1998; 32(4)∶308.
15.	姜永锋，张延龄. 急性胰腺炎的实验研究进展 [J]. 国外医学外科学分册, 1996; 23(1)∶13.
16.	NeuschwanderTetri BA, Burton FR, Presti ME, et al. Repetitive selflimited acute pancreatitis induces pancreatic fibrogenesis in the mouse [J]. Dig Dis Sci, 2000; 45(4)∶665.
17.	Murayama KM, Barent BL, Gruber M, et al. Characterization of a novel model of pancreatic fibrosis and acinar atrophy [J]. J Gastrointest Surg, 1999; 3(4)∶418.
18.	Miyahara T, Kawabuchi M, Goto M, et al. Morphological study of pancreatic endocrine in an experimental chronic pancreatitis with diabetes induced by stress and cerulein [J]. Ultrastruct Pathol, 1999; 23(3)∶171.
19.	Merkord J, Weber H, Sparmann G, et al. The course of pancreatic fibrosis induced by dibutyltin dichloride (DBTC) [J]. Ann N Y Acad Sci, 1999; 880∶231.
20.	Merkord J, Weber H, Jonas L, et al. The influence of ethanol on longterm effects of dibutyltin dichloride (DBTC) in pancreas and liver of rats [J]. Hum Exp Toxicol, 1998; 17(3)∶144.
21.	Sparmann G, Merkord J, Jaschke A, et al. Pancreatic fibrosis in experimental pancreatitis induced by dibutyltin dichloride [J]. Gasteroenterology, 1997; 112(5)∶1664.
22.	Sparmann G, Behrend S, Merkord J, et al. Cytokine mRNA levels and lymphocyte infiltration in pancreatic tissue during experimental chronic pancreatitis induced by dibutyltin dichloride [J]. Dig Dis Sci, 2001; 46(8)∶1647.
23.	Freiburghaus AU, Redha F, Ammann RW. Does actue pancreatitis progress to chronic pancreatitis? A microvascular pancreatitis model in the rat [J]. Pancreas, 1995; 11(4)∶374.
24.	Tanaka T, Ichiba Y, Miura Y, et al. Canine model of chronic pancreatitis due to chronic ischemia [J]. Digestion, 1994; 55(2)∶86.
25.	Kataoka K, Sasaki T, Yorizumi H, et al. Pathophsiologic studies of experimental chronic pancreatitis in rats induced by injection of zeinoleic acidlinoleic acid solution into the pancreatic duct [J]. Pancreas, 1998; 16(3)∶289.
26.	Haber PS, Keogh GW, Apte MV, et al. Activation of pancreatic stellate cells in human and experimental pancreatic fibrosis [J]. Am J Pathol, 1999; 155(4)∶ 1087.
27.	梁林，冯凯琳，吕维富. 胰腺分裂在慢性胰腺炎病因学中的作用及其发病机制 [J]. 实用放射学杂志, 2000; 16(9)∶522.
28.	Vogelmann R, Ruf D, Wagner M, et al. Effects of fibrogenic mediators on the development of pancreatic fibrosis in a TGFbeta1 transgenic mouse model [J]. Am J Physiol Gastrointest Liver Physiol, 2001; 280(1)∶ G164.

1. Etemad B, Whitcomb DC. Chronic pancreatitis: diagnosis, classification, and new genetic developments [J]. Gastroenterology, 2001; 120(3)∶682.
2. 佟卫刚，卢实春，胡骥，等. 慢性胰腺炎的外科治疗及远期疗效 [J]. 中国普外基础与临床杂志, 1999; 6(6)∶347.
3. Ohashi K, Kim JH, Hara H, et al. WBN/Kob rats. A new spontaneously occurring model of chronic pancreatitis [J]. Int J pancreatol, 1990; 6(4)∶231.
4. Su SB, Motoo Y, Xie MJ, et al. Expression of transforming growth factorbeta in spontaneous chronic pancreatitis in the WBN/Kob rat [J]. Dig Dis Sci, 2000; 45(1)∶151.
5. 耿智敏，向国安，韩庆，等. 转化生长因子β1在胆管愈合过程中的表达及意义 [J]. 中国普外基础与临床杂志, 2000; 7(6)∶362.
6. Su SB, Motoo Y, Iovanna JL, et al. Effect of camostat mesilate on the expression of pancreatitisassociated protein (PAP), p8, and cytokines in rat spontaneous chronic pancreatitis [J]. Pancreas, 2001; 23(2)∶134.
7. Su SB, Motoo Y, Xie MJ, et al. Apoptosis in rat spontaneous chronic pancreatis: role of the Fas and Fas ligand system [J]. Dig Dis Sci, 2001; 46(1)∶166.
8. Widdison AL, Alvarez C, Schwarz M, et al. The influence of ethanol on pancreatic blood flow in cats with chronic pancreatitis [J]. Surgery, 1992; 112(2)∶202.
9. Karanjia ND, Widdison AL, Leung F, et al. Compartment syndrome in experimental chronic obstructive pancreatitis: effect of decompressing the main pancreatic duct [J]. Br J Surg, 1994; 81(2)∶ 259.
10. Reber PU, Patel AG, Toyama MT, et al. Feline model of chronic obstructive pancreatitis: effects of acute pancreatic duct decompression on blood flow and interstitial pH [J]. Scand J Gastroenterol, 1999; 34(4)∶439.
11. Toyama MT, Patel AG, Nguyen T, et al. Effect of ethanol on pancreatic interstitial pH and blood flow in cats with chronic pancreatitis [J]. Ann Surg, 1997; 225(2)∶223.
12. Patel AG, Reber PU, Toyama MT, et al. Effect of pancreaticojejunostomy on fibrosis, pancreatic blood flow, and interstitial pH in chronic pancreatitis: a feline model [J]. Ann Surg, 1999; 230(5)∶672.
13. Reber PU, Patel AG, Lewis MP, et al. Stenting does not decompress the pancreatic duct as effective as surgery in experimental chronic pancreatitis [J]. Surgery, 1998; 124(3)∶561.
14. 李春艳，崔美芝，成晓松，等. 急性胰腺炎动物模型概况 [J]. 哈尔滨医科大学学报, 1998; 32(4)∶308.
15. 姜永锋，张延龄. 急性胰腺炎的实验研究进展 [J]. 国外医学外科学分册, 1996; 23(1)∶13.
16. NeuschwanderTetri BA, Burton FR, Presti ME, et al. Repetitive selflimited acute pancreatitis induces pancreatic fibrogenesis in the mouse [J]. Dig Dis Sci, 2000; 45(4)∶665.
17. Murayama KM, Barent BL, Gruber M, et al. Characterization of a novel model of pancreatic fibrosis and acinar atrophy [J]. J Gastrointest Surg, 1999; 3(4)∶418.
18. Miyahara T, Kawabuchi M, Goto M, et al. Morphological study of pancreatic endocrine in an experimental chronic pancreatitis with diabetes induced by stress and cerulein [J]. Ultrastruct Pathol, 1999; 23(3)∶171.
19. Merkord J, Weber H, Sparmann G, et al. The course of pancreatic fibrosis induced by dibutyltin dichloride (DBTC) [J]. Ann N Y Acad Sci, 1999; 880∶231.
20. Merkord J, Weber H, Jonas L, et al. The influence of ethanol on longterm effects of dibutyltin dichloride (DBTC) in pancreas and liver of rats [J]. Hum Exp Toxicol, 1998; 17(3)∶144.
21. Sparmann G, Merkord J, Jaschke A, et al. Pancreatic fibrosis in experimental pancreatitis induced by dibutyltin dichloride [J]. Gasteroenterology, 1997; 112(5)∶1664.
22. Sparmann G, Behrend S, Merkord J, et al. Cytokine mRNA levels and lymphocyte infiltration in pancreatic tissue during experimental chronic pancreatitis induced by dibutyltin dichloride [J]. Dig Dis Sci, 2001; 46(8)∶1647.
23. Freiburghaus AU, Redha F, Ammann RW. Does actue pancreatitis progress to chronic pancreatitis? A microvascular pancreatitis model in the rat [J]. Pancreas, 1995; 11(4)∶374.
24. Tanaka T, Ichiba Y, Miura Y, et al. Canine model of chronic pancreatitis due to chronic ischemia [J]. Digestion, 1994; 55(2)∶86.
25. Kataoka K, Sasaki T, Yorizumi H, et al. Pathophsiologic studies of experimental chronic pancreatitis in rats induced by injection of zeinoleic acidlinoleic acid solution into the pancreatic duct [J]. Pancreas, 1998; 16(3)∶289.
26. Haber PS, Keogh GW, Apte MV, et al. Activation of pancreatic stellate cells in human and experimental pancreatic fibrosis [J]. Am J Pathol, 1999; 155(4)∶ 1087.
27. 梁林，冯凯琳，吕维富. 胰腺分裂在慢性胰腺炎病因学中的作用及其发病机制 [J]. 实用放射学杂志, 2000; 16(9)∶522.
28. Vogelmann R, Ruf D, Wagner M, et al. Effects of fibrogenic mediators on the development of pancreatic fibrosis in a TGFbeta1 transgenic mouse model [J]. Am J Physiol Gastrointest Liver Physiol, 2001; 280(1)∶ G164.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Advances in Experimental Models of Chronic Pancreatitis

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