Studies on the Expression of Survivin Protein and Its Relationship to the Proliferation of the Tumor Cells and Prognosis in Primary Hepatocellular Carcinoma_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

TIAN Fuzhou ¹ , CHEN Yuqiong ² , CHEN Tao ¹ , YIN Zhiliang ¹

Department of General Surgery, Chengdu Army General Hospital, Chendu 610083, China;

Corresponding author：

Keywords：

Hepatocellular carcinoma; Proliferating cell nuclear antigen; survivin protein

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

ObjectiveTo study the expression of survivin protein in primary hepatocellular carcinoma(PHC) and its relationship to the proliferation of the tumor cells and prognosis of PHC. MethodsThe expression of survivin protein and the proliferation of tumor cells marked by proliferating cell nuclear antigen (PCNA) in 48 cases of PHC were determined by immunohistochemical method. ResultsThe survivin protein was expressed in 31 of 48 cases of PHC (64.6%). The expression of PCNA was significantly higher in hepatocellular carcinoma (HCC) with positive survivin expression than in HCC with negative survivin expression. The patients with positive survivin expression had the worse prognosis than those with negative survivin expression. ConclusionThe expression of survivin may play an important role in the proliferation of PHC cells and closely associate with the prognosis of PHC, and probably become the prognostic factor and an important target of therapy.

Citation： TIAN Fuzhou,CHEN Yuqiong,CHEN Tao,YIN Zhiliang. Studies on the Expression of Survivin Protein and Its Relationship to the Proliferation of the Tumor Cells and Prognosis in Primary Hepatocellular Carcinoma. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(2): 131-133. doi: Copy

1.	李连第，张思维，鲁凤珠，等. 中国恶性肿瘤死亡谱及分类构成研究 [J]. 中华肿瘤杂志， 1997； 19（5）∶323.
2.	GraslKraupp B, RuttkayNedecky B, Mullauer L, et al. Inherent increase of apoptosis in liver tumors: implications for carcinogenesis and tumor regression [J]. Hepatology， 1997； 25(4)∶906.
3.	Terada T, Nakanuma Y. Expression of apoptosis, proliferation cell nuclear antigen, and apoptosisrelated antigen (bcl2, cmyc, Fas, Lewisy, and p53) in human cholangiocarcinomas and hepatocellular carcinomas [J]. Pathol Int，1996； 46(10)∶764.
4.	Farber E. Hepatocyte proliferation in stepwise development of experimental liver cell cancer [J]. Dig Dis Sci, 1991； 36（7）∶973.
5.	Edmondson HA,Steiner PE. Primary carcinoma of liver. A study of 100 cases among 48 900 necropsies [J]. Cancer, 1954； 7∶462.
6.	Staunton MJ, Gaffney EF. Tumor type is a determinant of susceptibility to apoptosis [J]. Am J Clin Pathol, 1995； 103(3)∶300.
7.	Soini Y, Virkajarvi N, Lehto VP, et al. Hepatocellular carcinomas with a high proliferation index and a low degree of apoptosis and necrosis are associated with a shortened survival [J]. Br J Cancer, 1996； 73（9）∶1025.
8.	Bravo R, Frank R, Blundell PA, et al.Cyclin/PCNA is the auxiliary protein of DNA polymerasedelta [J]. Nature, 1987； 326(6112)∶515.
9.	阮幼冰，武忠弼，杨木兰，等. 大鼠肝癌发生过程中增殖细胞核抗原及表达 [J]. 中华病理学杂志，1996； 25（1）∶39.
10.	Hino N, Higashi T, Nouso K, et al. Proliferating cell nuclear antigen and grade of malignancy in small hepatocellular carcinomaevaluation in USguided specimens [J]. Hepatogastroenterology, 1997； 44(13)∶245.
11.	Adida C, Crotty PL, McGrath J, et al. Developmentally regulated expression of the novel cancer antiapoptosis gene survivin in human and mouse differentiation [J]. Am J Pathol,1998； 152(1)∶43.
12.	Ambrosini G, Adida C, Altieri DC. A novel antiapoptosis gene, survivin, expressed in cancer and lymphoma [J]. Nat Med, 1997； 3(8)∶917.
13.	Tamm I, Wang Y, Sausville E, et al.IAPfamily protein survivin inhibits caspase activity and apoptosis induced by Fas(CD95), Bax, caspases, and anticancer drugs [J]. Cancer Res, 1998； 58(23)∶5315.
14.	Sarela AI, Macadam RC, Farmery SM, et al. Expression of the antiapoptosis gene, survivin, predicts death from recurrent colorectal carcinoma [J]. Gut, 2000； 46(5)∶645.
15.	Monzo M, Rosell R, Felip E, et al. A novel antiapoptosis gene: Reexpression of survivin messenger RNA as a prognosis marker in nonsmallcell lung cancer [J]. J Clin Oncol, 1999； 17(7)∶2100.
16.	Islam A, Kageyama H, Takada N, et al. High expression of survivin, mapped to 17q25, is significantly associated with poor prognostic factors and promotes cell survival in human neuroblastoma [J]. Oncogene, 2000； 19(5)∶617.
17.	Suzuki A, Ito T, Kawano H, et al. Survivin initiates procaspase 3/p21 complex formation as a result of interaction with CDK4 to resist Fasmediated cell death [J]. Oncogene, 2000； 19(10)∶1346.
18.	Ishida M, Gomyo Y, Tatebe S, et al. Apoptosis in human gastric mucosa, chronic gastritis, dysplasia and carcinoma: analysis by terminal deoxynucleotidyl transferasemediated dUTPbiotin nick end labelling [J]. Virchows Arch, 1996； 428(4-5)∶229.
19.	Shin S, Sung BJ, Cho YS, et al. An antiapoptotic protein human survivin is a direct inhibitor of caspase3 and 7 [J]. Biochemistry, 2001; 40(4)∶1117.
20.	Ito Y, Matsuura N, Sakon M, et al. Both cell proliferation and apoptosis significantly predict shortened diseasefree survival in hepatocellular carcinoma [J]. Br J Cancer, 1999； 81(4)∶747.

1. 李连第，张思维，鲁凤珠，等. 中国恶性肿瘤死亡谱及分类构成研究 [J]. 中华肿瘤杂志， 1997； 19（5）∶323.
2. GraslKraupp B, RuttkayNedecky B, Mullauer L, et al. Inherent increase of apoptosis in liver tumors: implications for carcinogenesis and tumor regression [J]. Hepatology， 1997； 25(4)∶906.
3. Terada T, Nakanuma Y. Expression of apoptosis, proliferation cell nuclear antigen, and apoptosisrelated antigen (bcl2, cmyc, Fas, Lewisy, and p53) in human cholangiocarcinomas and hepatocellular carcinomas [J]. Pathol Int，1996； 46(10)∶764.
4. Farber E. Hepatocyte proliferation in stepwise development of experimental liver cell cancer [J]. Dig Dis Sci, 1991； 36（7）∶973.
5. Edmondson HA,Steiner PE. Primary carcinoma of liver. A study of 100 cases among 48 900 necropsies [J]. Cancer, 1954； 7∶462.
6. Staunton MJ, Gaffney EF. Tumor type is a determinant of susceptibility to apoptosis [J]. Am J Clin Pathol, 1995； 103(3)∶300.
7. Soini Y, Virkajarvi N, Lehto VP, et al. Hepatocellular carcinomas with a high proliferation index and a low degree of apoptosis and necrosis are associated with a shortened survival [J]. Br J Cancer, 1996； 73（9）∶1025.
8. Bravo R, Frank R, Blundell PA, et al.Cyclin/PCNA is the auxiliary protein of DNA polymerasedelta [J]. Nature, 1987； 326(6112)∶515.
9. 阮幼冰，武忠弼，杨木兰，等. 大鼠肝癌发生过程中增殖细胞核抗原及表达 [J]. 中华病理学杂志，1996； 25（1）∶39.
10. Hino N, Higashi T, Nouso K, et al. Proliferating cell nuclear antigen and grade of malignancy in small hepatocellular carcinomaevaluation in USguided specimens [J]. Hepatogastroenterology, 1997； 44(13)∶245.
11. Adida C, Crotty PL, McGrath J, et al. Developmentally regulated expression of the novel cancer antiapoptosis gene survivin in human and mouse differentiation [J]. Am J Pathol,1998； 152(1)∶43.
12. Ambrosini G, Adida C, Altieri DC. A novel antiapoptosis gene, survivin, expressed in cancer and lymphoma [J]. Nat Med, 1997； 3(8)∶917.
13. Tamm I, Wang Y, Sausville E, et al.IAPfamily protein survivin inhibits caspase activity and apoptosis induced by Fas(CD95), Bax, caspases, and anticancer drugs [J]. Cancer Res, 1998； 58(23)∶5315.
14. Sarela AI, Macadam RC, Farmery SM, et al. Expression of the antiapoptosis gene, survivin, predicts death from recurrent colorectal carcinoma [J]. Gut, 2000； 46(5)∶645.
15. Monzo M, Rosell R, Felip E, et al. A novel antiapoptosis gene: Reexpression of survivin messenger RNA as a prognosis marker in nonsmallcell lung cancer [J]. J Clin Oncol, 1999； 17(7)∶2100.
16. Islam A, Kageyama H, Takada N, et al. High expression of survivin, mapped to 17q25, is significantly associated with poor prognostic factors and promotes cell survival in human neuroblastoma [J]. Oncogene, 2000； 19(5)∶617.
17. Suzuki A, Ito T, Kawano H, et al. Survivin initiates procaspase 3/p21 complex formation as a result of interaction with CDK4 to resist Fasmediated cell death [J]. Oncogene, 2000； 19(10)∶1346.
18. Ishida M, Gomyo Y, Tatebe S, et al. Apoptosis in human gastric mucosa, chronic gastritis, dysplasia and carcinoma: analysis by terminal deoxynucleotidyl transferasemediated dUTPbiotin nick end labelling [J]. Virchows Arch, 1996； 428(4-5)∶229.
19. Shin S, Sung BJ, Cho YS, et al. An antiapoptotic protein human survivin is a direct inhibitor of caspase3 and 7 [J]. Biochemistry, 2001; 40(4)∶1117.
20. Ito Y, Matsuura N, Sakon M, et al. Both cell proliferation and apoptosis significantly predict shortened diseasefree survival in hepatocellular carcinoma [J]. Br J Cancer, 1999； 81(4)∶747.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Studies on the Expression of Survivin Protein and Its Relationship to the Proliferation of the Tumor Cells and Prognosis in Primary Hepatocellular Carcinoma

Abstract Full text Figures/Tables Video References Cited by

Format

Content