ObjectiveTo study the relationship between expression of p27KIP1 and progression of hepatocellular carcinoma(HCC).MethodsThe expression of p27KIP1 in 52 cases of HCC was detected by immunohistochemistry of strept avidinbiotin complex and mRNA in situ hybridization.ResultsThe positive cells of p27KIP1 protein were diffused in HCC.The positive signal was localized in nuclei.The labeling index (LI) of p27KIP1 protein was significantly higher in tumorsurrounding tissues than that in tumor tissues. p27KIP1 protein LI showed a positive correlation with the differentiation grade of HCC.The better differentiation of cancer cells, the higher LI of p27KIP1 protein (P＜0.01).The positive cells of p27KIP1 mRNA were also diffused in HCC.The positive signal was localized in nuclei and cytoplasm. As to the expression of p27KIP1 at the mRNA level,there was no significant correlation with tumorsurrounding tissues and stages of HCC.Conclusionp27KIP1 protein is associated with progression and differentiation grade of hepatocellular carcinoma.
Citation：
ZHENG Jianyong,LI Kaizong,WANG Weizhong,LI Jiang. Study on p27KIP1 Expression in Hepatocellular Carcinoma. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(3): 240-242. doi:
Copy
Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
| 1. |
Lloyd RV, Erickson LA, Jin L, et al. p27KIP1, a multifunctional cyclindependent kinase inhibitor with prognostic significance in human cancers [J]. Am J Pathol, 1999; 154(2)∶ 313.
|
| 2. |
Peng D, Fan Z, Lu Y, et al. Antiepidexmal growth factor receptor monoclonal antibody 225 upregulates p27KIP1and induces G1 arrest in prostatic cancer cell line DU1451 [J]. Cancer Res, 1996; 56（16）∶ 3666.
|
| 3. |
Polyak K, Kato JY, Solomon MJ, et al. p27KIP1, a cyclinCDK inhibitor; links transforming growth factorbeta and contact inhibition to cell cycle arrest [J]. Genes Dey, 1994; 8（1）∶ 9.
|
| 4. |
郭贵龙，姚榛祥. p27在甲状腺滤泡状肿瘤中的表达及其意义 [J]. 中国普外基础与临床杂志， 2001； 8（5）∶ 324.
|
| 5. |
夏加增，杨继昌，张熔熔,等. 胃癌中cyclin D1、cyclin E和p27的表达及其意义 [J]. 中国普外基础与临床杂志， 2001； 8（3）∶ 148.
|
| 6. |
Tsihlias J, Kapusta LR, DeBoer G, et al. Loss of cyclindependent kinase inhibitor p27KIP1 is a novel prognostic factor in localized human prostate adenocarcinoma [J]. Cancer Res, 1998; 58（3）∶ 542.
|
| 7. |
Kawamata N, Morosetti R, Miller CW, et al. Molecular analysis of the cyclindependent kinase inhibitor gene p27/Kip1 in human malignancies [J]. Cancer Res, 1995; 55（11）∶ 2266.
|
| 8. |
Zhang Y, Lin SC. Molecular characterization of the cyclindependent kinase inhibitor p27 promoter [J]. Biochim Biophys Acta, 1997; 1353(3)∶ 307.
|
| 9. |
Loda M, Cukor B, Tam SW, et al. Increased proteasomedependent degradation of the cyclindependent kinase inhibitor p27 in aggressive colorectal carcinomas [J]. Nat Med, 1997; 3（2）∶ 231.
|
- 1. Lloyd RV, Erickson LA, Jin L, et al. p27KIP1, a multifunctional cyclindependent kinase inhibitor with prognostic significance in human cancers [J]. Am J Pathol, 1999; 154(2)∶ 313.
- 2. Peng D, Fan Z, Lu Y, et al. Antiepidexmal growth factor receptor monoclonal antibody 225 upregulates p27KIP1and induces G1 arrest in prostatic cancer cell line DU1451 [J]. Cancer Res, 1996; 56（16）∶ 3666.
- 3. Polyak K, Kato JY, Solomon MJ, et al. p27KIP1, a cyclinCDK inhibitor; links transforming growth factorbeta and contact inhibition to cell cycle arrest [J]. Genes Dey, 1994; 8（1）∶ 9.
- 4. 郭贵龙，姚榛祥. p27在甲状腺滤泡状肿瘤中的表达及其意义 [J]. 中国普外基础与临床杂志， 2001； 8（5）∶ 324.
- 5. 夏加增，杨继昌，张熔熔,等. 胃癌中cyclin D1、cyclin E和p27的表达及其意义 [J]. 中国普外基础与临床杂志， 2001； 8（3）∶ 148.
- 6. Tsihlias J, Kapusta LR, DeBoer G, et al. Loss of cyclindependent kinase inhibitor p27KIP1 is a novel prognostic factor in localized human prostate adenocarcinoma [J]. Cancer Res, 1998; 58（3）∶ 542.
- 7. Kawamata N, Morosetti R, Miller CW, et al. Molecular analysis of the cyclindependent kinase inhibitor gene p27/Kip1 in human malignancies [J]. Cancer Res, 1995; 55（11）∶ 2266.
- 8. Zhang Y, Lin SC. Molecular characterization of the cyclindependent kinase inhibitor p27 promoter [J]. Biochim Biophys Acta, 1997; 1353(3)∶ 307.
- 9. Loda M, Cukor B, Tam SW, et al. Increased proteasomedependent degradation of the cyclindependent kinase inhibitor p27 in aggressive colorectal carcinomas [J]. Nat Med, 1997; 3（2）∶ 231.
