Change of Nuclear Factor Kappa B Activity in Patients with Acute Cholangitis of Severe Type and Its Clinical Significance_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LIU Changan , GONG Jianping , WU Chuanxin , LI Shengwei , LI Yue , YANG Kang , SHI Yujun , LI Xuhong

Department of General Surgery, The Second Affiliated Hospital, Chongqing University of Medical Science, Chongqing 400010, China;

Corresponding author：

Keywords：

Acute cholangitis of severe typePeripheral blood mononuclear cellNuclear factor kappa BCytokines

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ObjectiveTo determine the nuclear factor kappa B (NFkB) activity in peripheral blood mononuclear cells (PBMC) in patients with acute cholangitis of severe type (ACST) and correlate the degree of NFkB activation with severity of biliary tract infection and clinical outcome.MethodsTwenty patients with ACST were divided into survivor group (14 cases) and nonsurvivor group (6 cases). Other 10 patients undergoing elective gastrectomy or inguinal hernia repair were selected as control group. Peripheral blood samples were taken 24 hours after operation, PBMC was separated and nuclear proteins were isolated from PBMC, and NFkB was determined with electrophoretic mobility shift assay (EMSA). The levels of TNFα, IL6 and IL10 in plasma were determined by using an enzymelinked immunoassay (ELISA). ResultsThe NFkB activity was 5.02±1.03, 2.98±0.51 and 1.02±0.34 respectively in three groups. It was increased in all patients with ACST, versus the control group (P＜0.05), and the patients of nonsurvivor group had higher levels of NFkB activation than those of survivor group (P＜0.05). The levels of TNFα and IL6 were (496.28±52.35) ng/L and (578.13±67.72) ng/L in nonsurvivor group; (284.47±39.41) ng/L and (318.67±34.92) ng/L in survivor group; (89.43±10.39) ng/L and (101.27±13.47) ng/L in control group. All patients with ACST had increased levels of TNFα and IL6, which were many fold greater than that of control group, and there was an evidence of significantly higher levels in nonsurvivor group than in survivor group (P＜0.05). All patients had also increased levels of IL10 as compared to control group (P＜0.05), but the IL10 concentrations in plasma were not significantly higher in nonsurvivors than that of in those survivors (P gt;0.05). ConclusionNFkB activation in PBMCs in patients with ACST

Citation： LIU Changan,GONG Jianping,WU Chuanxin,LI Shengwei,LI Yue,YANG Kang,SHI Yujun,LI Xuhong. Change of Nuclear Factor Kappa B Activity in Patients with Acute Cholangitis of Severe Type and Its Clinical Significance. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(5): 445-447. doi: Copy

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1. 黄志强.黄志强胆道外科 [M]. 济南：山东科学技术出版社,.
2. Huang ZQ. New development of biliary surgery in China [J]. World J Gastroenterol, 2000; 6(2)∶187.
3. 黄显凯，韩本立. 急性胆管炎多形核白细胞聚集及其肝损害作用的研究 [J]. 中国普外基础与临床杂志, 1999; 6(3)∶146.
4. 黄显凯，韩本立. 急性胆管炎时肝微循环的变化及其细胞间粘附分子1和E选择素的作用 [J]. 中国普外基础与临床杂志, 1999; 6(5)∶270.
5. Christman JW, Lancaster LH, Blackwell TS. Nuclear factorkB: a pivotal role in the systemic inflammatory response syndrome and new target for therapy [J]. Intensive Care Med, 1998; 24(11)∶1131.
6. Arnalich F, GarciaPlamero E, Lopez J, et al. Predictive value of nuclear factor kappa B activity and plasma cytokine levels in patients with sepsis [J]. Infect Immun, 2000； 68(4)∶1942.
7. Foulds S, Galustian C, Mansfield AO, et al. Transcription factor NFkB expression and postsurgical organ dysfunction [J]. Ann Surg, 2001; 233(1)∶70.
8. Pennington C, Dunn J, Li C, et al. Nuclear factor kB activation in acute appendicitis: a molecular marker for extent of disease? [J]. Am Surg, 2000; 66(10)∶914.
9. Gong JP, Liu CA, Wu CX, et al. Nuclear factor kB activity in patients with acute severe cholangitis [J]. World J Gastroenterol, 2002; 8(2)∶346.
10. 龚建平，韩本立. 急性胆道感染所致多脏器损害发病机理的研究进展 [J]. 中国普外基础与临床杂志, 2002； 9(2)∶133.
11. AdibConquy M, Adrie C, Moine P, et al. NFkB expression in mononuclear cells of patients with sepsis resembles that observed in lipopolysaccharide tolerance [J]. Am J Respir Crit Care Med, 2000; 162(5)∶1877.
12. Gong JP, Wu CX, Liu CA, et al. Liver sinusoidal endothelial cell injury by neutrophils in rats with acute obstructive cholangitis [J]. World J Gastroenterol, 2002; 8(2)∶342.
13. Bohrer H, Qiu F, Zimmermann T, et al. Role of NF kappa B in the mortality of sepsis [J]. J Clin Invest, 1997; 100(5)∶972.
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15. 罗艳，梁华平，胡承香，等. 创伤小鼠核转录因子AP1活性及IL2表达变化 [J]. 第三军医大学学报， 2000； 22（11）∶1019.
16. ∶200～232.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Change of Nuclear Factor Kappa B Activity in Patients with Acute Cholangitis of Severe Type and Its Clinical Significance

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