ObjectiveTo investigate the expression and distribution of CD15s antigen in breast cancer and its relationship with carcinogenesis, progression and metastatic proclivity. MethodsCatalyzed signal amplification(CSA) immunohistochemical technique was used to detect the expression of CD15s antigen in breast cancer and in adjacent normal mucosa. Immunoelectromicroscopic ultrastructural localization of CD15s antigen labelled by colloidal gold was also bserved.ResultsThe positive rate of CD15s antigen expression in primary breast cancer was 79.8%(75/94). In adjacent normal mucosa (n=10) CD15s antigen showed weaker staining. The positive rate of CD15s antigen expression in grade Ⅱ-Ⅲ (87.3%) was notably higher than that in grade Ⅰ (69.2%, P＜0.05). In patients with lymph node metastasis, the positive rate of CD15s antigen expression was 90.2%, which was significantly higher than 67.4% in nodes with no metastasis (P＜0.05). CD15s antigen immunoreactivity was mainly localized in the border membrane of cytoplasm, endoplasmic reticulum, golgi complex and surrounding nuclear membrane in tumor tissue, and in the border membrane of cytoplasm in adjacent normal tissue. Conclusion CD15s antigen is a practical parameter for evaluating the degree of malignancy and lymphatic metastatic proclivity of breast cancer. It can provide a new pathway to investigate the carcinogenesis and progression of breast cancer.
Citation：
GU Huaping,SHANG Peizhong,NI Canrong.. Expression of CD15s Antigen in Breast Cancer and Its Clinical Significance. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2002, 9(5): 313-315. doi:
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| 1. |
李树玲. 中国常见恶性肿瘤诊治规范（乳腺癌） [M]. 北京： 北京医科大学、中国协和医科大学联合出版社，1990∶59～60.
|
| 2. |
Xing Y, Zao FK, Zhang SM, et al. Relationship between CD44v6 expression and prognosis in gastric carcinoma patients [J]. Shijie Huaren Xiaohua ZaZhi, 1999; 7(3)∶210.
|
| 3. |
Torii A, Nakayama A, Harada A, et al. Expression of the CD15 antigen in hepatocellular carcinoma [J]. Cancer, 1993; 71(12)∶3864.
|
| 4. |
尚培中，谷化平，孙印臣. 唾液酸化Lewis X在大肠癌中的表达及其意义 [J]. 中国普外基础与临床杂志， 2001； 8（3）∶151.
|
| 5. |
Yamaguchi A, Goi T, Seki K, et al. Chinical significance of combined immunohistochemical detection of CD44v and sialyl LeX expression for colorectal cancer patients undergoing curative resection [J]. Oncology, 1998; 55(5)∶400.
|
| 6. |
谷化平，尚培中，苏红，等. CD15抗原和bcl2基因蛋白在甲状腺癌中的表达及相关性研究 [J]. 中国普外基础与临床杂志， 2000； 7（2）∶ 97.
|
| 7. |
谷化平，倪灿荣，詹溶洲，等. 肝细胞癌中CD15和nm23H1的mRNA表达及其意义 [J]. 中国普外基础与临床杂志， 2001； 8（4）∶ 242.
|
| 8. |
Bresalier RS, Ho SB, Schoeppner HL, et al. Enhanced sialylation of mucinassociated carbohydrate structures in human colon cancer metastasis [J]. Gastroenterology, 1996; 110(5)∶1354.
|
| 9. |
唐建武. 凝集素受体表达与肿瘤 [J]. 国外医学分子生物学分册，1993； 15（1）∶ 34.
|
- 1. 李树玲. 中国常见恶性肿瘤诊治规范（乳腺癌） [M]. 北京： 北京医科大学、中国协和医科大学联合出版社，1990∶59～60.
- 2. Xing Y, Zao FK, Zhang SM, et al. Relationship between CD44v6 expression and prognosis in gastric carcinoma patients [J]. Shijie Huaren Xiaohua ZaZhi, 1999; 7(3)∶210.
- 3. Torii A, Nakayama A, Harada A, et al. Expression of the CD15 antigen in hepatocellular carcinoma [J]. Cancer, 1993; 71(12)∶3864.
- 4. 尚培中，谷化平，孙印臣. 唾液酸化Lewis X在大肠癌中的表达及其意义 [J]. 中国普外基础与临床杂志， 2001； 8（3）∶151.
- 5. Yamaguchi A, Goi T, Seki K, et al. Chinical significance of combined immunohistochemical detection of CD44v and sialyl LeX expression for colorectal cancer patients undergoing curative resection [J]. Oncology, 1998; 55(5)∶400.
- 6. 谷化平，尚培中，苏红，等. CD15抗原和bcl2基因蛋白在甲状腺癌中的表达及相关性研究 [J]. 中国普外基础与临床杂志， 2000； 7（2）∶ 97.
- 7. 谷化平，倪灿荣，詹溶洲，等. 肝细胞癌中CD15和nm23H1的mRNA表达及其意义 [J]. 中国普外基础与临床杂志， 2001； 8（4）∶ 242.
- 8. Bresalier RS, Ho SB, Schoeppner HL, et al. Enhanced sialylation of mucinassociated carbohydrate structures in human colon cancer metastasis [J]. Gastroenterology, 1996; 110(5)∶1354.
- 9. 唐建武. 凝集素受体表达与肿瘤 [J]. 国外医学分子生物学分册，1993； 15（1）∶ 34.
