【Abstract】Objective To investigate the inhibitory effects of flavonoids quercetin on the occurrence and proliferation of experimental mammary carcinoma. Methods DMBA induced mammary carcinoma was produced in rats. Seventy-nine female Sprague-Dawly rats were divided randomly into four groups: DMBA, DMBA with TAM, DMBA with quercetin and control. Chemicals had been administered to group A, group B, group C and group D respectively for 28 weeks. Samples of breasts were collected for light microscope observation and electromicroscope observation. Their expressions of proliferating cell nuclear antigen (PCNA) and the protein product of H-ras were examined by immunohistochemical staining. Results ①Mammary carcinoma incidence of group A(76.2%) was significantly higher than that of group B(40.9%), group C(45.5%) and group D(0%),P＜0.05, and there was no significant difference between group B and group C (P＞0.05), which indicated that quercetin could inhibit the occurrence of mammary carcinoma. ②Mean mammary tumor diameter of group A (2.37cm) was significantly larger than that of group B(1.82cm) and group C(1.71cm), P＜0.05, and there was no significant difference between group B and group C (P＞0.05), which indicated that quercetin could inhibit the growth of experimental mammary carcinoma. ③Immunohistochemical staining of PCNA showed significant difference between group A and group B, group A and group C (P＜0.05), with no significant difference between group B and group C (P＞0.05), which indicated that quercetin could inhibit the proliferation rate of tumor cells. ④Significant difference between group A and group B, group A and group C (P＜0.05), and no significant difference between group B and group C (P＞0.05), were noticed with immunohistochemical staining of H-ras protein product, which indicated that quercetin could inhibit the activity of Hras protein. Conclusion Quercetin could reduce the mammary carcinoma incidence and its degree of growth, and it may be related with its inhibitory effect on the activity of Hras and the proliferation of tumor cell.
Citation：
LIN Hui,WU Kainan,KONG Lingquan.. STUDY ON THE INHIBITORY EFFECTS OF QUERCETIN ON THE OCCURRENCE AND PROLIFERATION OF EXPERIMENTAL MAMMARY CARCINOMA. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2001, 8(5): 288-291. doi:
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Steven R, Donna S, Dieffenbach A, et al. Prediction of early relapse and shortened survived in patients with breast cancer by proliferating cell nuclear antigen score 〔Ｊ〕. Cancer, 1993; 71(19)∶3552.
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甘长清，吴凯南. 乳康片抑制实验性乳腺增生症及其机理的研究 〔Ｊ〕. 中国肿瘤临床，1999; 26(增刊)∶111.
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Ajik KV, Jeffrey AJ, Micheel NG, et al. Inhibition of 7,12dimethylbenz(a) anthraceneand Nnitrosomethylureainduced rat mammary cancer by dietay flavonol quercetin 〔Ｊ〕. Cancer Res,1998; 48(25)∶5754.
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张继增，刘华福，刘斌等. 乳腺增生病组织学分类及其与乳腺癌分类的研究 〔Ｊ〕. 中华病理学杂志，1995; 24(1)∶43.
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Larcher F, Robles AI, Duran H. Up regulation of vascular endothelial growth factor/vascular permeability factor in mouse skin carcinogenesis correlates with malignant progression and activated Hras expression levels 〔Ｊ〕. Cancer Res, 1996; 56(23)∶5391.
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张帆，张乃鑫. 大鼠化学诱发性乳癌的形态发生及其生物标记的研究状况 〔Ｊ〕. 中国肿瘤临床，1999; 26(1)∶64.
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Avila MA, Cansado J, Harter KV, et al. Quercetin as a modulator of the cellular neoplastic phenotype: effect on the expression of mutated Hras and p53 in rodent and human cells 〔Ｊ〕. Adv Exp Med Biol, 1996; 401(1)∶ 101.
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Csokay B, Prajda N, Weber G, et al. Molecular mechanism in the antiproliferative action of quercetin 〔Ｊ〕. Life Sci, 1997; 60(24)∶ 2157.
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- 1. 孔令泉，吴凯南. 槲皮素抗肿瘤作用研究进展 〔Ｊ〕. 四川医学，1999； 20(1)∶52.
- 2. Lu HA, Niggenmann B, Zanker KS, et al. Suppression of the proliferation and migration of oncogenic rasdependent cell linescultured in threedimensional collogen matrix by flavonoidstructure molecules 〔Ｊ〕. J Cell Res Clin Oncol,1996; 122(6)∶335.
- 3. Steven R, Donna S, Dieffenbach A, et al. Prediction of early relapse and shortened survived in patients with breast cancer by proliferating cell nuclear antigen score 〔Ｊ〕. Cancer, 1993; 71(19)∶3552.
- 4. 甘长清，吴凯南. 乳康片抑制实验性乳腺增生症及其机理的研究 〔Ｊ〕. 中国肿瘤临床，1999; 26(增刊)∶111.
- 5. 高进主编. 肿瘤学基础与实验 〔M〕. 第1版. 北京: 北京医科大学、中国协和医科大学联合出版社, 1992∶25~26.
- 6. 吴小华，蔡树模，张志毅等. 卵巢肿瘤中癌基因nm23H1，Cerb B2, ras和p53的蛋白表达与腹膜后淋巴结转移关系的研究 〔Ｊ〕. 肿瘤，1997; 17(3)∶134.
- 7. Ajik KV, Jeffrey AJ, Micheel NG, et al. Inhibition of 7,12dimethylbenz(a) anthraceneand Nnitrosomethylureainduced rat mammary cancer by dietay flavonol quercetin 〔Ｊ〕. Cancer Res,1998; 48(25)∶5754.
- 8. 张继增，刘华福，刘斌等. 乳腺增生病组织学分类及其与乳腺癌分类的研究 〔Ｊ〕. 中华病理学杂志，1995; 24(1)∶43.
- 9. 李树玲，刘奇，于金明等主编. 乳腺癌研究进展 〔C〕. 第二届国际乳腺癌学术会议论文汇编. 济南: 济南出版社，1996∶153～155.
- 10. Larcher F, Robles AI, Duran H. Up regulation of vascular endothelial growth factor/vascular permeability factor in mouse skin carcinogenesis correlates with malignant progression and activated Hras expression levels 〔Ｊ〕. Cancer Res, 1996; 56(23)∶5391.
- 11. 张帆，张乃鑫. 大鼠化学诱发性乳癌的形态发生及其生物标记的研究状况 〔Ｊ〕. 中国肿瘤临床，1999; 26(1)∶64.
- 12. Avila MA, Cansado J, Harter KV, et al. Quercetin as a modulator of the cellular neoplastic phenotype: effect on the expression of mutated Hras and p53 in rodent and human cells 〔Ｊ〕. Adv Exp Med Biol, 1996; 401(1)∶ 101.
- 13. Csokay B, Prajda N, Weber G, et al. Molecular mechanism in the antiproliferative action of quercetin 〔Ｊ〕. Life Sci, 1997; 60(24)∶ 2157.
