【Abstract】Objective To study the protective effects of anisodamine on liver ischemia-reperfusion injury in rats. Methods One hundred and sixty male Wistar rats were randomly divided into the normal control (n=10), ischemiareperfusion (n=50), normal saline (n=50) and anisodamine (n=50, 2.0 mg/kg) groups. The animals were killed 1, 3, 6, 12, 24 hours after ischemia induced for 60 minutes and followed by reperfusion. Plasma endothelin-1 (ET-1), hyaluronic acid (HA), glutamic-pyruvic transaminase enzyme (ALT) were measured, and the hepatic histopathologic alterations were also observed. Results The plasma ET-1, HA and ALT concentrations were markedly increased after liver ischemiareperfusion.The hepatic congestion was significantly obvious. An intravenous injection of anisodamine before ischemiareperfusion could decrease the plasma HA and ALT concentrations and relieve the hepatic congestion. Conclusion Anisodamine can improve hepatic microcirculatory disturbances after reperfusion and have hepatoprotictive effects on rat liver ischemia-reperfusion injury.
Citation：
DONG Manku,CUI Yan,CHEN Changwei,et al.. EXPERIMENTAL STUDY ON PROTECTIVE EFFECTS OF ANISODAMINE ON ISCHEMIA-REPERFUSION INJURY IN RAT LIVER. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2001, 8(5): 295-297. doi:
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邢卉春, 赵龙凤, 王守义. 山莨菪碱对大鼠急性肝损伤的保护作用及其机制 〔Ｊ〕. 中国危重病急救医学, 1998; 10(11)∶658.
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Wang WC, Lee YN. Effects of anisodamine and atropine on the microvasculature of liver, skeletal muscle and footpad skin in anesthetized rats 〔Ｊ〕. Yao Hsueh Hsueh Pao, 1992; 27(5)∶385.
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- 1. Nauta RJ, Tsimoyiannis E, Urib M, et al. Oxygenderived free radical in hepatic ischemia reperfusion injury in rat 〔Ｊ〕. Surg Gynecol Obstet, 1990; 171(2)∶120.
- 2. Yanagisawa M, Kurihara H, Kimuras E, et al. A novel potent vasoconstrictor peptide produced by vascular endothelial cells 〔Ｊ〕. Nature, 1988; 332(6163)∶411.
- 3. Itasaka H, Kishikawa K, Suehiro T, et al. Serum hyaluronic acid for the assessment of graft viability in porcine liver transplantation 〔Ｊ〕. Surg Today, 1994; 24(8)∶719.
- 4. Zhang JX, Pegoli W, Clemens MG, et al. Endothelin1 induces direct constriction of hepatic sinusoids 〔Ｊ〕. Am J Physiol, 1994; 266(29)∶G624.
- 5. Mitsuoka H, Suzuki S, Sakaguchi T, et al. Contribution of endothelin1 to microcirculatory impairment in total hepatic ischemia and reperfusion injury 〔Ｊ〕. Transplantation, 1999; 67(4)∶514.
- 6. Wang Y, Lawson JA, Jaeschke H. Differential effect of 2aminoethylisothiourea, an inhibitor of the inducible nitric oxide synthase, on microvascular blood flow and organ injury in models of hepatic ischemiareperfusion and endotoxemia 〔Ｊ〕. Shock, 1998; 10(1)∶20.
- 7. Menger MD, Richter S, Yamauchi J, et al. Role of microcirculation in hepatic ischemia/reperfusion injury 〔Ｊ〕. Hepatogastroenterology, 1999; 46(12)∶1452.
- 8. Cursio R, Gugenheim J, PanaiaFerrari P, et al. Improvement of normothermic rat liver ischemia/reperfusion by muramyl dipeptide 〔Ｊ〕. J Surg Res, 1998; 80(2)∶339.
- 9. Soejima Y, Yanaga K, Nishizaki T, et al. Effect of specific neutrophil elastase inhibitor on ischemia/reperfusion injury in rat liver transplantation 〔Ｊ〕. J Surg Res, 1999; 86(1)∶150.
- 10. 邢卉春, 赵龙凤, 王守义. 山莨菪碱对大鼠急性肝损伤的保护作用及其机制 〔Ｊ〕. 中国危重病急救医学, 1998; 10(11)∶658.
- 11. Wang WC, Lee YN. Effects of anisodamine and atropine on the microvasculature of liver, skeletal muscle and footpad skin in anesthetized rats 〔Ｊ〕. Yao Hsueh Hsueh Pao, 1992; 27(5)∶385.
