To study the expression of CTLA4Ig gene in diabetic rat and the effect of CTLA4Ig on longterm survival of the pancreatic islet. The rat pancreatic islet cell and muscle cell transfected with the cDNA for CTLA4Ig packaged with lipofectin vector. We examined the expression level of CTLA4Ig gene, T lymphocyte reaction and observed the expression of CTLA4Ig cDNA in diabetic rat and the action of CTLA4Ig in longterm survival of pancreatic islet transplanted and the transplanted rats. Results: The T lymphocyte reaction from peripheral intravenous blood at seventh day after surgery, the difference between two group was significant (Ｐ＜0.05). Only 2 out of 10 recipients of the experiment group (A) at the seventh day after pancreatic islet allograft had any detectable levels of CTLA4Ig, their concentration was 14 ng/ml, and 31 ng/ml. The average time of maintaining blood glycose in normal levels of the group A after pancreatic islets graft, 14.8±12.3 days, was significantly longer than 3.6±5.1 days of the control group (B) (Ｐ＜0.05). The average survival time of the group A, 24.0±10.8 days (the longest time was 45 days), was significantly longer than 11.8±4.8 days (the longest time was 21 days) of the group B (Ｐ＜0.01). Conclusions: The muclse cells and pancreatic islets of the recipient rat was transfected with CTLA4IgcDNA packaged with lipofectin, and CTLA4IgcDNA was expressed in recipient tissue, its expressed product CTLA4Ig make pancreatic islets transplanted and recipient rat survive longer significantly.
Citation：
Jin Shilong,Gu Hongguang,Wang Yaxiu,et al.. THE EFFECT OF CTLA4IG GENE ON PANCREATIC ISLET ALLOGRAFT FOR DIABETIC RAT. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1999, 6(6): 328-330. doi:
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Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
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Thomas AJ, Aimin T, Lisa MS, et al. The vivo mechanism of action of CTLA4Ig. J Immunol, 1996; 156(6)∶2294.
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Deborah J. Longterm survival of xenogenic pancreatic islet grafts induced by CTLA4Ig. Science, 1992; 257(3)∶709.
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Anita LG, Gregory SK, Ray VR, et al. Expression of CTLA4Ig by biolistcally transfected mouse islets promotes islets allograft survival. Transplantation, 1997; 63(7)∶1017.
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Alfred AC, Ming Y, Melissa MM, et al. Immunomodulation of pancreatic islet allografts in mice with CTLA4Ig secreting muscle cells. Transplantation, 1995; 59(9)∶1313.
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- 1. Groth CG. Immunosappression in pancreatic islet cell transpantation. Transpant Proc, 1994; 26(2)∶565.
- 2. 沈守祥， 董见虎， 毕艳军等. SD大鼠胰岛细胞培养技术的研究. 中华器官移植杂志， 1995； 16(4)∶158.
- 3. Anita LG, Greg SK, Ray VR, et al. Successful biolistic transformation of mouse pancreatic islets while preserving cellular function. Transplantation, 1996; 61(11)∶1567.
- 4. Matteo GL, Philip AP, Gregory LS, et al. Immunosuppressive effects of human CTAL4Ig in a nonhuman primate model of allogeneic pancreatic islet transplantation. J Immunol, 1997; 159(10)∶5187.
- 5. 杨廷彬，尹学念主编. 实用免疫学. 长春:长春出版社， 1994∶572～579.
- 6. Lin H, Hua L, Steven FB, et al. Longterm acceplance of major histocompalibility complex mismatched cardiac allografts induced by CTLA4Ig plus donor specific tranfusion. J Exp Med, 1993; 178(5)∶1801.
- 7. Thomas AJ, Aimin T, Lisa MS, et al. The vivo mechanism of action of CTLA4Ig. J Immunol, 1996; 156(6)∶2294.
- 8. Deborah J. Longterm survival of xenogenic pancreatic islet grafts induced by CTLA4Ig. Science, 1992; 257(3)∶709.
- 9. Anita LG, Gregory SK, Ray VR, et al. Expression of CTLA4Ig by biolistcally transfected mouse islets promotes islets allograft survival. Transplantation, 1997; 63(7)∶1017.
- 10. Alfred AC, Ming Y, Melissa MM, et al. Immunomodulation of pancreatic islet allografts in mice with CTLA4Ig secreting muscle cells. Transplantation, 1995; 59(9)∶1313.
