QUANTITATIVE STUDY OF RAS P21 EXPRESSION AND DNA CONTENT IN COLORECTAL CANCERS_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

Xu Liang ¹ , Lu Guangyu. ²

Department of Gastroenterology Surgery, Affiliated Hospital, Luzhou Medical College,Luzhou 646000;

Corresponding author：

Keywords：

Colorectal carcinoma; Ras p21; Deoxyribonucleic acid content

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The oncogene ras p21 expression and DNA content in 46 cases of colorectal tumor were analysed quantitatively with flow cytometry and cyto-immunofluorescence staining technique. The results showed that the positive rate of ras p21 expression was 65.7% and the rate of DNA aneuploid was 74.3% in colorectal carcinomas. Ras p21 expression was higher in colorectal adenocarcinomas than that of the adenomas and normal mucosa. DNA ploid and proliferative index had some association with ras p21 expresssion. Detection of ras p21 expression and DNA content in tumors may be helpful in predicting the outcome of colorectal cancer patients.

Citation： Xu Liang,Lu Guangyu.. QUANTITATIVE STUDY OF RAS P21 EXPRESSION AND DNA CONTENT IN COLORECTAL CANCERS. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1998, 5(1): 4-6. doi: Copy

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1. Hedley DM, Yonemura Y, Suzuki H, et al. Method for analysis of cellular DNA content of paraffin embedded pathological material using flow cytometry. J Histochem Cytochem, 1983; 31∶33.
2. Morkve O. Flow cytometric measurement of p53 protein expression and DNA content in paraffin embedded tissue from brochial carcinomas. Cytometry, 1991; 12∶438.
3. Barbacid M, Saito H, Clement JM, et al. Ras genes. Ann Rev Biochem,1987; 56∶779.
4. Bos JL. Ras oncogene in human cancer. Cancer Res, 1989; 49∶4682.
5. Scott N，Quirke P. Molecular biology of colorectal neoplasia. Gut, 1993; 34(3)∶289.
6. Miyahara M, Saito T, Kaketani K, et al. Clinical significance of ras p21 overexpression for patients with an advanced colorectal cancer. Dis Colon Rectum, 1991; 34∶1097.
7. Jansson DS. An immunohistochemical analysis of ras oncogene expression in epithelial neoplasms of the colon. Cancer, 1990; 65∶1329.
8. Wang Q. Biopathologic characteristics of DNA content in crypt cells of transitional mucosa adjacent to carcinoma of the rectum and rectosigmoid. Dis Colon Rectum, 1992; 25∶670.
9. Merkel DE， Mcguire WL. Ploidy proliferative activity and prognosis， DNA flow cytometry of solid tumors. Cancer, 1990; 65∶1194.
10. Barbara F, Chacho MS, Rosemen DL, et al. Multiparameter flow cytometric analysis of colon polyps. Am J Clin Pathol,1987; 87∶313.
11. Quirke P， Fozard JBJ, Dixon MF, et al. DNA aneuploidy in human colon carcinomas. Br J Cancer, 1986; 53∶477.
12. Sun XF. Ras p21 expression in relation to DNA ploidy, Sphase frac-tion and prognosis in colorectal adenocarcinomas.Cytometry, 1991; Suppl 5∶59.
13. Michelasis F, Erroi F, Roncella M, et al. Ras oncogene and the acquisition of metastasizing properties by rectal adenocarcinoma. Dis Colon Rectum, 1989; 32∶665.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

QUANTITATIVE STUDY OF RAS P21 EXPRESSION AND DNA CONTENT IN COLORECTAL CANCERS

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