Establishment and evaluation of a chronic pulmonary infection model due to Pseudomonas aeruginosa_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

YANG Qingmei , ZHANG Ping ,  SHEN Ce

Department of respiratory,Shanghai 6th people,s hospital,Shanghai 200233,China Corresponding author：Corresponding author：SHEN Ce，E-mail：lyshence@gmail.com;

Corresponding author：

SHEN Ce, Email: lyshence@gmail.com

Keywords：

Pseudomonas aeruginosa; Chronic pulmonary infection model; Silicone tube

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Objective To establish a rat model of chronic pulmonary infection by inoculating Pseudomonas aeruginosa to Sprague-Dawley(SD) rats.Metods Sixty SD rats were divided into 2 groups,ie.the P.aeruginosa group and the control group. Silicone tube precoated with P.aeruginosa was placed into the main bronchus. For the control group, sterile silicon tube was intubated. Results P ． aeruginosa was detected from lung tissue of rats in infected groups.Bacterial number was higher than 103cfu ／ g 28 days after inoculation.The pathological study showed fibrinous proliferation and granulomas formation in the lungs of infected rats 28 days after inoculation.Microscopy examination showed a inflammation predominantly with lymphocyte infiltration.In control group, no bacterial and pathological changes could be detected. Conclusions The animal model with P.aeruginosa chronic pulmonary infection can be established successfully by silicone tubes precoated with P.aeruginosa intubated into the main bronchus.

Citation： YANG Qingmei,ZHANG Ping,SHEN Ce. Establishment and evaluation of a chronic pulmonary infection model due to Pseudomonas aeruginosa. Chinese Journal of Respiratory and Critical Care Medicine, 2008, 08(1): 40-42. doi: Copy

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9.	Yu H，Hanes M，Chrisp CE，et al.Microbial pathogenesis in cystic fibrosis：pulmonary clearance of mucoid Pseudomonas aeruginosa and inflammation in a mouse model of repeated repiratory challenge.Infect Immun，1998，66：280-288.
10.	Imamura Y，Yanagihara K，Tomono K，et al.Role of Pseudomonas aeruginosa quorum-sensing systems in a mouse model of chronic respiratory infection.J Med Microbiol，2005，54：515-518.
11.	李倬哲，瞿介明，何礼贤，等.粒细胞减少大鼠铜绿假单胞菌肺部感染模型的建立及炎症反应研究.中华结核与呼吸杂志，2001，24：674-678.
12.	王文志，刘春涛，王曾礼，等.铜绿假单胞菌感染模型中CC16mRNA表达水平的研究.中国呼吸与危重监护杂志，2002，1：80-83.
13.	王炜芳，方向群，刘又宁.铜绿假单胞菌大鼠肺部感染模型的建立.中华医院感染学杂志，2007，17：499-501.
14.	Pearson JP，Feldman M.Pseudomonas aeruginosa cell-to-cell signaling is required for virulence in a model of acute pulmonary infection.Infect Immun，2000，68：4331-4334．.

1. Knapp B，Hundt E，Lens U，et a1.A recombinant hybrid outer membrarne protein for vaccination against Pseudmnmms aeruginosa.Vacine，1999，17：1663-1666．.
2. Katsunori Y，Kazunori T，Yukihiro K，et al.Role of elastase in a mouse model of chronic respiratory Pseudomonas aeruginosa infection that mimics diffuse panbronchiolitis.J Medl Microbiol，2003，52：531-535.
3. 柴栋，王睿，裴斐，等.铜绿假单孢菌生物被膜相关大鼠肺慢性感染免疫逃逸的研究.中国抗生素杂志2003，28：562-565.
4. 刘晓岚，宋志军，王立赞，等.铜绿假单胞菌QS系统缺陷对生物膜形成及大鼠肺部慢性感染的影响.济宁医学院学报，2007，30：100-103.
5. Yanagihara K，Tomono K，Sawal T，et al.Effect of clarithromycin on lymphocytes in chronic respiratory Pseudomonas aeruginosa infection.Am J Respir Crit Care，1997，155：337-342.
6. Jun-ichi K，Takayoshi T，Shigeru K.Combination therapy for chronic Pseudomonas aeruginosa respiratory infection associated with biofilm formation.J Antim Chem，2000，46：69-72.
7. Gilleland HE，Gilleland JR，Matthews-Greer JM.Outer Membrane Protein F Preparation of Pseudomonas aeruginosa as a Vaccine against Chronic Pulmonary Infection with Heterologous Immunotype Strains in a Rat Model.Infect Immun，1988，56：1017-1022.
8. 陆森全，王复融，尹秀.铜绿假单胞菌胞外粘液多糖结合疫苗的实验研究.中国人兽共患病杂志，2001，17：19-21.
9. Yu H，Hanes M，Chrisp CE，et al.Microbial pathogenesis in cystic fibrosis：pulmonary clearance of mucoid Pseudomonas aeruginosa and inflammation in a mouse model of repeated repiratory challenge.Infect Immun，1998，66：280-288.
10. Imamura Y，Yanagihara K，Tomono K，et al.Role of Pseudomonas aeruginosa quorum-sensing systems in a mouse model of chronic respiratory infection.J Med Microbiol，2005，54：515-518.
11. 李倬哲，瞿介明，何礼贤，等.粒细胞减少大鼠铜绿假单胞菌肺部感染模型的建立及炎症反应研究.中华结核与呼吸杂志，2001，24：674-678.
12. 王文志，刘春涛，王曾礼，等.铜绿假单胞菌感染模型中CC16mRNA表达水平的研究.中国呼吸与危重监护杂志，2002，1：80-83.
13. 王炜芳，方向群，刘又宁.铜绿假单胞菌大鼠肺部感染模型的建立.中华医院感染学杂志，2007，17：499-501.
14. Pearson JP，Feldman M.Pseudomonas aeruginosa cell-to-cell signaling is required for virulence in a model of acute pulmonary infection.Infect Immun，2000，68：4331-4334．.

Chinese Journal of Respiratory and Critical Care Medicine

Establishment and evaluation of a chronic pulmonary infection model due to Pseudomonas aeruginosa

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