Objective To observe the expression of alpha;A-and alpha;B-in retina after blue-light exposure.Methods Forty female Wistar rats were divided randomly into 4 groups:control group,and blue-light exposure for 6,12,and 24 hours groups, with 10 rats in each group. The rats in the control group were not intervened.The other three groups of rats were exposed to blue fluorescent lights for 6,12,and 24 hours respcetively. Then the rats were kept in darkness for 12 hours. The globes were enucleated after anaesthesia.The immunohistochemistry and Western blot were performed to detect the expression of alpha;A and alpha;B-crystallin in retina.Results The absorbance value (A value) of retina alpha;A-crystallin was 1.40573 plusmn;0.70748 in the control group, and were 4.317 51 plusmn;0.412 97, 7.397 08 plusmn;1.947 90, 9.634 32 plusmn;2.377 61, respectively in the other 3 groups; the difference among the groups was significant (F=24.569，P＜0.001). The A value of retina alpha;B-crystallin is 0.129 36 plusmn;0.033 93 in the control group, and were 0.507 17 plusmn;0.117 55, 7.345 43 plusmn;2.292 97, 4.042 26 plusmn;3.890 23, respectively in the other 3 groups; the difference among the groups was significant（F=40.102，P＜0.001）. The results of Western blot showed that the expression of alpha;A and alpha;B crystallin in groups with bluelight exposure was obviously higher than that in the control group.Conclusions Blue light may up-regulate the expression of alpha;A-and alpha;B-crystallin in rats prime; retina.
Citation： 蒋小爽,刘谊,张军军,邹明. Expression of αA and αB crystallin protein in retina after blue light exposure. Chinese Journal of Ocular Fundus Diseases, 2010, 26(1): 66-69. doi: Copy