QIAN Yishan , ZHANG Yongjin , YAN Xiang , et al.
  • Eye, ear, Nose and Throat Hospital, Fudan University, Shanghai 200031,China;
Phototherapy; Retinal pigment epithelium of eye; Endothelium, vascular; Growth sabstances; Cell culture
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Objective To investigate the impact of photodynamic therapy (PDT) with verteporfin on the expression of pigment epithelial derivative factor (PEDF) mRNA and vascular endothelial growth factor (VEGF) mRNA in adult retinal pigment epithelial (RPE) cells in vitro. Methods The changes of cellular viability before and after PDT were assessed by methyl thiazolyl tetrazolum (MTT) colorimetric assay. Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) was conducted to detect the expression of PEDF and VEGF mRNA in RPE cells before and after PDT. Results PDT caused the death of RPE cells. The cellular mortality was positively correlated with the power of photocoagulation and the concentration of verteporfin. Conclusion PDT could downregulate the expression of PEDF and VEGF mRNA in adult RPE cells in vitro, which may relate to the cure or relapse of subfoveal choroidal neovascular membrane after PDT. (Chin J Ocul Fundus Dis, 2006, 22: 256-260)

Citation： QIAN Yishan,ZHANG Yongjin,YAN Xiang,et al.. Influence of photodynamic therapy on expression of pigment epithelial derivative factor and vascular endothelial growth factor in adult human retinal pigment epithelial cells. Chinese Journal of Ocular Fundus Diseases, 2006, 22(4): 256-260. doi: Copy