Objective To explore the expressions of caudal-related homeodomain transcription factor-2 （CDX-2）
and tumor suppressor gene KAI-1 in colon carcinoma tissues and to analyze their clinical significances.
Methods Immu-nohistochemical SP method was used to detect the expressions of CDX-2 and KAI-1 in 50 cases of colon carcinoma tissues and corresponding adjacent tissues （from cancer tissue ≤2cm） and 25 cases of normal colon mucosa tissues. The relation-ships of the expressions of CDX-2 and KAI-1 to the clinicopathologic features were analyzed.
Results ①The positive rates of CDX-2 expression and KAI-1 expression in the colon carcinoma tissues were significantly lower than those in the normal colon mucosa tissues 〔CDX-2：34% （17/50） versus 88% （22/25）， P＜0.05；KAI-1：30% （15/50） versus 92% （23/25）， P＜0.05〕 and adjacent tissues of colon carcinoma 〔CDX-2：34% （17/50） versus 54% （27/50）， P＜0.05；KAI-1：30% （15/50） versus 58% （29/50）， P＜0.05〕， which in the adjacent tissues of colon carcinoma were significantly lower than those in the normal colon mucosa tissues （P＜0.05）. ②The positive expressions of CDX-2 and KAI-1 were related to lymph node metastasis， depth of invasion， and degree of tumor differentiation （P＜0.05）. ③Spearman rank correl-ation analysis showed that the CDX-2 expression was positively correlated with KAI-1 expression （rs=0.544， P＜0.01）.
Conclusions CDX-2 and KAI-1 may be closely related to the development， invasion， metastasis， and prognosis of colon carcinoma， the combination of CDX-2 and KAI-1 in evaluation of their function has a certain guiding significance in the treatment for colon carcinoma.
Citation：
LIU Jipan,YIN Changheng,SONG Mo,YAN Qinghui. Expressions of CDX-2 and Tumor Suppressor Gene KAI-1 in Colon Carcinoma Tissues and Their Clinical Significances. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2013, 20(11): 1263-1267. doi:
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Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
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-1399.
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- 1. Walsha T， Casadeia S， Ming K， et al. Mutations in 12 genes for inherited ovarian， fallopian tube， and peritoneal carcinoma identified by massively parallel sequencing[J]. PNAS， 2011，108，(44)：18032-18037.
- 2. Kang H， Chen IM， Wilson CS， et al. Gene expression classifiersfor relapse-free survival and minimal residual disease improve riskclassification and outcome prediction in pediatric B-precursor acutelymphoblastic leukemia[J]. Blood， 2010， 115(7)：1394-1405.
- 3. Saad RS， Ismiil N， Dubé V， et al. CDX-2 expression is a commonevent in primary intestinal-type rndocervical adenocarcinoma[J].Am J Clin Pathol， 2009， 132(4)：531-538.
- 4. Kenneth EH， Marco AM， Larissa GR， et al. Development of a mouse model for sporadic and metastatic colon tumors and its use in assessing drug treatment[J]. PNAS， 2010， 107(4)：1565-1570.
- 5. Malik FA， Sanders AJ， Kayan MA， et al. Effect of expressional alteration of Kai1 on breast cancer cell growth， adhesion， migration and invasion[J]. Cancer Genomics Proteomics， 2009， 6(4)：205-213.
- 6. Wei X， Li KC. Exploring the within- and between-class correlationdistributions for tumor classification[J]. Proc Natl Acad Sci， 2010， 7(15)：6737-6742.
- 7. 于秀文， 荣玮， 孙玉荣， 等. MUC2、CDX2在大肠肿瘤中的表达及临床意义[J]. 中国现代医学杂志， 2008， 18(21)：3115-3119.
- 8. 许良中， 杨文涛. 免疫组织化学反应结果的判断标准[J]. 中国癌症杂志， 1996， 6(4)：229-231.
- 9. Gross I， Duluc I， Benameur T， et al. The intestine-specific hom-eobox gene Cdx2 decreases mobility and antagonizes dissemination of colon cancer cells[J]. Oncogene， 2008， 27(1)：107-115.
- 10. Mallo GV， Rechreche H， Frigerio JM， et al. Molecular cloning， sequencing and expression of the mRNA encoding human Cdx1 and Cdx2 homeobox. Down-regulation of Cdx1 and Cdx2 mRNA expression during colorectal carcinogenesis[J]. Int J Cancer， 1997， 74(1)：35-44.
- 11. Sivagnanasundaram S， Islam I， Talbot I， et al. The homeobox gene CDX2 in colorectal carcinoma： a genetic analysis[J]. Br J Cancer， 2001， 84(2)：218-225.
- 12. Qualtrough D， Hinoi T， Fearon E， et al. Expression of CDX2 in normal and neoplastic human colon tissue and during differentiation of an in vitro model system[J]. Gut， 2002， 51(2)： 184-190.
- 13. Kaimaktchiev V， Terracciano L， Tornillo L， et al. The homeoboxintestinal differentiation factor CDX2 is selectively expressed ingastrointestinal adenocarcinomas[J]. Mod Pathol， 2004， 17(11)：.
- 14. Drummond F， Sowden J， Morrison K， et al. The caudal-typehomeobox protein Cdx-2 binds to the colon promoter of the carbonicanhydrase 1 gene[J]. Eur J Biochem， 1996， 236(2)： 670-681.
- 15. Sho M， Adachi M， Taki T， et al. Transmembrane 4 superfamily as a prognostic factor in pancreatic cancer[J]. Int J Cancer， 1998， 79(5)：509-516.
- 16. Yang X， Welch DR， Phillips KK， et al. KAI1， a putative markerfor metastatic potential in human breast cancer[J]. Cancer Lett， 1997， 119(2)：149-155.
- 17. Dong JT， Suzuki H， Pin SS， et al. Down-regulation of the KAI1 metastasis suppressor gene during the progression of human prostatic cancer infrequently involves gene mutation or allelic loss[J]. Cancer Res， 1996， 56(19)： 4387-4390.
- 18. White A， Lamb PW， Barrett JC. Frequent downregulation of the KAI1(CD82) metastasis suppressor protein in human cancer cell lines[J]. Oncogene， 1998， 16(24)：3143-3149.
- 19. Liu FS， Dong JT， Chen JT， et al. KAI1 metastasis suppressor protein is down-regulated during the progression of human endometrial cancer[J]. Clin Cancer Res， 2003， 9(4)：1393-1398.
- 20. -1399.
