• 1.Department of Pathology, Affiliated Hospital of Shandong Academy of Medical Sciences, Jinan 250031, Shandong Province, China;;
  • 2.Center Laboratory, Affiliated Hospital of Shandong Academy of Medical Sciences, Jinan 250031, Shandong Province,China;;
  • 3.Department of General Surgery, Affiliated Hospital of Shandong Academy of Medical Sciences, Jinan 250031,Shandong Province, China;;
YANG Xiangshan, Email: yangxiangshan163@163.com
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Objective  To explore the effect of membrane surface nucleolin (NCL) on activation of epidermal growth factor receptor (EGFR) signaling.
Methods  Immunohistochemistry was used to identify the expressions of membrane surface NCL or EGFR in pallilary thyroid carcinoma (PTC) tissues. The level of phosphorlated EGFR in TPC-1 cells was observed by Western blot. TPC-1 cells invasion capacity was detected by Transwell assay.
Results  The posi-tive expression rates of membrane surface NCL and EGFR in PTC tissues were 100% (56/56) and 80.4% (45/56) respe-ctively, while the expressions of NCL and EGFR were related with lymph node metastasis (P<0.05). There was posi-tive correlation between the expressions of NCL and EGFR (r=0.635, P<0.01). Western blot showed that anti-NCL or anti-EGFR of TPC-1 cells could inhibit the expression of phosphorlation EGFR (P<0.01). Transwell assay showed the number of membrane-invading cells were reduced significantly in anti-NCL group anti-EGFR group (P<0.01).
Conclusions  Membrane surface NCL may be a kind of indispensable component in activation of EGFR signaling, by which EGFR can participate in growth and invasion of tumors. NCL can be used as a target for developing a new field of tumor treatment.

Citation: YANG Xiangshan,FAN Kaixi,LI Chengjun,LI Aiping,ZHANG Jing,FENG Man.. Research on The Membrane Surface Nucleolin Participated The Activation of Epidermal Growth Factor Receptor Signaling. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2013, 20(10): 1117-1121. doi: Copy