Hemodynamic Observation on Acute Hindlimb Ischemia of Rats by Laser Doppler Flowmeter_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YANG Shengjia ¹ , CHEN Bing ¹ , TONG Zhu ¹ , ZHANG Shuwen ² , SHI Xiaolin ³ , XU Qing ³ , ZHANG Bin ¹ , ZHANG Jian ¹

1.Department of Vascular Surgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China;;
2.Regenerative Medicine Laboratory, Xuanwu Hospital, Capital Medical University, Beijing 100053, China;;
3.Department of Anatomy Histology and Embryology, Capital Medical University, Beijing 100034, China;

Corresponding author：

Keywords：

Laser Doppler flowmetry; Laser Doppler perfusion imaging; Hindlimb ischemia; Animal model; Rat

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

ObjectiveTo evaluate the dynamic changes of blood flow and blood pressure of acute hindlimb ischemia of rats by laser Doppler flowmetry (LDF) and laser Doppler perfusion imaging (LDPI). MethodsThe acute hindlimb ischemia model of rats was established by resection of rats femoral arteries of left hindlimb. The blood flow and blood pressure between operated and nonoperated hindlimbs were examined by LDF on 2, 7, 14, 28, and 49 d after operation. And the blood flow was evaluated by LDPI on 7 d after operation. ResultsAll rats survived after operation and no hindlimb necrosis occurred. The mean score was 2 on 14 d after operation and 1 on 49 d after operation. The ratio of blood flow between operated and nonoperated hindlimbs on 2 d after operation significantly increased from 1 to 1.31±0.439 (P=0.021). The ratio of blood flow on 7 d (0.82±0.538) and 14 d (0.93±0.294) after operation was significantly lower than that on 2 d after operation (P=0.032 and P=0.019), although the difference between the two former was not significant (P=0.502). Furthermore, the ratio of blood flow on 28 d after operation reached the bottom (0.41±1.970), which was obviously lower than that on 2, 7, and 14 d after operation (P=0.004, P=0.007, and P=0.006). The blood flow of operated hindlimbs recovered approximately the value before operation (0.98±0.093), which was significantly lower than that on 2 d (P=0.010), higher than that on 28 d (P=0.005), but not different from that on 7 d and 14 d after operation (P=0.126 and P=0.382). The ratio of blood pressure between operated and nonoperated hindlimbs on 2 d after operation significantly increased from 1 to 0.47±0.375 (P=0.031). The ratio of blood pressure decreased on 7 d after operation (0.44±0.118), which was not different from that on 2 d after operation (P=0.203). Furthermore, the ratio of blood pressure on 14 d after operation reached the bottom (0.35±0.115), which was obviously lower than that on 2 d and 7 d after operation (P=0.001 and P=0.036). On 28 d after operation, the ratio of blood pressure increased (0.54±0.146), which was significantly higher than that on 14 d after operation (P=0.008), while not different from that on 2 d (P=0.493) and 7 d after operation (P=0.551). The ratio of blood pressure recovered approximately the value before operation (0.97±0.094), which was significantly higher than that on 2, 7, 14, and 28 d (P=0.013, P=0.021, P=0.002, and P=0.031). ConclusionAcute hindlimb ischemia model of rats can be established by resection of rats femoral arteries of left hindlimb and the most serious stage of hindlimb ischemia is on 14-28 d after operation. LDF and LDPI are of importance for monitoring the dynamic changes of rats hindlimb ischemia after operation.

Citation： YANG Shengjia ,CHEN Bing,TONG Zhu,ZHANG Shuwen,SHI Xiaolin,XU Qing,ZHANG Bin,ZHANG Jian. Hemodynamic Observation on Acute Hindlimb Ischemia of Rats by Laser Doppler Flowmeter. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(2): 137-141. doi: Copy

1.	Lindsberg PJ, O’Neill JT, Paakkari IA, et al. Validation of laser Doppler flowmetry in measurement of spinal cord blood flow [J]. Am J Physiol, 1989, 257(2 Pt 2): H674H680.
2.	刘键, 李安之, 张光寅. 激光多普勒微循环血流计 [J]. 中国生物工程学报, 1983, 2(4): 250253.
3.	Schabauer AM, Booke TW. Cutaneous laser Doppler flowmetry: application and findings [J]. Mayo Clin Proc, 1994, 69(6): 564574.
4.	Zwagerman N, Sprague S, Davis MD, et al. Preischemic exercise preserves cerebral blood flow during reperfusion in stroke [J]. Neurol Res, 2010, 32(5): 523529.
5.	Li Z, Tam EW, Lau RY, et al. Post pressure response of skin blood flowmotions in anesthetized rats with spinal cord injury [J]. Microvasc Res, 2009, 78(1): 2024.
6.	Pettigrew LC, Kindy MS, Scheff S, et al. Focal cerebral ischemia in the TNFalphatransgenic rat [J]. J Neuroinflammation, 2008, 5: 47.
7.	Newman JM, Dwyer RM, StPierre P, et al. Decreased microvascular vasomotion and myogenic response in rat skeletal muscle in association with acute insulin resistance [J]. J Physiol, 2009, 587(Pt 11): 25792588.
8.	吴劲松, 陈衔城. 激光多普勒血流测定法在局部脑血流量监测中的应用 [J]. 国外医学脑血管疾病分册, 1999, 7(3): 162164.
9.	Rendell MS, Finnegan MF, Healy JC. The relationship of laserDoppler skin blood flow measurements to the cutaneous microvascular anatomy [J]. Microvas Res, 1998, 55(1): 313.
10.	杨剑, 刘博. 应用Perimed激光多普勒血流仪对家兔耳蜗血流的测量 [J]. 中国医学装备, 2007, 4(12): 4042.
11.	Line PD, Mowinicke P, Lien B, et al. Repeated measurement variation and precision of laser Doppler flowmetry measurements [J]. Microvas Res, 1992, 43(3): 285293.
12.	王宁, 陈兴新, 陈磊, 等. 血流多普勒信号采集与显示系统的设计 [J]. 中国医疗器械信息, 2008, 14(10): 4749.
13.	邵洪波, 姚月明, 张庆富, 等. 己酮可可碱干预随意皮瓣的成活激光多普勒血液灌流成像评价 [J]. 中国组织工程研究与临床康复, 2010, 14(5): 838842.
14.	Eichhorn W, Auer T, Voy ED. Diagnostic imaging of microcirculation of pedicled flapplasties of the mandibulofacial area using the laser Doppler scanner [J]. Handchir Mikrochir Plast Chir, 1995, 27(4): 176180.
15.	Murray AK, Herrick AL, King TA. Laser Doppler imaging: a developing technique for application in the rheumatic diseases [J]. Rheumatology (Oxford), 2004, 43(10): 12101218.
16.	Karamürsel S, Kayikioｇˇ lu A, Aksoy HM, et al. Dorsal visor flap in fingertip reconstruction [J]. Plast Reconstr Surg, 2001, 108(4): 10141018.
17.	黄晓元. 皮瓣移植在我国烧伤外科中的应用 [J]. 中华烧伤杂志, 2008, 24(5): 352354.
18.	霍鑫, 冯金炜, 刘方峰, 等. pEGFPC1/Akt体外转染骨髓间充质干细胞对后肢缺血大鼠血管生成的影响 [J]. 中国普外基础与临床杂志, 2008, 15(6): 402407.
19.	Pu LQ, Jackson S, Lachapelle KJ, et al. A persistent hindlimb ischemia model in the rabbit [J]. J Invest Surg, 1994, 7(1): 4960.
20.	Couffinhal T, Silver M, Zheng LP, et al. Mouse model of angiogenesis [J]. Am J Pathol, 1998, 152(6): 16671679.
21.	Ito WD, Arras M, Scholz D, et al. Angiogenesis but not collateral growth is associated with ischemia after femoral artery occlusion [J]. Am J Physiol, 1997, 273(3 Pt 2): H1255H1265.
22.	崔世军, 俞恒锡, 滕旭, 等. 家兔后肢缺血模型的制作 [J]. 中国比较医学杂志, 2003, 13(1): 3638.
23.	刘坤, 杨琨, 罗军, 等. 结扎腹主动脉加双侧腹壁阴部动脉在大鼠后肢缺血模型中的应用 [J]. 中国普外基础与临床杂志, 2010, 17(3): 258262.

1. Lindsberg PJ, O’Neill JT, Paakkari IA, et al. Validation of laser Doppler flowmetry in measurement of spinal cord blood flow [J]. Am J Physiol, 1989, 257(2 Pt 2): H674H680.
2. 刘键, 李安之, 张光寅. 激光多普勒微循环血流计 [J]. 中国生物工程学报, 1983, 2(4): 250253.
3. Schabauer AM, Booke TW. Cutaneous laser Doppler flowmetry: application and findings [J]. Mayo Clin Proc, 1994, 69(6): 564574.
4. Zwagerman N, Sprague S, Davis MD, et al. Preischemic exercise preserves cerebral blood flow during reperfusion in stroke [J]. Neurol Res, 2010, 32(5): 523529.
5. Li Z, Tam EW, Lau RY, et al. Post pressure response of skin blood flowmotions in anesthetized rats with spinal cord injury [J]. Microvasc Res, 2009, 78(1): 2024.
6. Pettigrew LC, Kindy MS, Scheff S, et al. Focal cerebral ischemia in the TNFalphatransgenic rat [J]. J Neuroinflammation, 2008, 5: 47.
7. Newman JM, Dwyer RM, StPierre P, et al. Decreased microvascular vasomotion and myogenic response in rat skeletal muscle in association with acute insulin resistance [J]. J Physiol, 2009, 587(Pt 11): 25792588.
8. 吴劲松, 陈衔城. 激光多普勒血流测定法在局部脑血流量监测中的应用 [J]. 国外医学脑血管疾病分册, 1999, 7(3): 162164.
9. Rendell MS, Finnegan MF, Healy JC. The relationship of laserDoppler skin blood flow measurements to the cutaneous microvascular anatomy [J]. Microvas Res, 1998, 55(1): 313.
10. 杨剑, 刘博. 应用Perimed激光多普勒血流仪对家兔耳蜗血流的测量 [J]. 中国医学装备, 2007, 4(12): 4042.
11. Line PD, Mowinicke P, Lien B, et al. Repeated measurement variation and precision of laser Doppler flowmetry measurements [J]. Microvas Res, 1992, 43(3): 285293.
12. 王宁, 陈兴新, 陈磊, 等. 血流多普勒信号采集与显示系统的设计 [J]. 中国医疗器械信息, 2008, 14(10): 4749.
13. 邵洪波, 姚月明, 张庆富, 等. 己酮可可碱干预随意皮瓣的成活激光多普勒血液灌流成像评价 [J]. 中国组织工程研究与临床康复, 2010, 14(5): 838842.
14. Eichhorn W, Auer T, Voy ED. Diagnostic imaging of microcirculation of pedicled flapplasties of the mandibulofacial area using the laser Doppler scanner [J]. Handchir Mikrochir Plast Chir, 1995, 27(4): 176180.
15. Murray AK, Herrick AL, King TA. Laser Doppler imaging: a developing technique for application in the rheumatic diseases [J]. Rheumatology (Oxford), 2004, 43(10): 12101218.
16. Karamürsel S, Kayikioｇˇ lu A, Aksoy HM, et al. Dorsal visor flap in fingertip reconstruction [J]. Plast Reconstr Surg, 2001, 108(4): 10141018.
17. 黄晓元. 皮瓣移植在我国烧伤外科中的应用 [J]. 中华烧伤杂志, 2008, 24(5): 352354.
18. 霍鑫, 冯金炜, 刘方峰, 等. pEGFPC1/Akt体外转染骨髓间充质干细胞对后肢缺血大鼠血管生成的影响 [J]. 中国普外基础与临床杂志, 2008, 15(6): 402407.
19. Pu LQ, Jackson S, Lachapelle KJ, et al. A persistent hindlimb ischemia model in the rabbit [J]. J Invest Surg, 1994, 7(1): 4960.
20. Couffinhal T, Silver M, Zheng LP, et al. Mouse model of angiogenesis [J]. Am J Pathol, 1998, 152(6): 16671679.
21. Ito WD, Arras M, Scholz D, et al. Angiogenesis but not collateral growth is associated with ischemia after femoral artery occlusion [J]. Am J Physiol, 1997, 273(3 Pt 2): H1255H1265.
22. 崔世军, 俞恒锡, 滕旭, 等. 家兔后肢缺血模型的制作 [J]. 中国比较医学杂志, 2003, 13(1): 3638.
23. 刘坤, 杨琨, 罗军, 等. 结扎腹主动脉加双侧腹壁阴部动脉在大鼠后肢缺血模型中的应用 [J]. 中国普外基础与临床杂志, 2010, 17(3): 258262.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Hemodynamic Observation on Acute Hindlimb Ischemia of Rats by Laser Doppler Flowmeter

Abstract Full text Figures/Tables Video References Cited by

Format

Content