Repression Effects of Anchor Chemric T Lymphocytes on Proliferation of Tumor Associated Glycoprotein 72 Positive Hepatocarcinoma Cells_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

XU Hongyong ¹ , XU Li ² , LI Kaizong ³

1.Department of Hepatobiliary General Surgery, The 451st Hospital of Chinese PLA, Xi’an 710054, Shaanxi Province, China;;
2.Institute of Digestive Disease, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China;;
3.Department of Hepatobiliary Surgery, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China;

Corresponding author：

Keywords：

Hepatocarcinoma; Tumor associated glycoprotein-72 antigen; Single chain antibody fragment variable; T lymphocyte

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ObjectiveTo investigate the method for generating anchor chemric T lymphocytes that can target tumor associated glycoprotein-72 (TAG72) antigen and analyze their repressive effects on proliferation of TAG72 positive hepatocarcinoma cells. MethodsFirstly, peripheral blood mononuclear cells (PBMCs) from healthy volunteers were isolated. And then, CD8+ T cells were isolated from PBMCs via magnetic activated cell sorting (MACS). These lymphocytes were transfected with recombinant vector, anti-TAG72-scFv-CD28-pcDNA3, through Lipofectamine2000 to gernerate anchor chimeric TAG72-specific CD8+ T cells. SMMC7721 (TAG72 positive) hepatocarcinoma cells were co-cultured with chimeric T lymphocytes and their cell cycles were analyzed by flow cytometry (FCM). ResultsAnchor chmeric T lymphcytes targetting TAG72 recognized TAG72 positive SMM7721 cells and repressive effects on their proliferation were observed by flow cytometry. ConclusionAnchor chmeric T lymphcytes targetting TAG72 on tumor surface can specifically recognize TAG72 positive hepatocarcinoma cells and may exert repressive effect on their proliferation.

Citation： XU Hongyong ,XU Li,LI Kaizong. Repression Effects of Anchor Chemric T Lymphocytes on Proliferation of Tumor Associated Glycoprotein 72 Positive Hepatocarcinoma Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(4): 370-373. doi: Copy

1.	徐宏勇, 徐立, 李开宗, 等. 抗TAG72抗原单链抗体的原核表达及对人肝癌的检测 [J]. 中国普外基础与临床杂志, 2010, 17(7): 699702.
2.	Ponnusamy MP, Venkatraman G, Singh AP, et al. Expression of TAG72 in ovarian cancer and its correlation with tumor stage and patient prognosis [J]. Cancer Lett，2007, 251(2): 247257.
3.	Chauhan SC, Vinayek N, Maher DM, et al. Combined staining of TAG72, MUC1, and CA125 improves labeling sensitivity in ovarian cancer: antigens for multitargeted antibodyguided therapy [J]. J Histochem Cytochem, 2007, 55(8): 867875.
4.	June CH, Bluestone JA, Nadler LM, et al. The B7 and CD28 receptor families [J]. Immunol Today, 1994, 15(7): 321331.
5.	Petersen CC, Petersen MS, Agger R, et al. Accumulation in tumor tissue of adoptively transferred T cells: A comparison between intravenous and intraperitoneal injection [J]. J Immunother, 2006, 29(3): 241249.
6.	徐宏勇, 徐立, 李开宗, 等. 靶向性肿瘤相关抗原TAG72的scFvCD28嵌合基因的真核表达载体的构建 [J].中国普外基础与临床杂志, 2002, 9(6): 398401.
7.	徐宏勇, 徐立, 高建宏, 等. 嵌合锚定T细胞致癌胚抗原阳性胃癌细胞的凋亡效应 [J]. 中华医学杂志, 2007, 87(15): 10531057.
8.	Willemsen RA, Weijtens ME, Ronteltap C, et al. Grafting primary human T lymphocytes with cancerspecific chimeric single chain and two chain TCR [J]. Gene Ther, 2000, 7(16): 13691377.
9.	Ychou M, Duffour J, Kramar A, et al. Clinical significance and prognostic value of CA724 compared with TAG72 and CA199 in patients with gastric cancer [J]. Dis Markers, 2000, 16(34): 105110.
10.	Hombach A, Heuser C, Marquardt T, et al. CD4+ T cells engrafted with a recombination immunorecrpter efficiently lyse target cells in a MHC antigen and Fas independent fashion [J]. J Immuno, 2001, 167(2): 10901095.
11.	Hombach A, Wieczarkowiecz A, Marquardt T, et al. Tumorspecific T cell activation by recombinant immunoreceptors: CD3 zeta signaling and CD28 costimulation are simultaneously required for efficient IL2 secretion and can be integrated into one combined CD28/CD3 zeta signaling receptor molecule [J]. J Immunol, 2001, 167(11): 61236130.
12.	Lee EY, Kim C, Kim MJ, et al. Signet ring cell carcinoma of the extrahepatic bile duct [J]. Gut Liver, 2010, 4(3): 402406.
13.	Gupta S, Sathishkumar S, Ahmed MM. influence of cell cycle checkpoints and p53 function on the toxicity of temozolomide in human pancreatic cancer cells [J]. Pancreatology, 2010, 10(5): 565579.
14.	Spitzner M, Emons G, Kramer F, et al. A gene expression signature for chemoradiosensitivity of colorectal cancer cells [J]. Int J Radiat Oncol Biol Phys, 2010, 78(4): 11841192.
15.	WilliamFaltaos S, Rouillard D, Lechat P, et al. Cell cycle arrest and apoptosis induced by oxaliplatin (LOHP) on four human cancer cell lines [J]. Anticancer Res, 2006, 26(3A): 20932099.
16.	WilliamFaltaos S, Rouillard D, Lechat P, et al. Cell cycle arrest by oxaliplatin on cancer cells [J]. Fundam Clin Pharmacol, 2007, 21(2): 165172.

1. 徐宏勇, 徐立, 李开宗, 等. 抗TAG72抗原单链抗体的原核表达及对人肝癌的检测 [J]. 中国普外基础与临床杂志, 2010, 17(7): 699702.
2. Ponnusamy MP, Venkatraman G, Singh AP, et al. Expression of TAG72 in ovarian cancer and its correlation with tumor stage and patient prognosis [J]. Cancer Lett，2007, 251(2): 247257.
3. Chauhan SC, Vinayek N, Maher DM, et al. Combined staining of TAG72, MUC1, and CA125 improves labeling sensitivity in ovarian cancer: antigens for multitargeted antibodyguided therapy [J]. J Histochem Cytochem, 2007, 55(8): 867875.
4. June CH, Bluestone JA, Nadler LM, et al. The B7 and CD28 receptor families [J]. Immunol Today, 1994, 15(7): 321331.
5. Petersen CC, Petersen MS, Agger R, et al. Accumulation in tumor tissue of adoptively transferred T cells: A comparison between intravenous and intraperitoneal injection [J]. J Immunother, 2006, 29(3): 241249.
6. 徐宏勇, 徐立, 李开宗, 等. 靶向性肿瘤相关抗原TAG72的scFvCD28嵌合基因的真核表达载体的构建 [J].中国普外基础与临床杂志, 2002, 9(6): 398401.
7. 徐宏勇, 徐立, 高建宏, 等. 嵌合锚定T细胞致癌胚抗原阳性胃癌细胞的凋亡效应 [J]. 中华医学杂志, 2007, 87(15): 10531057.
8. Willemsen RA, Weijtens ME, Ronteltap C, et al. Grafting primary human T lymphocytes with cancerspecific chimeric single chain and two chain TCR [J]. Gene Ther, 2000, 7(16): 13691377.
9. Ychou M, Duffour J, Kramar A, et al. Clinical significance and prognostic value of CA724 compared with TAG72 and CA199 in patients with gastric cancer [J]. Dis Markers, 2000, 16(34): 105110.
10. Hombach A, Heuser C, Marquardt T, et al. CD4+ T cells engrafted with a recombination immunorecrpter efficiently lyse target cells in a MHC antigen and Fas independent fashion [J]. J Immuno, 2001, 167(2): 10901095.
11. Hombach A, Wieczarkowiecz A, Marquardt T, et al. Tumorspecific T cell activation by recombinant immunoreceptors: CD3 zeta signaling and CD28 costimulation are simultaneously required for efficient IL2 secretion and can be integrated into one combined CD28/CD3 zeta signaling receptor molecule [J]. J Immunol, 2001, 167(11): 61236130.
12. Lee EY, Kim C, Kim MJ, et al. Signet ring cell carcinoma of the extrahepatic bile duct [J]. Gut Liver, 2010, 4(3): 402406.
13. Gupta S, Sathishkumar S, Ahmed MM. influence of cell cycle checkpoints and p53 function on the toxicity of temozolomide in human pancreatic cancer cells [J]. Pancreatology, 2010, 10(5): 565579.
14. Spitzner M, Emons G, Kramer F, et al. A gene expression signature for chemoradiosensitivity of colorectal cancer cells [J]. Int J Radiat Oncol Biol Phys, 2010, 78(4): 11841192.
15. WilliamFaltaos S, Rouillard D, Lechat P, et al. Cell cycle arrest and apoptosis induced by oxaliplatin (LOHP) on four human cancer cell lines [J]. Anticancer Res, 2006, 26(3A): 20932099.
16. WilliamFaltaos S, Rouillard D, Lechat P, et al. Cell cycle arrest by oxaliplatin on cancer cells [J]. Fundam Clin Pharmacol, 2007, 21(2): 165172.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Repression Effects of Anchor Chemric T Lymphocytes on Proliferation of Tumor Associated Glycoprotein 72 Positive Hepatocarcinoma Cells

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